Thermo Scientific TriPlus RSH, User Manual

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1
Getting Familiar with Your TriPlus RSH
MHE Station
60 TriPlus RSH User Guide Thermo Scientific
Step 4
The total area is the limit value of the geometric sum. The two parameters needed for the
calculation of the sum are directly determined by the linear regression. They are the slope and
the intercept of the regression curve, respectively. The
calculated total area is the result of the
MHE determination. See Figure 41 .
Figure 41. Calculation of Total Area of Calibration Standard Analyte
C. Construction of the Calibration Curve
For each of the calibration samples the resulting total area, (determined according to the
description above), is plotted against the amount of analyte.
In the following example we have three concentration levels of standards, each determined
twice – thus the calibration curve comprises six points in total. See
Figure 42 .
Figure 42. Calibration Curve: Total Area versus Amount of Acetone
D. Calculation of the Analyte Content in Real Samples
From the MHE measurements of each of the actual samples, an MHE plot is generated.
A typical example yields an MHE curve as shown in
Figure 43 .
Figure 43. MHE Plot of Analyte form an Analytical Sample