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1

Getting Familiar with Your TriPlus RSH

MHE Station

Thermo Scientific

TriPlus RSH User Guide

51

We will look at four examples

situations

of equilibrium between the condensed (solid or

liquid) phase and the gas phase (the headspace). The examples will be shown in increasing
complexity with respect to the equilibrium distribution and removal of the headspace between
subsequent extractions.

In each of the four cases described below, the complexity of the equilibrium distribution and
of the removal of the headspace between subsequent extractions will be increased. The final
conclusion is that the MHE method will work in most cases, with the only prerequisite that
the distribution of the analyte between the gas and the condensed phases is actually governed
by an equilibrium.

Situation 1

The equilibrium between the solid phase and the gas phase is assumed to be a
50/50-distribution. In the course of each extraction,

all

of the headspace is

removed/exchanged.

The measured area of the analyte peak is

A

.

A(ref )

— Area we would measure if all of the analyte were in the headspace (corresponding to

the measured signal from a similar gas standard).

A(0)

— Measured area of the analyte peak from the first extraction.

With 50% of the analyte present in the headspace, this amounts to:

A(0) = ½ * A(ref )

The total area that is determined over a number of subsequent extractions is:

A(tot) = A(0) + ½ * A(0) + ¼ * A(0) + 1/8 * A(0) + ……, or

A(tot) = A0 / (1-½) = 2 * A0

However, A(0) = ½ * A(ref ) and therefore:

A(tot) = A(ref )

Thus, it would be correct to determine the total amount of analyte by comparing the
measured sum of analyte areas to a calibration measurement with data from a single
determination of a gas standard.

Situation 2

The equilibrium between the condensed phase and the headspace is assumed to be 25/75
(with 75% in the gas phase). Furthermore, we assume that the entire headspace is exchanged
during the course of each extraction step.

The measured area of the analyte peak is

A

.

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Summary of Contents for TriPlus RSH

Page 1: ...Thermo Scientific TriPlus RSH Robotic Sample Handling User Guide P N 31709620 Ninth Edition December 2015 ...

Page 2: ...evious information received by the purchaser Thermo Fisher Scientific Inc makes no representations that this document is complete accurate or error free and assumes no responsibility and will not be liable for any errors omissions damage or loss that might result from any use of this document even if the information in the document is followed properly This document is not part of any sales contra...

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Page 5: ...mpliance with applicable domestic and international regulations Thermo Fisher Scientific declares under sole responsibility that the product as originally delivered complies with the requirements of the following applicable European Directives and carries the CE marking accordingly Low Voltage Directive 2006 95 EC EMC Directive 2004 108 EC Machinery Directive 2006 42 EC and conforms with the follo...

Page 6: ...pliance with international regulations Use of this instrument in a manner not specified by Thermo Fisher Scientific could impair any protection provided by the instrument Notice on the Susceptibility to Electromagnetic Transmissions Do not use radio frequency transmitters such as mobile phones in close proximity to the instrument THIS DEVICE COMPLIES WITH PART 15 OF THE FCC RULES OPERATION IS SUBJ...

Page 7: ...dieses Produkt durch diese Firmen wiederverwertet oder entsorgt werden kann Mehr Information über die Einhaltung dieser Anweisungen durch Thermo Fisher Scientific über die Verwerter und weitere Hinweise die nützlich sind um die Produkte zu identifizieren die unter diese RoHS Anweisung fallen finden sie unter www thermoscientific com rohsweee Conformité DEEE Ce produit doit être conforme à la direc...

Page 8: ...ea y estas empresas deberían reciclar o eliminar este producto Referirse a www thermoscientific com rohsweee para una mayor información sobre la conformidad de Thermo Fisher Scientific con estas Directivas y para las empresas de reciclaje en su país IMPORTANT The symbol indicates the product must not be disposed of with the normal household wastes Correct disposal of this product prevents any pote...

Page 9: ...eral Considerations xx Electrical Hazards xxi Laser Safety Information xxii Other Hazards xxiii Working with Toxic or other Harmful Compounds xxv Biological Hazards xxv Maintenance xxvi Disposal xxvi Chapter 1 Getting Familiar with Your TriPlus RSH 1 Instrument Basics 2 Definition of Terms Naming Conventions and Start Screen Icons 3 Definition of Terms 3 Naming Conventions 6 Start Screen Icons 7 T...

Page 10: ...ools 27 Syringes for SPME Tools 29 Definition of SPME Penetration Depth 29 ITEX 2 Tool 30 Control Interface 33 Definitions of Active and Passive Hardware Modules 35 Active Versus Passive Module 35 Active Versus Composite Module 36 Modules Description and Specifications 37 TR Station 38 ATC Station 39 Standard Wash Station 40 Large Volume Wash Station 41 Large Solvent Station 41 Fast Wash Station 4...

Page 11: ...lectronic Box for OC Injector Actuator 75 Handheld Controller 76 Menu Screen 76 Menu Screen Status Bar Symbols 77 Function Keys and Buttons 78 Scroll Wheel and Enter Button 79 Handheld Controller Versus the Virtual Handheld Controller 79 Chapter 2 TriPlus RSH Basic Informations 81 Starting the TriPlus RSH 82 How to Start the TriPlus RSH 82 Short cuts from Startup Screen 84 Access Levels 85 User Le...

Page 12: ...ls 135 Naming Convention and Relationship between Software Classes Tray Holder Trays Tray Types and Cap Type 141 Tray Holders 142 Tray Types 146 Trays 158 Vial Types 160 Vials 164 Wash Stations 168 Tray Slots 174 Unknown 174 Valves 174 Setup Menu Item Set Date and Time 176 Setup Menu Item Set TriPlus RSH Name 177 Setup Menu Item Setup Network 178 Chapter 4 Maintenance Menu Items 181 Maintenance Me...

Page 13: ...work 196 Calibrate Motors 199 Teach Tool Station 202 Configure SPME Tool 207 Set Reference Point 209 Calibrate Tools 210 Setup Module 213 Teach Module 214 Check Teaching Modules 218 Create a Backup File 220 Create New Tray System Item 222 Teach Home Position 224 Changing Home Position 224 Chapter 6 Analytical Troubleshooting 227 General Points Regarding Chromatographic Effects 228 Headspace Techni...

Page 14: ......

Page 15: ... RSH Basic Informations provides basic informations to start the TriPlus RSH and for using the Handheld Controller Chapter 3 Setup Menu Item describes the options of the Setup Menu operated either with the Virtual Handheld Controller or directly using the installed Handheld Controller Chapter 4 Maintenance Menu Items describes the options of the Maintenance Menu operated either with the Virtual Ha...

Page 16: ...Power Modules are required Detailed instrument specifications are in the Product Specification or Product Brochure Contacting Us Thermo Fisher Scientific provides comprehensive technical assistance worldwide and is dedicated to the quality of our customer relationships and services Use http www thermoscientific com address for products information address for products information Use http www gc g...

Page 17: ...ons that might cause personal injury instrument damage or both You can find the following safety symbols on your instrument or in this guide IMPORTANT Highlights information necessary to prevent damage to software loss of data or invalid test results or might contain information that is critical for optimal performance of the system Note Emphasizes important information about a task Tip Helpful in...

Page 18: ...ND CHEMICAL HAZARD Indicates that chemical damage or physical injury could or might occur HARMFUL Indicates that the presence of harmful material will could or might occur INSTRUMENT DAMAGE Indicates that damage to the instrument or component might occur This damage might not be covered under the standard warranty LIFTING HAZARD Indicates that a physical injury could or might occur if two or more ...

Page 19: ...if the instrument is improperly used or if unsafe actions occur This symbol and another appropriate safety symbol alerts you to an imminent or potential hazard that could cause personal injury Table 1 Instrument Marking and Symbols Sheet 1 of 2 Symbol Description Direct Current Alternating Current Both direct and alternating current Three phase alternating current Earth ground terminal Protective ...

Page 20: ...t not expressly approved by the party responsible for compliance could void your s authority to operate the equipment Be aware that if the equipment is used in a manner not specified by the manufacturer the protective and safety features of the equipment might be impaired The repair of instrument failures caused by operation in a manner not specified by the manufacturer is expressly excluded from ...

Page 21: ...e of the instrument Do not connect the TriPlus RSH to power lines that supply devices of a heavy duty nature such as motors refrigerators and other devices that can generate electrical disturbances Use only fuses of the type and current rating specified Do not use repaired fuses and do not short circuit the fuse holder The supplied power cord must be inserted into a power outlet with a protective ...

Page 22: ...s applied to the TriPlus RSH After the power is turned off wait approximately 30 seconds before you disconnect an assembly The instrument includes a number of integrated circuits These circuits might be damaged if exposed to excessive line voltage fluctuations power surges or electrostatic charges or both Never try to repair or replace any components of the instrument without the assistance of a T...

Page 23: ...peration Turn off the power to the TriPlus RSH if you must reach inside a mechanically powered system with moving parts To avoid injury observe safe laboratory practice when handling solvents changing tubing or operating the TriPlus RSH Know the physical and chemical properties of the solvents you use See the MSDS Material Safety Data Sheets from the manufacturer of the solvents being used When us...

Page 24: ... greater than 60 C is applied consider the vapor pressure of the solvent used to ensure that no excessive pressure builds up This is important when using a temperature above 100 C and especially at the maximum temperature of 200 C Be aware that solid materials can also contain volatile compounds such as water humidity which could cause build up of excess vapor pressure Do not reuse headspace vials...

Page 25: ... material The appropriate warning labels are included with the shipment of the instrument It is your responsibility to label the relevant parts of the instrument When working with biohazardous materials it is your responsibility to fulfill the following mandatory requirements Instructions on how to safely handle biohazardous material must be provided Operators must be trained and made aware of the...

Page 26: ...ons When preparing for decontamination ensure that the solvent or chemical to be used will not damage or react with the surface dye color of the instrument table or other nearby objects If in doubt please contact your Thermo Fisher Scientific representative to verify the compatibility of the type or composition of solvents with the TriPlus RSH Disposal Do not dispose of this equipment or parts the...

Page 27: ...ls Head TriPlus RSH Tools LS Tool HS Tool SPME 1 and SPME 2 Tools ITEX 2 Tool Control Interface Definitions of Active and Passive Hardware Modules Modules Description and Specifications TR Station ATC Station Standard Wash Station Large Volume Wash Station Large Solvent Station Fast Wash Station Agitator Vortexer MHE Station SPME Conditioning Station Barcode Reader Standard Tray Holder Liquid Cool...

Page 28: ...le performance providing easy access to essential information which might be requested by inspection authorities A unique ATC Station allows an automated change of Tools syringes with different volumes or different tool types for advanced sampling preparation liquid handling dilutions derivatization steps or any other time consuming repetitive step This added versatility alongside the increase in ...

Page 29: ...r via active control An active module requires a device firmware which is part of the RSH Firmware An active module is recognized when connected to the BUS it can be de activated but not deleted Passive Module A static module e g a non temperature programmable tray holder does not require any control or checks through the BUS A passive module requires an object template which is part of the RSH Fi...

Page 30: ...ack RSH Firmware Starting with software version 2 0 the Control Software is renamed to Firmware The definition is identical as described above with Control Software Device Firmware The software for an active module The device firmware is part of the RSH Firmware Activity The lowest level of command available with the RSH Firmware Activities depend on the Application Software version and are downlo...

