FlexStation 3 Benchtop Multi-Mode Microplate Reader User Guide
14
0112-0127 B
The system has five assay types available which include Flex, Endpoint,
Kinetic, Spectrum, and multi-point Well Scan. Flex mode assay enables
users to transfer fluid from the source read plate while immediately
reading a fast kinetic assay that uses absorbance, fluorescence, or
luminescence detection technologies. Alternatively, the pipettor head
can be used to transfer fluid before an endpoint or kinetic assay to
automate fluid transfer and minimize user interaction.
In addition to fluid transfer, well contents can be mixed automatically by
shaking before each read cycle, making it possible to perform kinetic
analysis of solid-phase, enzyme-mediated reactions.
Top detection in microplates is available for all fluorescence and
luminescence readings.
Bottom detection is available for all assay types
except fluorescence polarization. When reading absorbance at
wavelengths below 340 nm, special UV-transparent, disposable or quartz
microplates that allow transmission of the far ultraviolet spectra must be
used.
A plate drawer adapter is provided with the reader. The adapter is
required for optimum performance when reading from the top or
bottom of standard 96-well and 384-well microplates in all read types,
including absorbance.
Variations in measured fluorescence values are virtually eliminated by
internal compensation for detector sensitivity, photomultiplier tube
voltage, and excitation source intensity.
Using the FlexStation 3 instrument with the PathCheck® Pathlength
Measurement Technology allows normalization of variable well volumes
to absorbance readings. In addition PathCheck technology allows for
pipettor validation, including the online 8-channel and 16-channel
pipettors, and to compare experiments from different days.
Temperature in the microplate chamber is isothermal, both at ambient
and when the incubator is turned on. When the incubator is on, the
reading chamber temperature can be controlled from 2°C above ambient
room temperature to 45°C. Please note that the temperature of the
fluidics module is not regulated and that it is recommend that any
microplates or tips should be at the desired temperature before placing
them inside the instrument.
FlexStation_manual.book Page 14 Tuesday, June 25, 2013 10:22 AM
