Qiagen QIAsymphony SP, Operating Manual

Page: 70 / 216

QIAsymphony SP/AS Consolidated Operating Guide 12/2017 70
8.4.2 Reagent cartridges
The required reagent cartridges are determined by the QIAsymphony SP from the protocols that
were chosen by the user.
The reagent cartridges can be either from the same kit or from different kits.

Reagents required for the purification procedure are provided in prefilled, sealed reagent
cartridges.

Up to 2 reagent cartridges can be loaded into the “Reagents and Consumables” drawer.

For increased ease of use, reagent cartridges fit only in the correct orientation.
1. The user first vortexes the magnetic-particle trough and then removes the seal from the
magnetic-particle trough.
2. Remove the lids from the tubes and place into the appropriate slot to prevent mix-up.
3. When the piercing lid has been properly installed before loading, the reagent cartridge
is then automatically opened by the QIAsymphony SP, which eliminates manual
handling and pouring of reagents.

Each individual reagent in the reagent cartridge is labeled with a 2D bar code, enabling
tracking of reagents through the entire purification procedure.

Before the run starts, the system checks whether the reagent volumes are sufficient for the
chosen protocol.
Reagent cartridge holder
Magnetic-particle holder
Reagent troughs
Enzyme rack
Piercing lid
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