Page 31: ...re defined by designating the tray type and the Tray Holder Tray names are used to identify the sample source within a Job Well Plate A generic term used for all types of well plates The most common well Plates are deepwell plates or microtiter plates The well plates have a standardized footprint The tray holder drawers are designed to accept well plates Passive tray holders for the TriPlus RSH ha...

Page 32: ... 15 positions 3 x 5 Pattern Regular for 10 and 20 mL vials with OD 23 mm and height 47 78 mm Tray 54 VT54 Tray with same footprint as Well Plate 54 positions 6 x 9 Pattern Regular for 2 mL vials with OD 12 mm and height 34 mm Tray 70 VT70 Tray with same footprint as Well Plate 70 positions 7 x 10 Pattern Regular for 1 mL vials with OD 7 5 mm and height 45 mm Viewing window in position 68 for needl...

Page 33: ...iameter 7 0 mm MTP384 Well Plate Type Microtiter Plate MT384 384 positions 24 x 16 Pattern Regular Plate Height 14 4 mm Well Cavity Depth 11 5 mm Well Cavity Diameter 3 7 mm Table 1 Example of Tray Types Naming Convention Sheet 2 of 2 Tray Type Tray Description Table 2 Start Screen Icons Sheet 1 of 3 Icon Description Tool and Tool Change Station LS Tool Syringe tool for liquid Injections HS Tool H...

Page 34: ...inge Module Passive Module Tray Holder rectangular Liquid Cooled Tray Holder Standard Wash Station 5x10 mL Vials Large Solvent Wash Station 2 x 100 mL bottles and 1 Waste Position Solvent Station Solvent reservoir station for 3 x 100 mL bottles Tray rectangular Tray to hold vials with volume from 1 to 40 mL Vials set of vials Vial single vial Vial Type CV Solvent Reservoir Bottle Vial Type SR Inje...

Page 35: ...de 9 Synchronization and Signals Input and Output Signals Input Signal Output Signal Chromatographic System Specifies synchronization of Input and Output Signals Cryo Trap and Delay Time for a Fake Injection for an analytical system General Icons TriPlus RSH System Head Injection unit Table 2 Start Screen Icons Sheet 3 of 3 Icon Description ...

Page 36: ...omponents are chosen by the operator according to their own analytical demands Choosing the appropriate components TriPlus RSH sampler may be configured for Liquids LS Headspace HS or Solid Phase Micro Extraction SPME or In tube Extraction ITEX Sampling Unit The sampling unit consists of the following major components Sampler Support Constitutes the supporting base allowing the installation of the...

Page 37: ...lot for an injection tool ATC Station with three slots for the tools with the possibility to change an injection tool automatically for specific needs Washing Stations Accommodate the vials of solvents and a waste vial or a drain tube to collect the solvents in a waste container Four types of washing stations are available Standard Wash Station 5 x 10 mL vial Large Volume Wash Station 2 x 100 mL r...

Page 38: ...action technique is used It extend the range of headspace techniques MHE Station Required when the Multiple Headspace Extraction MHE technique is used Fiber Conditioning Station Allows the conditioning of the SPME fiber Bar Code Reader Reads the bar codes located on the vials and sends the content during the report printout Dilutor Module Used for a typical solvent handling and for sampling an exa...

Page 39: ...Phase Micro Extraction on page 14 TriPlus RSH for ITEX In tube Extraction on page 15 TriPlus RSH for Liquids This version features the following characteristics Injectors into which the sample may be introduced S SL PTV PKD PPKD and OCI Washing Station Standard Wash Station Large Solvent Wash Station Large Solvent Station Fast Wash Station Sample Tray 15 position tray Tray 15 32 position tray Tray...

Page 40: ...th every injector port Syringe temperature control from 40 to 150 C Operating modes Constant with or without enrichment Progressive with or without enrichment Multiple Headspace Extraction MHE Constant DoublePro TriPlus RSH for SPME Solid Phase Micro Extraction This version features the following characteristics Injectors into which the sample may be introduced S SL and PTV Washing Station Two via...

Page 41: ...may be introduced S SL PTV PKD and PPKD ITEX2 Tool Washing Station Two vials and four vials Sampler Tray 60 position tray Sample Vial 2 10 and 20 mL Incubation Oven Agitator capacity 6 vials Incubation Oven Agitator temperature control from 40 C to 200 C Syringe capacity 1 2 5 and 5 mL Syringe Needle Length 65 mm compatible with every injector port Syringe temperature control from 40 to 150 C Trap...

Page 42: ...sections Front upper section The part of the crossrail on which the crossrail Y slides Lower section Includes the slots to connect the sampler support and the components Back section Includes the Control Interface for the connection of the sampling unit to the power module the GC and the components installed See the section Control Interface on page 33 It includes the inlet port for the connection...

Page 43: ...ead It represents the Z axis of the system It consists of a vertical structure fixed on the crossrail Y which guides the movements along the X and Y axes See Figure 3 Figure 3 Head The front side is provided with a viewing window allowing access to the injection device See Figure 4 Figure 4 Injection Device Head Viewing Window Injection Device Tool ...

Page 44: ...tors housed in the Head See Figure 5 Figure 5 Injection Device Motors TriPlus RSH Tools The TriPlus RSH requires more information and intelligence on the level of tools than just the syringe identification numbers as used with common samplers The capability to use a tool for one task store the tool away and select a second tool for the next task in the cycle sequence necessitates knowledge of the ...

Page 45: ...ance Counter for Plunger movements total number of plunger strokes counts Maintenance Counter Plunger movements of actual syringe strokes or counts The same Tool holder is mechanically interchangeable for all syringes in the same class with a defined glass barrel diameter and needle length The specific attributes of the syringe can be added to the tool at the moment of installation The very first ...

Page 46: ...e 6 LS Tool with Needle Length 57 mm and 85 mm Tool Identification The tools are labeled at the side The code identifies the Tool required for your s needs See the example in Figure 7 Figure 7 Example Tools Identification Label 57 mm Needle Length LS Tool 85 mm Needle Length LS Tool LS Tool HS Tool SPME Tool ...

Page 47: ...amples of Labels Description Label Description LS 10 μL NL57 Liquid Syringe Tool Syringe Volume 10 μL 57 mm Needle Length LS 10 μL NL85 Liquid Syringe Tool Syringe Volume 10 μL 85 mm Needle Length HS 2500 μL NL65 Headspace Tool Syringe Volume 2500 μL 65 mm Needle Length SPME 1 SPME Tool SPME 1 1 10 mm Fiber Length SPME 1 2 20 mm Fiber Length SPME 2 SPME 2 Tool SPME 2 1 10 mm Fiber Length SPME 2 2 ...

Page 48: ... TriPlus RSH User Guide Thermo Scientific The LS Tool is a single block easy to use to install a syringe See Figure 8 Figure 8 LS Tool The syringe flange is supported by an O ring support bearing See Figure 9 Figure 9 Support Bearing Supporting Bearing ...

Page 49: ...direct metal contact See Figure 10 Figure 10 O Ring The Magnet Adapter Ring is used to transport vials from a Tray to the Agitator The lower needle guide has the Stack Drawer Magnet installed fixed with two screws The magnet is required to open and close a stack drawer See Figure 11 Figure 11 LS Tool Magnets Refer also to Syringes for LS Tools on page 24 Second O ring Stack Drawer Magnet Magnet Ad...

Page 50: ...h 250 μL 500 μL 1000 μL volumes 8 mm diameter syringe barrel with 85 mm needle length 250 μL 500 μL 1000 μL volumes 18 mm diameter syringe barrel with 57 mm needle length 10000 μL volume Table 5 LS Tool Injector Compatibility Sheet 1 of 2 Injector SSL injector hot needle Splitless injection 85 mm needle length LS tool Split injection 57 mm needle length LS tool Large Volume splitless 57 mm needle ...

Page 51: ...e is controlled from 40 C up to 150 C in 1 C steps The syringe is flushed with inert gas flow through X Y Z axis Besides the coupling pivots for the needle guide and the tool the purge gas connector is directly installed on the cover plate of the tool The syringe flange is supported by the polymer counterpart to the retaining nut In the heating cylinder there are two O rings which seal the gas flo...

Page 52: ...O ring Figure 13 HS Tool Cut out The Magnet Adapter Ring is used to transport vials from a Tray to the Agitator as an example The lower needle guide has the Stack Drawer Magnet installed and fixed with two screws The magnet is required to open and close a Stack Drawer See Figure 14 Figure 14 HS Tool Magnets Refer to Syringes for HS Tools on page 27 Stack Drawer Magnet Magnet Adapter ...

Page 53: ...nt fiber lengths commercially available 10 mm and 20 mm Both types can be adapted with the SPME Tool The TriPlus RSH System software allows setting the fiber extension length to the necessary distance The SPME Tool is a single block used to install a SPME Holder See Figure 15 Figure 15 SPME 1 and SPME2 Tools Table 6 List of Syringes for HS Tool Syringe For gas tight syringe of 1 0 mL volume and 65...

Page 54: ...Figure 16 Figure 16 SPME Tool Magnets The Magnet Adapter Ring is used to transport vials from a Tray to the Agitator as an example The lower needle guide has the Stack Drawer Magnet installed and fixed with two screws The magnet is required to open and close a Temperature Controlled Drawer See the section Syringes for SPME Tools on page 29 Stack Drawer Magnet Magnet Adapter Adapter for SPME Condit...

Page 55: ...the total length including the Fiber length See Figure 17 Figure 17 SPME Penetration Depth Parameter Comparison This change in the definition is valid for the SPME Penetration Depth for the following classes Vial Injector SPME Conditioning Module As a result this change in definition affects several method parameters for existing methods When working with RSH Firmware version 1 6 0 to achieve the ...

Page 56: ...nge Following this loading procedure analytes are thermally desorbed and led directly into the GC injector then analyzed by the required technique mainly using GC MS detection Loading takes place by continuously moving the headspace syringe plunger up and down as a pump passing the headspace gas phase that exists above a sample through the sorbent trap inside the needle numerous times The cycle of...

Page 57: ...ce these O rings during preventative maintenance Figure 19 shows the cut out of the ITEX tool visualizing the Purge Gas Flow and the Sealing O ring Figure 19 ITEX Tool Cut out 1 The heating and cooling functionality for the Trap is integrated in the lower part of the tool See Figure 20 Figure 20 ITEX Tool Cut out 2 del gas di lavaggio WARNING BURN HAZARD The ITEX Tool can operate at a maximum temp...

Page 58: ...ed in the ITEX Tool Purge Gas Adapter See Figure 21 Figure 21 ITEX Tool Purge Gas Adapter The Magnet Adapter Ring is used to transport vials from a Tray to the Agitator as an example The lower needle guide has the Stack Drawer Magnet installed and fixed with two screws The magnet is required to open and close a Stack Drawer See Figure 22 Figure 22 ITEX Tool Magnets ITEX Tool Purge Gas Adapter Stac...

