FlexStation 3 Benchtop Multi-Mode Microplate Reader User Guide
164
0112-0127 B
Excitation Filter
An excitation filter is a band pass filter that reduces the amount of
extraneous lamp excitation light before the excitation monochromator.
In endpoint reads and emission spectral scans, selection of excitation
filter is automatic. In excitation spectrum scans, the user has the choice
of “no excitation filter” (for smoother scans) or “auto excitation filter” in
which case there might be slight glitches in the spectrum at the
wavelengths where filter changes occur.
Excitation Spectrum Scan
An excitation spectrum scan measures fluorescence at a single emission
wavelength for the emitted light across a spectrum of excitation
wavelengths. The default value reported for each well is the wavelength
of maximum fluorescence excitation in RFU.
Fluorophore
A fluorophore is a material that absorbs light energy of a characteristic
wavelength, undergoes an electronic state change, and instantaneously
emits light of a longer wavelength.
Gain
Gain is the amount of increase in signal power expressed as the ratio of
output to input for a photomultiplier tube (PMT).
Incubator
In the SoftMax Pro Software, clicking Control > Incubator or clicking the
incubator button opens a dialog allowing you to start or stop temperature
regulation and to select an elevated temperature for the reading
chamber.
Instrument Setup
The instrument setup defines the parameters (mode, wavelengths,
automatic mixing, run time, read interval, and so on) used to read the
microplate.
FlexStation_manual.book Page 164 Tuesday, June 25, 2013 10:22 AM
