Operating Procedures
0112-0127 B
85
AutoCalibrate
This AutoCalibrate check box allows you to disable or enable automatic
calibration. The default is enabled. Turn automatic calibration off to
allow the instrument to begin or complete readings more quickly.
The instrument maintains the most recent automatic calibration settings
in memory (NVRAM) until another automatic calibration is performed.
Figure 3-21:
AutoCalibrate Selection
Settling Time
Settling Time is the delay time between reader motion and initiation of
reading. In Fluorescence Polarization, the settling time happens before
each well is read. However, in all other read modes, the settling time
happens only after the microplate has been moved to begin the read of
another column. This delay allows time for the meniscus motion to
cease, potentially improving precision, especially in the low-density,
high-volume microplates with fewer than 96 wells.
AutoRead
AutoRead, if checked, causes the reader to read the same microplate
over and over, without user intervention. Several different Plate sections,
with the same or different setups, can be defined in one experiment
protocol. Starting the read in the first protocol results in the microplate
being read until it reaches the last Plate section, after which it
automatically stops.
Note:
Allow the instrument to perform an automatic calibration of at
least one microplate before you disable this function.
CAUTION!
To prevent data loss, make sure that you turn off the
AutoRead function for the last Plate section.
FlexStation_manual.book Page 85 Tuesday, June 25, 2013 10:22 AM