Page 59: ...s on page 77 and to the section System Status LED at Status Bar or at X Axis Side Cover in the chapter Troubleshooting of the TriPlus RSH Hardware Manual Table 7 Status LED Lights Sheet 1 of 2 Symbols Description Green status light blinking TriPlus RSH is working moving Execution mode The Handheld Controller is partially blocked to specific actions Green status light steady on TriPlus RSH is ready...

Page 60: ...ion to the CPU board and the components installed on the sampler INTERFACE DSB 15 pin female connector for the connection between the sampler and the GC FUSE Housing for the fuse T6 3 250 VDC See Table 8 POWER KYon KPJX 3S S connector receives 36 V from the Power Module Blue status light blinking The TriPlus RSH is in the process of software Update or Backup For the processes Copy Backup and Resto...

Page 61: ...o the processor via the BUS using the X motor Servo motor X axis Another example is an Agitator where the temperature and motor for sample agitation have to be controlled and monitored A Passive module is a static device Typical examples are non temperature programmable Tray Holders a TR Station or a Standard Wash Station You can easily distinguish the difference between active and passive devices...

Page 62: ...sitions A module ID is automatically provided and stored as long as the device is in operation Loading or activating a specific device firmware is not possible for a Passive module A passive module cannot be deactivated but it can be deleted The module ID will not be stored If the same device is re activated later another module ID will be randomly provided Active Versus Composite Module The term ...

Page 63: ...odules TR Station on page 38 ATC Station on page 39 Standard Wash Station on page 40 Large Volume Wash Station on page 41 Large Solvent Station on page 41 Fast Wash Station on page 42 Agitator on page 43 Vortexer on page 45 MHE Station on page 47 SPME Conditioning Station on page 61 Barcode Reader on page 63 Liquid Cooled Tray Holder on page 69 Temperature Controlled Drawer on page 70 Electronic B...

Page 64: ...our TriPlus RSH TR Station 38 TriPlus RSH User Guide Thermo Scientific TR Station This passive module contains a single slot for an injection tool See Figure 25 and Figure 26 Figure 25 TR Station Figure 26 TR Station with Tool ...

Page 65: ...mo Scientific TriPlus RSH User Guide 39 ATC Station This active module containing three slots for tools allows you to change an injection tool automatically for specific needs See Figure 27 and Figure 28 Figure 27 ATC Station Figure 28 ATC Station with Tools ...

Page 66: ...he Standard Wash Station can be flexible Options include 2 x Wash Vials and 2 x Waste Vials and a Vial dedicated for Internal Standard Reagent and so on 4 x Wash Vials and 1 x Waste Vial This configuration allows using four different Wash Solvents for critical applications When the size of the Waste Vial becomes a problem replace the Vial in the waste position with a drainage adapter Note The sing...

Page 67: ...ume Wash Station The Large Volume Wash Station is shown in Figure 30 Figure 30 Large Volume Wash Station Contains two 100 mL vials and one vial for waste port for diverting waste to large reservoir Large Solvent Station The Large Solvent Station is shown in Figure 31 Figure 31 Solvents Station Contains three 100 mL vials ...

Page 68: ...ring this process The dispensed solvent passes from the overfilled liner into an internal flow channel to the waste outlet This wash process can be repeated The wash solvent can also be changed by moving the syringe to the second wash port and starting the wash process over again The advantage of this station is that the head does not need to move to the waste position to eject the wash solvent Th...

Page 69: ...mical derivatization reactions liquid liquid extractions and so on Temperature and shaking parameters can be individually controlled Figure 33 Agitator Module Solvent reservoir kit 1 30 2 87 Including two 1000 mL bottles Mass Dimension in kg lbs Fast Wash Station 0 750 1 65 Table 10 Operating and Environmental Requirements Parameter Requirements Operating temperature range 4 to 40 C Maximum relati...

Page 70: ...erating temperature and there is a danger of burns WARNING When sample vials have to undergo heating and agitation it is important to consider the glass quality Use high quality glass only Remember that depending on the application conditions high pressure can build up in the vial Whenever a temperature greater than 60 C is applied consider the vapor pressure of the solvent used to ensure that no ...

Page 71: ... extraction of two immiscible solvent phases An emulsion is temporarily formed which will break after the shaking stops Only one vial at time can be treated Figure 34 Vortexer Module Shaking speed can be selected The temperature control of the module is not possible Table 12 Agitator Operating and Environmental Requirements Parameter Requirements Operating temperature range 40 to 200 C Maximum rel...

Page 72: ...y glass vials from reliable suppliers only Do not wash and recycle used vials there is a danger of micro cracks present in the glass It is mandatory using the mixer with the installed protective cover During the mixing step it is important to keep the TriPlus RSH Head always above the vial to avoid an uplift of the vial caused by the spin Use only one vial at time for mixing The recommended applic...

Page 73: ...Principles of Multiple Headspace Extraction MHE on page 49 for details The MHE station consists of a venting tool MHE Adapter combined with a MHE Adapter Holder The MHE technique requires a series of sampling cycles using the same vial First the vial is pressurized to reach a specific pressure The vial is then sampled and vented The vial is re pressurized and sampled again This process is repeated...

Page 74: ...zardous or toxic gases The pressure setting for the MHE technique is 1 0 to 1 5 bar about 15 psi If your system was previously used for static headspace applications you may need to adjust your pressure regulator Table 15 MHE Adapter Dimensions and Mass Length Dimension in mm in Width 25 1 0 Height 51 2 0 Mass Dimension in kg lbs MHE Adapter 0 027 0 06 without vent tubing Table 16 MHE Adapter Hold...

Page 75: ...tion MHE is partially self descriptive To determine the quantity of an analyte sampling is carried out by repeated extraction of gas from the same vial above a sample solution containing the analyte known as the headspace The method thus approaches continuous gas extraction but is carried out in a stepwise fashion In concept the procedure is analogous to the multiple extraction of a sample from a ...

Page 76: ...usually using Helium gas which is also often the carrier gas The pressure is selected so that it is always greater than the pressure increase due to sample heating and vapor pressure of the sample The sample is introduced onto the GC column for analysis and the system is ready for the next sampling after cleaning In MHE equilibrium must be reestablished in the headspace following each extraction W...

Page 77: ...d to be a 50 50 distribution In the course of each extraction all of the headspace is removed exchanged The measured area of the analyte peak is A A ref Area we would measure if all of the analyte were in the headspace corresponding to the measured signal from a similar gas standard A 0 Measured area of the analyte peak from the first extraction With 50 of the analyte present in the headspace this...

Page 78: ...headspace plays no role in the amount of analyte content that is determined from an MHE series Furthermore it can be concluded that quantification based on a single determination from a gas standard will in this particular case yield the correct result assuming that the entire headspace is removed exchanged during each extraction step Situation 3 The equilibrium between the condensed phase and the...

Page 79: ...ion is given by A tot β A ref 1 1 α β 1 α β 2 Using the expression for the geometric sum yields A tot β A ref 1 1 α β β A ref 1 α β or A tot A ref α That is the total area A tot will be over determined by a factor of 1 α relative to A ref In other words the content of analyte will be over determined by the factor 1 α Thus the total area will depend on the amount of headspace removed during each su...

Page 80: ...nto the vial The purge gas is activated for a fixed time of 30 seconds See B in Figure 36 3 The Headspace Syringe Needle is withdrawn from the vial The gas purge valve is shut off after withdrawal See C in Figure 36 4 A sample aliquot is taken up into the syringe according to the sample volume defined in the sample list See D in Figure 36 5 The sample aliquot is injected into the GC See E in Figur...

Page 81: ...1 Getting Familiar with Your TriPlus RSH MHE Station Thermo Scientific TriPlus RSH User Guide 55 Figure 36 MHE Cycle Steps A D E F G H B C ...

Page 82: ...titation based on direct comparison to the response from gas standards The drawback of this procedure is that it neglects any sample with matrix interaction This can lead to the analyte not residing entirely in the gas phase the headspace in its distribution between the condensed phase and the headspace In this case the analyte amount will be underestimated In other words the determined amount of ...

Page 83: ...ent of the analyte is calculated from this value and the calibration curve See D Calculation of the Analyte Content in Real Samples on page 60 A Performing the actual MHE Measurements The parameters of the actual MHE measurements such as incubation time incubation temperature injection parameters and so on can be controlled using the MHE cycle that is part of the MHE kit B Determination of the Tot...

Page 84: ...n overview of each of the steps involved in completing and interpreting the data shown the Excel spreadsheet of Figure 37 Step 1 The measured analyte area corresponding to each of the extraction steps is typed here See the orange field Area in Figure 38 Excel calculates the natural logarithm of the areas for the preparation of the linear regression step See Step 3 ...

Page 85: ...k See Figure 39 Figure 39 Linear Regression for Calibration Standard Analyte Step 3 The built in Excel function for linear regression LINEST is used for the calculation of the parameters of the linear regression fit of the area data The LINEST function is used in matrix mode Figure 40 shows the example of the slope and intercept parameters used in the subsequent calculation Calculation of Linear R...

Page 86: ...d Analyte C Construction of the Calibration Curve For each of the calibration samples the resulting total area determined according to the description above is plotted against the amount of analyte In the following example we have three concentration levels of standards each determined twice thus the calibration curve comprises six points in total See Figure 42 Figure 42 Calibration Curve Total Ar...

Page 87: ...sed for the SPME Solid Phase Micro Extraction technique See Figure 44 It conditions or cleans a fiber between chromatographic runs A flow of inert gas protects the fiber from degradation when it is exposed to elevated temperatures The temperature for conditioning or cleaning can be selected Figure 44 SPME Conditioning Station A spare fiber can be conditioned in the front well to ensure that a spar...

Page 88: ...he gas flow in the channel for the automated conditioning This could result in damage to the fiber as it is not be protected by inert gas The SPME Conditioning Station specifications are Temperature Control From 30 C to 350 C selectable in 1 C increments Electrical Connectors Two BUS connectors for daisy chaining The SPME Conditioning Station dimensions mass operating and environmental requirement...

Page 89: ...rs are activated one after another If the first scanner can read and decode the barcode symbols the second scanner is not activated If the first one fails the second scanner is activated and if the barcode is positively interpreted the process is ended When both scanners cannot read the barcode an error exception is run The error handling itself is part of the CDS software and in most cases you ca...

Page 90: ... of the laser aperture Figure 47 Laser Warning Label and Laser Aperture The selected Laser Class 1 for the module Barcode Reader complies with the following regulations 21 CFR1040 10 and 1040 11 except for deviations pursuant to Laser Notice No 50 dated July 26 2001 EN60825 1 1994 A1 2002 A2 2001 IEC60825 1 1993 A1 1997 A2 2001 The software contains a built in safety time limit such that the laser...

Page 91: ...Universal Product Code 12 numerical digits 11 usable digits 1 check digit 12 12 UPC E Universal Product Code Zero compressed UPC code 7 numerical digits 6 usable digits 1 check digit 6 6 EAN 8 Derived from the longer European Article Number EAN 13 8 numerical digits 7 usable digits 1 check digit 7 7 EAN 13 European International Article Number 13 numerical digits 12 usable digits 1 check digit 12 ...

Page 92: ...he barcodes Barcode Label Positioning Place the barcode label on the vial such that the barcode bars are horizontally positioned Maximum tilt of the barcode label 20 Label Width The barcode label width length of the bars spaces has to be at least 18mm Minimal barcode density minimal width of a bare or a space 5 mil 0 005 0 127mm The allowed area for the placing of the label is given in Figure 48 C...

