Thermo Scientific thermoscientific Vanquish Pumps C, Operating Manual

Page: 106 / 296

6 • Operation
Page 106 Pumps C, Pumps F ( VC-Pxx, VF-Pxx)
Operating Manual
Items required
• Solvents suitable for purging
To flush out the isopropanol when operating the pump for the first
time, use solvents that are miscible with isopropanol. If they are not,
use an appropriate intermediate solvent.
• Silicone tubing
• Tissue
• Syringe
TIP For first time-operation: Silicone tubing and a syringe are available in
the ship kit.
Preparations
1. To be able to generate negative pressure, connect an autosampler
or another type of flow restriction to the pump outlet. If necessary,
close the pump outlet with a fitting plug.
2. On the purge/sensor unit, attach silicone tubing to the purge outlet
(if it is not yet connected).
3. Insert the syringe tip into the open end of the tubing.
4. Open the purge valve by turning the valve knob counterclockwise.
Follow these steps
1. In the Chromeleon software, set the channel that you want to purge
to 100%.
2. Optional: In the Chromeleon software, adapt the settings for the
purge flow or purge time if required.
3. Binary pump and dual pump: For purging from the keypad, verify
that you have selected the pump head (pump) for which you want to
perform the procedure.
4. Start the purge cycle. You can start the purge cycle from the
Chromeleon software or by pressing the PURGE button on the
keypad.
5. Use the syringe to draw enough solvent for filling the solvent line to
the purge outlet.
6. When the liquid enters the silicone tubing, examine the solvent line.
If the solvent line is completely filled with liquid and no air bubbles
are visible, stop the purge cycle (from the Chromeleon software or
by pressing the PURGE button).