Thermo Scientific thermoscientific Vanquish Pumps C, Operating Manual

Page: 160 / 296

7 • Maintenance and Service
Page 160 Pumps C, Pumps F ( VC-Pxx, VF-Pxx)
Operating Manual
3. Remove some liquid from the tubing, for example, by shaking the
tubing.
4. Set up the system in such a way that the backpressure indicated in
the table is produced.
For example, install an appropriate backpressure capillary on the
pump outlet (outlet of the static mixer).
Pump Type Backpressure
VC-pumps 60 MPa (approximately)
VF-pumps 80 MPa (approximately)
5. Apply a flow rate of 2 mL/min using isopropanol.
6. To evaluate possible leakage, observe the liquid level in the tubing.
Finish the observation before a new seal wash cycle starts (after one
hour).
7. Depends on your observation:
Observation Remedial Action
Liquid level in the tubing remains
unchanged The piston seals seal tightly. Reconnect the
seal wash tubing to the seal wash detector
(port labeled
in ). This completes the test.
Liquid level in the tubing is
decreasing The seal wash seals are leaky:
1. Replace the seal wash seals.
2. Reconnect the seal wash tubing to the seal
wash detector (port labeled
in ).
3. Repeat the test starting with step 1.
Liquid level in the tubing is
increasing One or more piston seals are leaky.
Isocratic pump and quaternary pump:
1. Replace the piston seals in the pump head.
2. Reconnect the seal wash tubing to the seal
wash detector (port labeled
in ).
3. Repeat the test starting with step 1.
Binary pump and dual pump:
Continue with the next steps.
8. On the right pump head, remove the seal wash tubing that connects
the left pump head to the right pump head.
9. Depends on the pump type:
Pump Type Action
Binary pump Apply a flow rate of 2 mL/min for 100%
solvent A using isopropanol.
Dual pump Apply a flow rate of 2 mL/min for the left
pump using isopropanol.