Page 93: ...dimensional Barcodes The scan engine used for the Barcode Reader Module is designed as a one dimensional 1D scanner Two dimensional barcodes 2D cannot be decoded Barcode Label Size The minimum length of the barcode is 18 mm if used with a 20 mL Vial Please note that this refers to the actual barcode length and not the label itself Barcode Reader Specifications The Barcode Reader Specifications are...

Page 94: ...ltaneously installed each compatible with Three sample tray VT15 VT54 VT70 One sample tray R60 Table 21 Barcode Reader Dimensions and Mass Length Dimension in mm in Width 30 1 18 Depth 273 10 75 Height 128 5 04 Mass Dimension in kg lbs Barcode Reader Module with cable 0 83 1 83 Table 22 Barcode Reader Operating and Environmental Requirements Parameter Requirements Maximum relative humidity 80 non ...

Page 95: ...y Holder is a passive module for an external re circulating bath See Figure 50 Figure 50 Liquid Cooled Tray Holder In the bottom section of the Tray Holder there is an open tube The in hose connections for this tube can be seen at the back of the unit Liquid from an external bath is circulated into the Tray Cooler through this tube the liquid can be either heated or cooled Table 23 Standard Tray H...

Page 96: ...rolled Drawer The Temperature Controlled Drawer is a temperature controlled sample tray that keeps the analytical sample below or above ambient temperature See Figure 51 It is an active module with one drawer two slots and heated or cooled with a Peltier device Includes a separated power supply CAUTION The Liquid Cooled Tray Holder operates within a temperature range from 4 C to 70 C Table 24 Liqu...

Page 97: ...ed for the TriPlus RSH See the chapter Installation of the TriPlus RSH System in the TriPlus RSH Hardware Manual for details Note An Opening drawer status indicates an undefined status or could also signal a defective PCB or sensor Check whether the status changes after manually opening and closing the drawer Drawer Power Module Drawer Closed Drawer Opened CAUTION This module can adapt to Racks ma...

Page 98: ...ed Drawer is rarely used to store a sample at a given temperature for prolonged periods The displayed temperature represents the temperature in the compartment and not in the sample liquid To verify the temperature independently insert a temperature probe at the front of the compartment by removing one plastic screw from the front cover Position the probe in the middle of the drawer where the Tray...

Page 99: ...s not necessary under normal conditions Safety Warning If a purge gas is used it is your responsibility to ensure that a two stage safety pressure regulating device is installed between the gas supply and the Temperature Controlled Drawer If the Temperature Controlled Drawer is operated under severe conditions a flow of clean and dry oil free air or nitrogen could be used to dry the compartments c...

Page 100: ...r temperature cooling is defined as TΔ 14 0 C within 60 minutes TΔ T Room temperature T Temperature Controlled Drawer Electrical Connectors Two BUS connectors for daisy chaining and a connector for external power supply The Temperature Controlled Drawer dimensions mass operating and environmental requirements are detailed in Table 26 and Table 27 Table 26 Temperature Controlled Drawer Dimensions a...

Page 101: ...ure 53 OC Electronic Box The electronic module is laid out to activate the OC Actuator A specification is not applicable The Electronic Box for OC Injector Actuator dimensions mass operating and environmental requirements are detailed in Table 28 and Table 29 Table 27 Temperature Controlled Drawer Operating and Environmental Requirements Parameter Requirements Operating temperature range 4 to 40 C...

Page 102: ...e selected section the status and time are displayed The Content Area shows the list of selected items The Function Keys are located in the Menu Bar See Figure 54 Figure 54 Main Screen Height 160 6 30 Mass Dimension in kg lbs OC Electronic Box with cable 1 08 2 38 Table 29 Electronic Box for OC Injector Actuator Operating and Environmental Requirements Parameter Requirements Maximum relative humid...

Page 103: ...stem Examples Bent needle mechanical problem missing Tool and so on detected at the moment of sample execution A software run time error can also cause a status light change to steady on yellow Blue status light blinking The TriPlus RSH is in the process of software Update or Backup For the processes Copy Backup and Restore the Status light remains green Blue status light permanent TriPlus RSH is ...

Page 104: ...ty at the first possibility The behavior is closer to stop the run but the stop itself occurs after an activity has completed its tasks for example the filling of a syringe Flat Wrench is the indicator for the access level Service User Table 30 Menu Screen Status Bar Symbols Sheet 2 of 2 Symbols Description Note The term Stop usually relates to the controlled end of a process for example finishing...

Page 105: ...ller is still available as an option Figure 56 Handheld Controller and Virtual Handheld Controller The functionality of both the hardware Handheld Controller and the Virtual Handheld Controller software are basically the same Menus selection of modules definition of attributes or feeding parameter values are identical The only difference is the handling When using the Handheld Controller use the S...

Page 106: ...1 Getting Familiar with Your TriPlus RSH Handheld Controller 80 TriPlus RSH User Guide Thermo Scientific Figure 57 Example Context Menu to Enter a Password ...

Page 107: ...the TriPlus RSH and for using the Handheld Controller The use of the Handheld Controller is similar to the Virtual Handheld Controller and the PC software based Handheld Controller Contents Starting the TriPlus RSH Access Levels User Level Extended User Level Service Level Setup Installation and Maintenance Menu Items Help Menu Item About Menu Item Shutdown Menu Item Operation of the TriPlus Syste...

Page 108: ...ng the start up process the start screen shows a blinking Envelope in the Status Line as an indicator of a Pending Message a Service Issue or both See Figure 59 Figure 59 Indicating Messages IMPORTANT Before starting the TriPlus RSH make sure that the sampler and all the modules are properly already installed A syringe of the appropriate type or several syringes Tools named using the appropriate M...

Page 109: ...mple shows that the reconnected Agitator has not been taught yet See the example in Figure 60 Figure 60 Example of Agitator Missing in Extended User Level 2 To retrieve the message proceed as follows a Change the Access Level to Extended User b Press the Options key to access the pull up menu for further information c Select the menu item Pending Message to receive detailed information on the erro...

Page 110: ...e cursor on the list item at the start screen for which you wish to check certain parameters 2 Press the Enter button to open the dialog for the selected item 3 Selecting the Options pull up menu it provides a list of specific tasks limited to the typical needs for routine work The example in Figure 61 shows the resulting errors when the Vortexer cable is not connected and the homing could not be ...

Page 111: ...SH Accessing the Service Level requires a software key which is only provided for trained and authorized service technicians For details see the following sections User Level on page 85 Extended User Level on page 86 Service Level on page 87 User Level The User Level is the normal working level recommended for routine use This level is assigned as the default level The prerequisites for operating ...

Page 112: ... be used for new modules X Y Z axes position teaching changing a sync signal and so on It is highly recommended to operate the TriPlus RSH in the User Level and only for special cases to activate the Extended User Level The Extended User access level provides the experienced operator more possibilities to interact with the TriPlus RSH compared to the routine User Level The distinction between the ...

Page 113: ...ing a device active module To open the Service access level a software key is necessary This key is provided on a Service USB stick which is handed over to trained service engineers The various menu items accessible with Service rights are described in the chapter Setup Menu Item on page 93 Changing Access Level Using the Virtual Handheld Controller the Access levels can be changed by pressing the...

Page 114: ...ress Enter to activate it The asterisk indicates the current active level 2 When the Extended User Access Level is selected a password can be set See Figure 66 Figure 66 Selecting Extended User Level and Setting a Password 3 Select Set Password from the pull up menu a Place the cursor on the first digit and press the Enter button A pop up menu opens providing numerals from 0 to 9 for selection b A...

Page 115: ...zed service technicians only Example of Differences in Access Levels The main difference between the two access levels is that the menu item Service is not available in the User level See Figure 68 Note In case a password is forgotten restore the RSH Firmware See the chapter RSH Firmware in the TriPlus RSH Hardware Manual Use a backup file which does not yet contain the password Be aware that chan...

Page 116: ...owing chapters Chapter 3 Setup Menu Item Chapter 4 Maintenance Menu Items Chapter 5 Service Menu Item Help Menu Item Figure 69 shows the rudimentary Handheld Controller functions Note If the Handheld Controller or the Virtual Handheld Controller is not in use a time out function begins and after 30 minutes of inactivity the software reverts automatically to the default User Level Please note that ...

Page 117: ...em Help About Menu Item This item informs you on which software versions are currently installed with the particular TriPlus RSH See Figure 70 Figure 70 Menu Item About Shutdown Menu Item The menu item Shutdown gives the operator the possibility to shut down the TriPlus RSH in a controlled manner See Figure 71 ...

Page 118: ...in this position In case a TR Station is part of the TriPlus RSH configuration the mead moves to the single slot The software shuts down closes the application and disables the axes After the TriPlus RSH has completed the shutdown procedure it is necessary to turn off the power at the power supply switch at power To restart the TriPlus RSH after the shutdown process toggle the switch of the power ...

Page 119: ... with the Virtual Handheld Controller or directly using the installed Handheld Controller Contents Starting Setup Menu Item Setup Menu Item Modules Setup Menu Item Set Date and Time Setup Menu Item Set TriPlus RSH Name Setup Menu Item Setup Network Note Items highlighted in green are only visible in the access level Extended User ...

Page 120: ...onality in Setup than in the restricted User Level The menu items are listed in alphabetical order Press the Options button to open the pull up menu and select the menu item Setup See Figure 72 Figure 72 Start Screen Options Pull up Menu Refer to the following Setup Items Setup Menu Item Modules on page 95 Setup Menu Item Set Date and Time on page 176 Setup Menu Item Set TriPlus RSH Name on page 1...

Page 121: ... Item Modules Thermo Scientific TriPlus RSH User Guide 95 Setup Menu Item Modules Select the menu item modules to open the list of all installed active and passive modules See Figure 73 Figure 73 List of Active and Passive Modules ...

Page 122: ... page 110 Motors on page 113 Obstacle on page 116 Sample Drawers on page 118 Tool Stations on page 121 TR Station on page 122 ATC Station on page 122 Signals on page 124 Solvent Stations on page 127 SPME Conditioning Station on page 129 Syringe Types on page 131 Tool Adapters on page 134 Tools on page 135 Tray Holders on page 142 Tray Types on page 146 Trays on page 158 Vial Types on page 160 Vial...

Page 123: ...s parameter is often defined by the specific needs for the application Min Temperature Sets the minimal temperature limit Standby Temperature The Standby Temperature can be adjusted to the application s requirements Setting the Standby Temperature using this function does not yet activate the newly selected temperature To activate it go to the pull up menu Options and select Activate Standby Tempe...

Page 124: ...ed Not specified Standard The lid must be opened for access Perforated Syringe or SPME needle can be inserted through the holes in the lid Actual Position Reads out the position of the Agitator Read only function Stdby Temp Control If switched off the module will not go to standby temperature if idle Temp Gain The coefficient used if a temperature calibration has been performed Possible values are...

Page 125: ...evel Setup Module Allows the selection of the Cover Plate Type to reserve one or more vials as a source vial e g for a reagent and to define the Vial Type for the 6 positions See the section List Items Agitator Reserved Positions and Vial Type on page 100 Teach Module The positions of the X Y and Z axes can be defined again teaching Activate Standby Temperature Activates the newly set Standby Temp...

