FlexStation 3 Benchtop Multi-Mode Microplate Reader User Guide
158
0112-0127 B
Table B-6:
Time-Resolved Fluorescence Performance
Item
Specification
Sensitivity
100 fM europium in 96-well or 384-well microplates
(top read)
Wavelength range
250 nm to 850 nm
Bandwidth (excitation,
emission)
9 nm, 15 nm
Precision data collection 1 to 100 flashes; delay of 0
μ
s to 600
μ
s before read;
integration time selectable 50
μ
s to 1500
μ
s.
Table B-7:
Luminescence Photometric Performance
Item
Specification
Sensitivity
Sensitivity < 2 fg/well for firefly luciferase in 96-well
and 384-well microplates (top read)
Wavelength range
250 nm to 850 nm
Crosstalk
< 0.3% in 96-well and 384-well microplates.
Table B-8:
Photometric Analysis Mode Using SoftMax Pro Software
Item
Specification
Data expression
Absorbance: OD or %Transmittance
Fluorescence: Relative Fluorescence Units (RFU)
Luminescence: Relative Luminescence Units (RLU)
Wavelengths per read
1 to 4 wavelengths per microplate read
Read types
Endpoint
Kinetic and kinetic graphics
Spectral scan: 200 nm to 1000 nm
Well scan (absorbance or fluorescence)
Table B-9:
Measurement Time (Calibration Off)
Item
Specification
Absorbance,
single wavelength
96 wells: 18 seconds
384 wells: 49 seconds
Fluorescence,
single wavelength
96 wells: 17 seconds
384 wells: 48 seconds
FlexStation_manual.book Page 158 Tuesday, June 25, 2013 10:22 AM
