Qiagen QIAsymphony SP, Operating Manual

Page: 164 / 216

QIAsymphony SP/AS Consolidated Operating Guide 12/2017 164
Error code Description Comments and suggestions
76611 You selected X samples for
processing in this batch. However,
the calculation of the required
reagents and consumables was
based on Y samples. The new sample
count might require additional
reagents or consumables which must
be loaded before starting the run. To
avoid this, press
No and reduce the
number of selected samples. Do you
want do continue anyway?
More samples were assigned to a protocol than were defined
in the
Wizard/ACS and Number of Samples screen.
Perform one of the following:

Press No and unassign some samples. Try again.
 Press Yes and complete the Wizard. Then press R+C and
check whether there are any missing reagents or
consumables.
100011 Reference point X RP Y was not
recognized. Restart the run. If this does not resolve the error, restart the
QIAsymphony SP/AS instruments.
If the problem persists, contact QIAGEN Technical Services.
130725 Removing tips into waste failed: X. A Z-drive movement error occurred while discarding tips.
Check the tip chute and the tip disposal bag.
130906 The batch timer W has been expired
before it was evaluated. Nominal
time span: X, actual time span: Y,
timer overrun sec: Z. The time required for a protocol step was exceeded.
Note : Do not pause the run for an extended period of time.
Inventory scans (e.g., inventory scan of the “Eluate” drawer)
may result in the lysis time being exceeded. All samples in the
corresponding batch will be flagged as “unclear”.
140036 Not enough liquid available. Sample
volume is too small.
Or
Not enough liquid available Slot x
PosY Reagent Z.
This error code can occur during operation of the
QIAsymphony SP and during operation of the QIAsymphony
AS.
Samples may be flagged as “unclear” or “invalid”.
For the QIAsymphony SP :
Be sure to insert and to define the sample tube. Use only
compatible sample tubes or plates. For more information
about compatible sample tubes and plates, see
www.qiagen.com/goto/QIAsymphony.
Make sure that sample tubes and plates fit correctly in the
tube/plate carrier. Use an appropriately sized vessel for the
sample volume.
Note : Be sure to use at least the minimum sample volume
required for the protocol. Refer to the handbook of the
QIAsymphony Kit you are using for more information about
sample amounts and volumes.
For the QIAsymphony AS :
Ensure that the correct tube type is used. Ensure that there are
no bubbles on the surface of the liquid. If necessary centrifuge
the tube to remove any bubbles. Ensure that the required
amount of liquid is available and that the tube is positioned
correctly. If necessary, add more liquid.
140055 Wrong number or position of tube
cartridges detected in the extractor. The number of sample prep cartridges in the inventory differs
from the number detected by the sensors on the magnetic
head. This could be due to incorrect placement of sample prep
cartridges, or sample prep cartridges could have been lost
during transfer.
Check the worktable, execute clean up, and restart the batch.
140056 Wrong number of rodcover detected
on rodcover plate in the extractor.
The number of 8-Rod Covers detected in the inventory differs
from those detected by the sensors on the magnetic head.
Check the worktable, perform worktable cleanup and restart
the batch.