Page 126: ...ial size can be selected for the reserved vials The consequence is that during routine applications the vial overlapping will be possible only within the free available sample vial positions to optimize the throughput The behavior of the following two Activities is summarized Transport Vial activity Allowed for available free sample vial positions and for reserved vial positions Move To Object act...

Page 127: ...tup Menu Item Modules Thermo Scientific TriPlus RSH User Guide 101 Vortexer Upon selecting the list item Agitator Vortexer the following parameters are available for modification See Figure 75 Figure 75 Vortexer Parameters ...

Page 128: ...e defined correctly In case a teaching point is off return to the Menu Setup for readjustment of the teaching position Please note that this action can be done under the Extended User Level but not under User Level Teach Module The positions of the X Y Z axes can be defined again for teaching Enable Motor Enables the motor of the vortexer Disable Motor Disables the motor of the vortexer Check Vort...

Page 129: ...ee Table 36 Table 35 Barcode Reader Parameters Item Description Scanner Select Scanner 1 or 2 Beep After Decode A beep tone can be activated after positive decoding of the barcode Table 36 Options Menu Item Check Teaching Sheet 1 of 2 Item Description Check Teaching Checks the teaching position of the module This task serves as a quick check to determine if the X Y and Z positions are defined corr...

Page 130: ... and the external device See also the section Input Output Interface on page 106 and the section Signals on page 124 Upon selecting the list item Chromatograph System the following parameters are available for modification See Figure 77 GC1 Name for GC System No 1 GC Class description GC System Figure 77 Chromatography System Selection and Parameters Press Enter to edit a parameter See Table 37 Te...

Page 131: ...utput Signal is required Ready A ready signal from another device is expected as an Input Signal Note The synchronization between the TriPlus RSH and any chromatographic system has to be checked for each particular system configuration The setting of the synchronization signal has to be matched with the pin allocation of the synchronization cable The original cable are labeled Check the labels fir...

Page 132: ...he list item I O Interface the following parameters are available for modification See Table 39 Table 39 List of Items for Input and Output Signals Sheet 1 of 3 Item Description Immediate Ignores any Input or Output Signal Signal On Debounce Time Opto In1 Opto isolator or Opto coupler Signal On Debounce Time Negative Logic PWM Out Pulse Width Modulation Signal On Output Voltage Reset Button Reset ...

Page 133: ...long the signal is applied Low Active A standby status of a signal from the chromatographic system can be High Active or Low Active Depending on what has to be applied for the specific system one has to match the signal logic on the TriPlus RSH side By setting a mark to Low Active means that Low Active is selected Low Active disabled means High Active selected Information for the required signal l...

Page 134: ... Table 39 List of Items for Input and Output Signals Sheet 3 of 3 Item Description Table 40 Option Pull up Menu for Input Output Interface Item Description Reset to Default Resets the selected module parameter to default value Beep Actives a Beep sound local on TriPlus RSH If activated the operator can select the Duration Frequency and the Volume of the tonal sound Duration The time for the tonal ...

Page 135: ...epth into the injector Min Needle Penetration Depth Sets the minimum limit of Needle Penetration Depth into the injector WARNING This parameter is used for SPME 1 and SPME 2 Tools only This parameter is not used for LS HS or ITEX Tools The default value is 18 mm The adjustable range is from 10 mm to 50 mm Position Tolerance Checks the difference between the taught X Y Axes positions against the ac...

Page 136: ...dicates a relative detection mode is triggered and the sensor from the Head needle guide is active If the value of this item is set to 0 the sensor of the needle guide is turned off and the syringe slider moves to an absolute value the Z axis position as specified for the object Table 41 Injector Parameters Sheet 2 of 2 Item Description Table 42 Option Menu for Injectors Item Description Reset to ...

Page 137: ...e gas lock is opened or closed If marked as true check box marked the injector is defined as open This can be verified by inserting a syringe manually and if a resistance is noted the gas lock is closed If a mismatch is observed check the settings of the parameters Homing Direction and Homing Position Actual Position Reads out the position of the OC Actuator This information can be used for troubl...

Page 138: ...lt Resets the selected module parameter to default value Check Teaching Checks the teaching position of the module This task serves as a quick check to determine if the X Y and Z positions are defined correctly In case a teaching point is incorrect return to the Menu Setup for readjustment of the teaching position Please note that this action can be done under the Extended User Level but not under...

Page 139: ... Plunger Motor Motor W Actual position Reads only for information Check Plunger Checks the presence of the plunger Tool Control Shows which tool is assigned and whether it is attached to the head See the section Tools on page 135 X Motor Actual position Reads only for information Length X axis Selects the length of the 850 mm or 1205 mm X axis Y Motor Actual position Reads only for information Z M...

Page 140: ...to switch the strategy to Safe mode Safe The Y axis moves from any point first to zero Y back to the X axis before the X movement is started This mode allows bypassing of an object such as a large GC detector that would be directly in the travel path if the Parallel mode is used Default Waste Allows selecting the corresponding Wash Module installed None Important A Waste Module must be defined def...

Page 141: ...ion and references all axes Disable RSH Head All axes are electrically isolated enabling the operator to move the axes manually Enable RSH Head The axes motors are reconnected to mains again Move to Home Moves the TriPlus RSH Head back to Home position without referencing the axes Select Tool Utility Visible only when TR Station is installed Picks the tool from the TR Station Purge Syringe Visible...

Page 142: ...figure SPME Tool Visible only if SPME Tool is installed Configures the SPME Tool with Fiber adjustments Home Motor Allows the Home position of the selected motor Enable Motor Allows enabling the selected motor after it has been disabled Disable Motor Allows disabling the selected motor to permit moving the corresponding axis manually Calibrate Tool Allows calibrating the tool installed in the head...

Page 143: ...tem Description Extension X Extension from the center point of the Obstacle in X axis direction Extension Y Extension from the center point of the Obstacle in Y axis direction Extension Z Extension from the center point of the Obstacle in Z axis direction Table 48 Obstacle Option Parameters Sheet 1 of 2 Item Description New Obstacle Creates a new obstacle Copy Obstacle Copies the present obstacle ...

Page 144: ... Setup for readjustment of the teaching position Please note that this action can be done under the Extended User Level but not under User Level Teach Module The positions of the X Y and Z axes can be defined again teaching Table 48 Obstacle Option Parameters Sheet 2 of 2 Item Description Note The Temperature Controlled Drawer can be adjusted through the BUS protocol For the power supply it is man...

Page 145: ...ture is typically around ambient temperature this helps to dry out the internal parts of a Temperature Controlled Drawer thus eliminating condensation Temp Setpoint Shows the actual target temperature Read only CloseSpeed The speed for closing the drawer can be adapted to a value between 15 and 100 The default value is 80 OpenSpeed The speed for opening the drawer can be adapted to a value between...

Page 146: ...is is a read only parameter The parameter can been in the class Vial Type Rack Type Defines the pattern and sampling sequences of sample locations within a Rack Well Plate Type Defines the pattern and sampling sequences of sample locations within a Well Plate Vial Type Defines the Vial and Cap specifications Table 49 Stack Module Parameters Sheet 3 of 3 Item Description Table 50 Option Pull up Men...

Page 147: ... Needle Penetration Allows adjusting the Needle Penetration Depths in the vial Manually turn the needle as far down in the vial as required The cut out in the Tray Holder frame as well as in the Rack allows visual control of the needle tip Note The value found is not stored automatically This task allows for a check only If the needle penetration should be changed select the Rack Type to enter the...

Page 148: ...New TR Station Creates a new TR Station Copy TR Station1 Copies the present TR Station Delete TR Station Deletes the present TR Station Teach Module The positions of the X Y and Z axes can be defined for teaching Check Teaching Checks the teaching position of the module This task serves as a quick check to determine if the X Y and Z positions are defined correctly In case a teaching point is off r...

Page 149: ...ool is parked in the Slot if not either mounted in the TriPlus RSH Head or not recognized by the system Fiber Protection This parameter allows defining the Slot Position for the SPME Tool Note that this parameter is used for the SPME1 Holder only Table 53 ATC Station Parameters Sheet 2 of 2 Item Description Table 54 Option Pull Menu for ATC Station Item Description Check Teaching Checks the teachi...

Page 150: ...two GC units One signal used could be SWOut1 and the second signal would be SWOut2 The assigned signals have to be matched with the pin allocation of the synchronization cable 2 The second step is to synchronize the signal parameters with the GC This step is made in the section Input Output Interface on page 106 The Ready signal from the GC is usually sent to the system and a TTL In signal is used...

Page 151: ... to program a time delay after receiving the signal usually the Ready Signal from an external device Source Selects the required Input Signal for synchronization with an external device Usually a TTL In contact is used to receive the Ready Signal from an external device Table 56 Output Signal Parameters Sheet 1 of 2 Item Description Output Signal Selects the required Output Signal Signal On Indica...

Page 152: ... require to be in a consecutive order for example Input Signal 2 it can also be a specifically named signal such as Inject or Start Some cycles require a specifically named signal Check the cycle description for specific names Copy Input Signal An Input Signal can be copied with this task The task functions in a similar manner as described above with New Signal Copy Output Signal Copies an Output ...

Page 153: ...Stations opens up the following parameters See Figure 86 Figure 86 Menu Items for Solvent Stations and Menu Item Large Solvent Station Pos1 Large solvent reservoir bottle 100 mL volume in back position Pos2 Large solvent reservoir bottle 100 mL volume in middle position Pos3 Large solvent reservoir bottle 100 mL volume in front position ...

Page 154: ...tion Depth The default value of the syringe needle penetration depth in the reservoir vial is set by this parameter Depenetration Speed Selects the required speed for pulling the syringe needle out of the reservoir vial Diameter Defines the diameter of the solvent reservoir Height Defines the height of the solvent reservoir Penetration Speed The syringe needle penetration speed is set by this valu...

Page 155: ...tation 1 Copies the present Solvent Station Delete Solvent Station 1 Delete the present Solvent Station Check Teaching Checks the teaching position of the module This task serves as a quick check to determine if the X Y and Z positions are defined correctly In case a teaching point is incorrect return to the Menu Setup for readjustment of the teaching position Please note that this action can be d...

Page 156: ...s RSH is powered up In routine use the Standby Temperature is activated after a job has been terminated The Standby Temperature is typically lower than the application temperature Default Needle Penetration Depth Defines the penetration depth of the SPME Needle in the module The value reflects the total length including the length of the fiber Max Needle Penetration Depth Upper limit of penetratio...

Page 157: ...ter Selecting a syringe for example a 10 μL Liquid Syringe opens all the necessary parameters to define the syringe in geometry and to define default values for an optimized use See Table 62 Table 61 Option Pull up Menu for SPME Conditioning Station Item Description Check Teaching Checks the teaching position of the module This task serves as a quick check to determine if the X Y and Z positions a...

Page 158: ...te in μL s Def Fill Strokes Asp Specifies the default fill strokes aspiration flow rate in μL s Def Fill Strokes Disp Specifies the default fill strokes dispense flow rate in μL s Def Fill Strokes Count Specifies the default number of syringe plunger strokes from the same vial to have a homogeneous phase between the headspace gas in the vial and the sample in the syringe Def Fill Strokes Vol Speci...

