Description
0112-0127 B
37
Optical System
The instrument uses excitation and emission filter wheels to decrease
interference by stray light, thus augmenting the wavelength selection
that is provided by the monochromators. Two independent, single-
channel reading heads can service top and bottom reading requirements.
Both the top reading head and bottom reading head support coaxial
excitation and emission beams.
The instrument’s electrical, firmware, and optical designs incorporate
many features that work together to virtually eliminate instrument-
based day-to-day and instrument-to-instrument variations in measured
fluorescence values.
For more detail of the optical design and an illustration of the optical
system, see
Bottom and Top Reading
Switching to bottom or top reading capability is activated through
software. No manual positional switching of the read-head is required.
Bottom reading allows for well scanning ability maximizing the sampling
area for 6-well, 12-well, 24-well, 48-well, 96-well, and 384-well
microplates. In addition, bottom reading enables concurrent reagent
addition to monitor fast kinetic reactions such as calcium mobilization.
Availability of top or bottom reading functions varies depending on the
detection mode.
Note:
Clear-bottom plates must be used for bottom reading. Bottom
reading is intended for cell-based assays.
FlexStation_manual.book Page 37 Tuesday, June 25, 2013 10:22 AM
