FlexStation 3 Benchtop Multi-Mode Microplate Reader User Guide
108
0112-0127 B
Results
The optimal excitation and emission wavelengths are those
determined in
Perform the Excitation Scan on page 105
in
Perform Emission Scan #2: on page 107
.
Comments
In Endpoint or Kinetic fluorescence reads, the Autofilter
feature generally selects the same cutoff filter wavelength as
the above optimization method. If desired, however, you can
specify the cutoff filters manually.
For emission wavelengths less than 325 nm, experimental
iteration is usually the best method of determining the
optimal emission and excitation wavelengths. Begin
optimization by performing the previous procedures. Try
emission and excitation wavelength combinations with the
325 nm cutoff or with no cutoff filter. Similarly, for excitation
wavelengths greater than 660 nm, try emission and
excitation wavelength combinations with the 695 nm cutoff
or with no cutoff filter.
Figure 3-37:
Effects of cutoff filters on fluorescein. Emission was
scanned from 490 nm to 560 nm; excitation was fixed at 485 nm.
FlexStation_manual.book Page 108 Tuesday, June 25, 2013 10:22 AM