Page 159: ...enu Options opens up the following tasks See Table 63 Point Style Specify the syringe suppliers specific Scale Length Specify the length of the scale in mm Syringe Volume Specifies the volume of the syringe in μL Gas Inlet Pos Specifies the position in mm of the gas inlet HS Tool only Fiber Length Specifies the length in mm of the SPME fiber Fiber Type Specifies the type of the SPME fiber SPME Too...

Page 160: ... tasks See Table 65 Table 64 MHE Station Adapter Parameters Item Description Z Tolerance A tolerance window to give a plus minus range expressed in mm where the Head must expect an object If the value of this item is 0 this indicates a relative detection mode and the sensor from the Head needle guide is active If the value of this item is set to 0 the Needle Guide Motor detection current is turned...

Page 161: ...ation Deletes of the present MHE Station Check Teaching Checks the teaching position of the station This task serves as a quick check to determine if the X Y and Z positions are defined correctly In case a teaching point is incorrect return to the Menu Setup for readjustment of the teaching position Please note that this action can be done under the Extended User Level but not under User Level Tea...

Page 162: ...enu Item Modules 136 TriPlus RSH User Guide Thermo Scientific Figure 91 Syringe Type Selection for HS LS and SPME Tools Figure 92 Syringe Type Selection for ITEX Tool Press Enter to edit a parameter See Table 66 LS HS SPME ITEX ...

Page 163: ...g this function does not yet activate the newly selected temperature To activate it go to the pull up menu Options and select Set Standby Temperature The Standby Temperature is activated when the TriPlus RSH is powered up In routine use the Standby Temperature is activated after a job has been terminated The Standby Temperature is typically lower than the application temperature Temp Setpoint Read...

Page 164: ...ature limit The Minimum Temperature which can be set is 40 C Standby Temperature The Standby Temperature can be adjusted to the application s requirements Setting the Standby Temperature using this function does not yet activate the newly selected temperature To activate it go to the pull up menu Options and select Set Standby Temperature The Standby Temperature is activated when the TriPlus RSH i...

Page 165: ...tivated after a job has been terminated The Standby Temperature is typically lower than the application temperature Temp Setpoint Read out the setpoint of the temperature Stdby Temp Control If switched off the module will not go to standby temperature if idle Temp Gain The coefficient used if a temperature calibration has been performed Possible values are between 0 and 2 Read only Temp Offset The...

Page 166: ... All syringes with the same geometry barrel diameter needle length and corresponding syringe type to the tool are filtered by the software and are selectable for use Table 66 List Item Parameters for HS LS SPME and ITEX Tools Sheet 4 of 4 Item Description Table 67 Options Parameters for HS LS SPME and ITEX Tools Item Description Calibrate Tool Calibrates the specified tool Note This might be neces...

Page 167: ...n order to transport a vial If a mismatch is observed such as if a 2 mL vial with a magnetic cap is in use but the Needle Guide Type is selected for a 20 mL vial then the software will prohibit the execution of the run Figure 93 illustrates the interdependency between the various software classes and subclasses The different colors depict the main classes and the parameters assigned to each of the...

Page 168: ...tomatically linked to the Needle Guide Type which in turn will determine whether the Foil Cutter will be used The Needle Guide Type is not a software class directly linked to the class Tray Holder and its subclasses rather it is linked to the class Tools The Needle Guide Type will determine which adapter has to be used to handle the selected vial or well plate Is it necessary to transport a vial a...

Page 169: ...lders Press Enter to edit a parameter Selecting the list item Slot opens the dialog window for Tray Rack which contains the parameters Tray Type and Vial Type If no Tray is assigned yet use the button New to assign a Tray to a particular Slot The dialog for naming a Tray can be started by pressing the Enter button See the examples See Figure 95 ...

Page 170: ...lus RSH User Guide Thermo Scientific Figure 95 Tray Type and Vial Type Selections to Assign to Tray Slot Select the required Slot number to assign a Tray Type and Vial Type Tray Select the required Tray Rack number to assign the Tray Type and Vial Type ...

Page 171: ... A passive Tray Holder module can adapt Trays made for different vial sizes When the different Trays are used consider the various vial heights and ensure that the Stack Drawer Magnet connected to the lower needle guide does not catch at front row of vials from behind the Tray Table 68 Tray Holder Option pull up Menu Parameters Item Description Create New Tray System Guides through a wizard for cr...

Page 172: ... well as in the Tray allows visual control of the needle tip Note The value found is not stored automatically This task allows a check only If the needle penetration must be changed select the Tray Type to enter the new established Needle Penetration value Check Teaching Checks the teaching position of the module This task serves as a quick check to determine if the X Y and Z positions are defined...

Page 173: ...nserted the combined height of a Tray and the height of the vial above the upper surface of the Tray yields to the total height of the Tray Module A Plate has no vial inserted and the total height of the Tray corresponds with the height of the Well Plate The second main difference is the order of sample treatment A Tray Type R60 has the vial numbering in the order of the Y axis in a Well Plate the...

Page 174: ...n direction Y axis Unit μm Alt Sample Offset Z Offset from Tray Zero Point to first sample position of staggered row column in direction Z axis Unit μm This parameter is currently not used with released Tray Types Value 0 Cavity Depth Defines the total depth of the cavity Unit mm Cavity Diameter Defines the diameter of the cavity Unit mm Columns Number of columns Indexing Orientation Defines the o...

Page 175: ...ze This parameter is for example used for the Tray Type R60 Value 3 8 mm Slot Span Number of the Tray installed on the Tray Holder Examples Tray 60 Slot Span 1 Tray 54 Slot Span 3 Note A Tray Type with a Slot Span 1 has to be positioned in Slot 1 The Rack does span from Slot 1 to Slot 2 to Slot 3 Total Column Length X Defines the total length of the Column X axis Distance from center to center of ...

Page 176: ... the plate top surface Unit mm DefNdlPenetrDepth Parameter used for MTP only Defines the default value for the syringe needle penetration depth in the well of the Micro Titer Plate MTP Unit mm Depenetration Speed Parameter used for MTP only Defines the speed of the syringe needle de penetration from the well of the Micro Titer Plate MTP Unit mm s Sealing Foil Parameter used for MTP only Setting th...

Page 177: ...olumns Number of Columns Definition of Row and Column Example Regular order of vials Figure 97 Tray type R32 Liquid Cooled Rows Number of Rows Columns Number of Columns Definition of Row and Column Example Regular order of vials Figure 98 Tray type VT54 Rows Number of Rows Columns Number of Columns Definition of Row and Column Example Regular order of wells Figure 99 Tray type MTP 96 ...

Page 178: ...00 Tray type R60 Rows Number of Rows Columns Number of Columns Definition of Row and Column Example Staggered order of vials Figure 101 Tray type R12 Indexing Orientation Direction X or Row Direction with RSH Firmware 1 4 0 or lower Direction Y or Column Direction with RSH Firmware 1 4 0 or lower Figure 102 Indexing orientation Table 71 Tray Type Parameters Sheet 2 of 7 Tray Type Parameter Illustr...

Page 179: ...mple Offset Y 1st Sample Offset Z Figure 103 Tray type R32 LiqCooled 1st Sample Offset X 1st Sample Offset Y 1st Sample Offset Z Figure 104 Tray type VT54 Alt Sample Offset X Alt Sample Offset Y Alt Sample Offset Z Figure 105 Tray type R60 Table 71 Tray Type Parameters Sheet 3 of 7 Tray Type Parameter Illustration ...

Page 180: ...t Y Alt Sample Offset Z Figure 106 Tray type R12 Length X Length Y Length Z Total Column Length X Total Row Length Y Figure 107 Tray type R32 Liquid Cooled Length X Length Y Length Z Total Column Length X Total Row Length Y Figure 108 Tray type VT54 Table 71 Tray Type Parameters Sheet 4 of 7 Tray Type Parameter Illustration ...

Page 181: ... User Guide 155 Cavity Depth Cavity Diameter Figure 109 Tray type MTP96 Penetration Vial Index Figure 110 Tray type VT54 Z Tolerance Figure 111 Tray type R60 Z Tolerance Figure 112 Tray type MTP96 Table 71 Tray Type Parameters Sheet 5 of 7 Tray Type Parameter Illustration ...

Page 182: ...e VT54 inserted Slot Span Example Slot Span 3 Figure 114 Tray Holder with Tray Type R60 inserted Slot Offset X Slot Offset Y Note that the value has a negative sign if the Tray is oversize which means larger than the Tray Holder Figure 115 Tray Holder with Tray Type R60 inserted Table 71 Tray Type Parameters Sheet 6 of 7 Tray Type Parameter Illustration ...

Page 183: ... R60 inserted Uses Adapter Figure 117 Agitator Table 71 Tray Type Parameters Sheet 7 of 7 Tray Type Parameter Illustration Table 72 Tray Types Options Pull up Menu Item Description New Tray Type This task creates a new Tray Type Copy Tray Type This task copies the present Tray Type for example VT54 Delete Tray Type This task deletes the present Tray Type for example VT54 Reset to Default Resets th...

Page 184: ... class Trays Selecting the list item Tray Types opens up the following parameters See Figure 118 Figure 118 Menu Item Trays Menu List Item Trays Press Enter to edit a parameter See Table 73 Trays Options Pull up Menu Selecting the pull up menu Options opens up the following tasks See Table 74 Table 73 Tray Type and Vial Type Parameters Item Description Cap Type Read only parameter Definition is ma...

Page 185: ...her pull up menu is available from the menu list items Tray Type and Vial Type Selecting the item Adjust Needle Penetration allows checking the needle penetration in the vial The position of cut out in the Tray is specified in the Tray Type therefore is the position known Starting this task moves the TriPlus RSH Head to the defined vial position where it waits for the manual adjustments See Figure...

Page 186: ...ble 75 Trays Options Menu Parameters Item Description Adjust Needle Penetration Adjusts the Needle Penetration Depths in the vial Manually turn the needle as far down in the vial as required The cut out in the Tray Holder frame as well as in the Tray allows visual control of the needle tip Note The value found is not stored automatically This task allows a check only If the needle penetration must...

Page 187: ...al Type 100SR Wash Vial and Solvent Reservoir Vial and Waste Vial show identical parameters The sample vial Vial Type 10 CV contains extra parameters Allow Needle Transport Cap Type Dispose In Waste Container Strip Distance Table 76 Vial Types Parameters Sheet 1 of 3 Item Description Default Needle Penetration Depth The default value of the syringe needle penetration depth in the reservoir vial is...

Page 188: ...ve the syringe needle from the vial Diameter Defines the diameter of the reservoir container Dispose In Waste Container Available for Vial Type only Used if e g a pipetting tip or a SPE cartridge has to be expelled into a dedicated Waste Container All parameters as used for liquid disposal such as rinsing syringe and so on are not functional Height Defines the height of the reservoir container Max...

Page 189: ...needle guide is active If the value of this item is set to 0 the sensor of the needle guide is turned off and the syringe slider moves to an absolute value Z axis position as specified in the object IMPORTANT Using the SPME technique pay special attention to the parameters DefNdlPenetrDepth and MaxNdlPenetrDepth The penetration depth is always the penetration of the needle itself Consider the leng...

Page 190: ... dilution vial When the application requires a second Waste Vial or an extra Wash Vial then this class of Vial could be used to define the non standard vial The three categories Wash Vial Waste Vial and Solvent Reservoir contain the same available parameters under the User Access Level Selecting the list item Vial opens up the following parameters Figure 122 Menu List Vials and Menu List Items for...

Page 191: ...gure 123 Menu Button New Vial Selection of Vial Types and Defining Name for New Vial The parameters available for the Vial Type reservoir are slightly different from the parameters for the Wash Vial and Waste Vial See the examples in Figure 124 and Figure 125 and the description in Table 78 ...

Page 192: ...d Needle Penetration can be defined as a safety measure so that you cannot destroy the needle tip by going too far down The maximum needle penetration must not exceed the length of the syringe needle considering the loss of approximately 12 mm due to the needle guide and other factors For a typical needle length of 57 mm or 85 mm for the long needle the maximum penetration depth must not exceed 45...

Page 193: ...pes opens up for selection Special cases for Waste Vial None It is selectable however if selected in combination with a Wash Module is an error message Pending Message propagated Incompatible Type Waste Port Used for the Fast Wash and Large Wash Module Waste Vial Used for Standard Wash Module Waste Inset This type is used for Standard Wash Module with inserted Waste Line Adapter Volume Specific fo...

Page 194: ...Table 79 Options Vial Type Pull up Menu Item Description New Vial Creates of a new Vial Copy Vial Copies the present Vial Delete Vial Deletes the present Vial Reset to Default Resets the selected Vial parameter to default value Check Teaching Checks the teaching position of the module This task serves as a quick check to determine if the X Y and Z positions are defined correctly In case a teaching...

Page 195: ...tions 1 to 4 are available for the Standard Wash Station Each Position can be defined by using the next parameters Waste Defines the Waste position Max Penetration Depth The maximum allowed Needle Penetration can be defined as a safety measure so that the you cannot destroy the needle tip by going too far down The maximum needle penetration must not exceed the length of the syringe needle consider...

Page 196: ...s indicates a relative detection mode and the sensor from the Head needle guide is active If the value of this item is set to 0 the Needle Guide Motor detection current is turned off and the syringe slider moves to an absolute value Z axis position as specified in the object Diameter Specific for Waste Vial Defines the diameter of the reservoir container Height Specific for Waste Vial Defines the ...

Page 197: ... the solvent flow is unrestricted Expect a higher solvent consumption Def Ndl Penetr Depth Sets the default value of the syringe needle penetration depth in the reservoir vial The default values are 25 mm in Wash position 20 mm in Waste position Depenetration Speed Selects the required speed for pulling the syringe needle out of the reservoir vial The default values are 100 mm s in Wash position 1...

Page 198: ...etection current is turned off and the syringe slider moves to an absolute value Z axis position as specified in the object Diameter Specific for Waste Vial Defines the diameter of the reservoir container The default value is 20 mm Height Specific for Waste Vial Defines the height of the reservoir container The default value is 80 mm Vial Type Defines the Vial Type of the reservoir container in Po...

Page 199: ...tions of the X Y and Z axes can be defined again teaching New Wash Station Creates of a new Wash Station Copy Wash Station Copies of the present Wash Station Delete Wash Station Deletes of the present Wash Station Table 83 Fast Wash Option Parameters Item Description Teach Module The positions of the X Y and Z axes can be defined again teaching Reset to Default Resets the selected station paramete...

Page 200: ...fic Tray Slots This menu List Item will be implemented in future See Figure 127 Figure 127 Menu Item Tray Slots Unknown This menu List Item will be implemented in future See Figure 128 Figure 128 Menu Item Unknown Valves This menu List Item will be implemented in future See Figure 129 ...

Page 201: ... Menu Item Modules Thermo Scientific TriPlus RSH User Guide 175 Figure 129 Menu Item Valves The Menu Items which are not part of the system configuration showing 0 behind the class name are not listed in the User access level ...

Page 202: ...r access level only 1 Select in the Option Setup pull up menu the list item Set Date and Time See Figure 130 and Table 84 Figure 130 Set Date and Time Table 84 Date and Time Format Item Description Set Date and Time Date format yyyy mm dd Time format hh mm ss Time Zone Greenwich Mean Time GMT plus or minus the hour deviation from Greenwich standard time ...

Page 203: ...131 Figure 131 Set TriPlus RSH Name 2 Press Enter to receive the alphabetical selection of characters or press the Default Action button to change from capital to lower case characters or to numbers The name has to start with an alphabetical character 3 Set a unique name for the TriPlus RSH This name will be stored and can be used for identification of the system 4 Press Option to receive the list...

Page 204: ... 0 Default Gateway not required leave the field empty if an address is entered it will not be used Corporate Network with DHCP Protocol Ask the network administrator to verify and integrate the setup within the corporate network Select the DHCP Protocol the network will assign an IP Address and a Subnet Mask to the TriPlus RSH Corporate Network with Fixed IP Address Ask the network administrator t...

Page 205: ...Address The IP Address is a fixed system address The IP Address is often provided by the network administrator Default Address TriPlus RSH 192 168 99 230 Subnet Mask The Subnet Mask is used for network configuration it must be provided by the network administrator Default Subnet mask 255 255 255 0 Default Gateway The Gateway is used if independent networks must communicate interactively Communicat...

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Page 207: ... Maintenance Menu operated either with the Virtual Handheld Controller or directly using the installed Handheld Controller Contents Maintenance Menu Check Tool Calibration Check Teaching Module Check Configuration Reset Configuration Create Diagnostic Backup Create Configuration Backup Copy Backup Restore Backup ...

Page 208: ...d select Maintenance 2 Select the folder Maintenance for accessing the list items Check tool Calibrations Check Teaching modules Backup and Restore Refer to the following items Check Tool Calibration on page 183 Check Teaching Module on page 183 Check Configuration on page 184 Reset Configuration on page 184 Create Diagnostic Backup on page 185 Create Configuration Backup on page 186 Copy Backup o...

Page 209: ... TriPlus RSH Check Tool Calibration in the TriPlus RSH Hardware Manual Check Teaching Module Selecting Check Teaching modules the TriPlus RSH Head moves from one module to the next in the order listed in the table modules The unit waits at the programmed teaching point for visual inspection press the button Next to continue In case a teaching point is incorrect return to the Menu Setup for readjus...

Page 210: ...can be retrieved again The software checks the entire configuration for any hidden problems The message symbol envelope in the Status Line indicates that one or more mismatches have been detected Select from the pull up menu the Pending Messages or Service Issues Note that the messages can be opened and viewed in the Extended User access level only Reset Configuration By selecting Reset Configurat...

Page 211: ...allation Wizard again Path from Start Screen Options Service Installation Access level Extended User is required Table 86 List Items for Reset Configuration Item Description Factory Setting 1 S This will set the length of the X Axis to 850 mm Factory Setting 2 L This will set the length of the X Axis to 1205 mm Factory Setting 3 ITSP S This will set the basic configuration for the dedicated applic...

Page 212: ...Base Presets Defines a model configuration such as liquid and headspace injection technique and so on This tool is available in the Extended User and Service access levels After successful creation of the Diagnostic Backup copy the file directly onto the USB Stick The creation of the Diagnostic Backup file with extension pack does take approximately eight minutes The size of the file is in the ran...

Page 213: ...ood practice to store the configuration backup in a safe place if possible on a network server You must keep an authorized configuration backup outside of your premises To copy a configuration backup from the SD card insert the USB stick into the connector USB Host on the back of the TriPlus RSH Refer also to the chapter RSH Firmware in the TriPlus RSH Hardware Manual Note Be aware that only one b...

Page 214: ...file pack file can be used The key the encryption of the Application and the device Firmware files must match Refer also to the chapter RSH Firmware in the TriPlus RSH Hardware Manual Import Templates Selecting the menu item Import Templates allows the Extended User to import one or more customized templates for several iemes sach as Needle Types Rack Items Syringe Types Tray Slot Types Tray Types...

Page 215: ...ns of the Service Menu operated either with the Virtual Handheld Controller or directly using the installed Handheld Controller Contents Service Menu Installation Item Create New Tray System Item Teach Home Position Note Items highlighted in green are only visible in the access level Extended User ...

Page 216: ...default To enable access to the Extended User Level press the two Menu Buttons simultaneously or press the two characters A and B simultaneously on the keyboard if you are working with a Virtual Handheld Controller For more details see the section Access Levels on page 85 Figure 134 Changing Access Level When the TriPlus RSH is powered up the first time the Message Envelope is shown in the Menu Sc...

Page 217: ...n Motors has been completed Each point ticks off as it is completed When the TriPlus RSH is powered on for the very first time these wizard items which have to be accomplished are active black The grayed out wizard items will be active as soon as the system is calibrated Calibrate Motors and if the hardware configuration of the TriPlus RSH requires these points Note During a new installation of th...

Page 218: ...n page 193 Set Date and Time on page 194 Set TriPlus RSH Name on page 195 Setup Network on page 196 Calibrate Motors on page 199 Teach Tool Station on page 202 Configure SPME Tool on page 207 Set Reference Point on page 209 Calibrate Tools on page 210 Setup Module on page 213 Teach Module on page 214 Check Teaching Modules on page 218 Create a Backup File on page 220 ...

Page 219: ...tings Item Description Language English is the default language Regional Setting Corresponds to the country settings of the computer operating system The country setting mainly influences formatting such as for dates and numbers IMPORTANT When a Set Regional Setting parameter is changed a reboot of the system is required Please note that the Regional Settings are active for the Handheld Controller...

Page 220: ...rd item Set Date and Time to adjust the date and time See Figure 138 and Table 89 Figure 138 Wizard Item Set Date and Time Table 89 Set Date and Time Parameters Item Description Date The format is yyyy mm dd Time The format is hh mm ss Time Zone Greenwich Mean Time GMT plus or minus the time deviation in hours from Greenwich standard time ...

Page 221: ... Item Set TriPlus RSH Name 2 Press Enter to receive the alphabetical selection of 15 characters maximum or press the Default Action button to change from capital to lower case characters or to numbers 3 Press Options to access the list items Insert or Delete Character or Clear Text 4 Press Ok when finished Note The name must start with an alphabetical character and end with an alphanumerical chara...

Page 222: ...ress Ok when finished Figure 140 Wizard Item Setup Network Table 90 Set TriPlus RSH Name Parameter Item Description Set TriPlus RSH Name Set a unique name for the TriPlus RSH This name will be stored and is used for identification of the system Insert Character Allows inserting a character Press Enter to receive the selection of capital letters Pressing the Default Action button will switch to low...

Page 223: ...etwork Defines the Network communication parameters DHCP Dynamic Host Configuration Protocol If activated an IP Address is dynamically sought throughout the available network It is advisable not to use the DHCP mode but to use a fixed IP Address IP Address The IP Address is a fixed system address The IP Address is often provided by the network administrator Default address TriPlus RSH is 192 168 9...

Page 224: ... default values For more information refer to Setting Up Network Interface in the TriPlus RSH Hardware Manual In order to select the required network setup according to the laboratory setting the following possibilities are available Direct connection The TriPlus RSH is directly connected to the computer via the LAN cable There is no corporate network or router connection IP Address TriPlus RSH 19...

Page 225: ...ibration of the axes is mandatory and ensures reliable performance This step has been repeated when a motor has failed has been replaced or when any other major error has occurred This calibration task brings the TriPlus RSH back to reliable operation To calibrate motors 1 Select the wizard item Calibrate Motors and follow the dialog In the following example the Calibration Wizard guides you from ...

Page 226: ...hermo Scientific 2 Press Next to continue with the Calibrate Coupling Step 2 3 When finished press Next again to Calibrate Plunger Sensor Steps 3 4 Step 4 calibrates the coupling of a tool The wizard text explains that the head must be moved above the Coupling Pivots ...

Page 227: ...ssfully completed press Next to continue to step 5 to calibrate the coupling 6 In Step 6 the Sensor of the Plunger motor is calibrated When finished press Next to continue Note Please note that the TriPlus RSH Head has to be positioned such that the receiving holes of the Z Slider are above the Coupling Pivots of the tool but they must not be engaged Note When the tool ejection has been completed ...

Page 228: ...ith one or two ATC Stations The following configurations are allowed One TR Station One or two ATC Stations The following configurations are prohibited Note Tool Station is a generic term It can refer to a TR Station passive module with single slot or to a ATC Station active module with three slots This section describes the teaching of a ATC Station The teaching of a TR Station uses the same step...

Page 229: ... software will check if the tool coupled in the TriPlus RSH head is parked in the expected position In case the two ATC Stations are mixed up an error message appears In such a case verify the identify of the ATC Station the slot position or both Wizard Teach Tool Station The wizard procedure is identical for teaching a TR Station or an ATC Station The difference is the number of slots to teach CA...

Page 230: ...42 Wizard Item Teach Tool Station The wizard automatically turns off the current to the TriPlus RSH to enable a manual movement of the TriPlus RSH Injection Unit to the first Slot If you have performed Calibrate Motors on page 199 a tool should still be installed If for any reason there is no installed tool the TriPlus RSH will recognize the situation and will go through an intermediate step to mo...

Page 231: ... When a tool has already been installed the wizard starts with Step 1 See Figure 144 Figure 144 Teach Slot 1 Step 1 asks you to place the TriPlus RSH Injection Unit manually into the first slot Ensure that the guiding pins engage well into the guiding holes of the ATC Station When positioned well press Save to store the X Y and Z Axes positions 4 Repeat the previous steps to teach the positions fo...

Page 232: ...stallation Item 206 TriPlus RSH User Guide Thermo Scientific Figure 145 Teach Slot 2 and Slot 3 5 The last step moves the TriPlus RSH Injection Unit back to the Home Position See Figure 146 Figure 146 Step 4 to finish Wizard ...

Page 233: ...ool manually in a slot 2 Select the previous wizard point Teach Tool Station again Wait until the first movements of the Z Axes are finished 3 Press Back and leave this wizard point without going through all the steps Check if the wizard point Configure SPME Tool is active To configure the SPME Tool 1 Select the wizard item Configure SPME Tool See Figure 147 Figure 147 Wizard Item Configure SPME T...

Page 234: ...lignment 4 Adjust the Standby Position Bring back the fiber by at least 2 mm into the needle protection sheet so the fiber does not touch the septum when penetrating through The fiber could stick to the septum and be damaged or even lost See Figure 149 Figure 149 SPME Fiber Standby Position For the two versions an approximate value for the parameter Fiber Alignment and the recommended value for th...

Page 235: ... hand the Y Axis and with the second hand the tool body to move in a controlled way the TriPlus RSH Injection Unit to the teaching position Do NOT hold the Needle Guide to move the TriPlus RSH Injection Unit This part is mechanically flexible and could lead to misalignment The TR Station or ATC Station should not change in their position and only calibrated tools should be used If the Station has ...

Page 236: ...bration This tool can be considered as the ideal tool All other tools will be referenced with respect to the Ideal tool This step is not necessary when a single tool station the TR Station is used or if only one tool is in use within the entire system CAUTION It is advisable to hold the Y Axis with one hand and with the other hand hold the tool body to move the TriPlus RSH Injection Unit in a cont...

Page 237: ...has to be specified for the application The definition can be made at this stage of installation or later on during routine work You are able to change this parameter to adapt it for another application note that the Extended User Level is required Table 93 Calibrate Tools Parameters Item Description Not Specified Prohibits the execution of a run Magn10or20mL Magnetic Ring installed to enable the ...

Page 238: ...ep of Tool Calibration the wizard will park the Ideal tool and pick up the next tool starting with the tool in Slot 1 and consecutively to Slot 2 and Slot 3 See Figure 154 and Figure 155 Figure 154 Preparing Tool Change for Step 2 Check Tool and Pick HS Tool Figure 155 Tool Change to Prepare for Step 3 and Calibrating HS Tool Note If Needle Guide Type is defined as Not Specified the execution of a...

Page 239: ...re 156 Wizard Item Setup Module To setup a module The example below shows the steps for defining the parameters for the Agitator CAUTION TR Station or ATC Station should not change in the position If the Station has moved its position it is necessary to redefine the Reference Point and recalibrate all the other tools CAUTION If for any reason the referencing Set Reference Point has been repeated i...

Page 240: ...rm Teaching is used for the definition of the X Y and Z Axis positions for each module Refer also to TriPlus RSH Defining Object Positions in the TriPlus RSH Hardware Manual To teach the module 1 Select the wizard item Teach Module A dialog will guide the operator through this teaching wizard Upon starting the wizard the TriPlus RSH automatically turns off the current to enable a manual movement o...

Page 241: ... needle guide perfectly within the center of the lunette ring Without the magnetic ring attached use the inner ring for teaching 5 The Lower Needle Guide should be centered on the lunette with the bottom of the Lower Needle Guide lightly touching its surface For modules without a lunette such as the Agitator it is necessary to insert a vial with the corresponding adapter if required and use the vi...

Page 242: ...ach position of each module is reported in Table 94 Table 94 Teach Modules Steps Sheet 1 of 3 Steps Agitator Teaching Position Vortexer Teaching Position Barcode Reader Teaching Position SPME Conditioning Station Teaching Position GC Injector Teaching Position OC Actuator Teaching Position ...

Page 243: ...r device for example the Thermo Conductivity Detector TCD from a GC system If a module exceed a certain height it could be get in the path way of the TriPlus RSH The dimension and the position of the obstacle can be defined The TriPlus RSH excludes this area from the allowed path way and bypasses it MHE Station Teaching Position Tray Holder 1 Teaching Position Tray Holder 2 Teaching Position Table...

Page 244: ...vious module and verifies the teaching position again Check Teaching Modules This section allows to check and verify the teaching positions of all the modules included into the system Solvent Reservoir Teaching Position Wash Vial Teaching Position Fast Wash Station Teaching Position Large Wash Station Teaching Position Standard Wash Station Teaching Position Teaching Position Table 94 Teach Module...

Page 245: ...me way as it would for a routine run In other words the cover of the Agitator is opened automatically or in combination with the MHE Adapter it moves the TriPlus RSH Head to the side to stripe off the magnetic adapter 4 Press Next to continue within the wizard the TriPlus RSH Head moves to the next module 5 The pull up menu Previous moves one step back to the previous module and verifies the teach...

Page 246: ...dware Manual To create a configuration backup file 1 Select the wizard item Create Configuration Backup and follow the dialog See Figure 159 Figure 159 Wizard Item Create a Backup File 2 The proposed name for the backup file is After Installation The file is tagged with the current date and time The file name can be changed by the operator Press the Enter button to open the alphanumeric characters...

Page 247: ...operator how many percent are completed 4 This process only takes a few minutes After completion press Exit to finish the installation wizard Note The Backup file is stored in the SD card which is installed on the control board This file can be saved on the USB Stick see the chapter Setup Menu Item on page 93 The file can also be used to make a Restore of the Control Software Please note this opti...

Page 248: ...is mainly used if a custom specific Tray Holder will be used To create a new tray system 1 Select the menu item Create New Tray System See Figure 161 Figure 161 Selecting Menu Item Create New Tray System 2 By opening the individual list items pre selected parameters are available for use See Figure 162 Figure 162 Tray Holder Choices Note The Tray can be either a Tray or a Well Plate Accordingly th...

Page 249: ...eate New Tray Type Parameters Item Description Tray Holder Tray Holder Liquid Holder passive module Tray Slot Tray Slot currently the only supported type Tray Tray currently the only supported type Tray Type Tray 15 Tray 54 Tray 32 Tray 60 Tray 70 See the section Definition of Terms Naming Conventions and Start Screen Icons on page 3 for details Vial Type 1 CV 2 CV 10 CV 20 CV Regular Vials 2 SR 1...

Page 250: ... final configuration of each system The Home Position optimization is performed for several reasons as For convenience in order to have the head in a preferred standby position To save time minimizing the movement of the head to a module For avoiding the collision of the head and a module Changing Home Position To change a home position There are two ways to get to the Menu item Teach Home Positio...

Page 251: ...tific TriPlus RSH User Guide 225 3 Under the screen RobotArmLeft select the pull up menu Options Teach Home Position Select 4 Move the head to the required position and save the X Y Z Axes Positions as the new Home Position Press Ok to leave this wizard ...

Page 252: ...5 Service Menu Item Teach Home Position 226 TriPlus RSH User Guide Thermo Scientific ...

Page 253: ...rview of possible causes and recommended actions which can be taken to eliminate an erratic behavior This guide does not replace the troubleshooting guides for chromatographic techniques Contents General Points Regarding Chromatographic Effects Headspace Technique Points Regarding Chromatographic Effects ...

Page 254: ...le penetration into sample vial Sample volume too low Increase sample volume Wrong setting of syringe needle penetration in GC injector Check Needle Penetration Depth settings and compare with GC manufacturer recommended parameters Syringe does not fill properly Air bubbles below syringe plunger It is possible that air bubbles remain below the plunger after the first pull up If the plunger is move...

Page 255: ...se Delay Post Inj Delay Note that for dedicated cycles certain parameters are locked Excessive carryover between samples Dirty syringe Increase Pst InjSlv1 2 values in method Use PreClnSlv1 Slv2 and PrClnSpl Check and if necessary replace vial septa from Wash Waste vials Inappropriate wash solvent Use appropriate wash solvent Wash Solvent and Waste vial septa too tight Check if the syringe fills c...

Page 256: ...neral Points Regarding GC Effects Troubleshooting Guideline Sheet 3 of 3 Symptom or Error Message Possible Cause Recommended Action Table 97 Headspace Technique Points Regarding GC Effects Troubleshooting Guideline Sheet 1 of 2 Symptom or Error Message Possible Cause Recommended Action Sample peaks or responses are not reproducible Dirty syringe Increase method value Syr Purge Syringe pressure dif...

Page 257: ...y parameter such as the injector oven or detector temperature can contribute to bad peak shape Check the column type and connections square cut connections Excessive carry over between samples Dirty syringe Increase method value Syr Purge Set the value of the method parameter Syringe Temp 10 C above the Agitator temperature Incubation Temp None or too low Syringe Purge gas pressure Check pressure ...

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