Qiagen QIAsymphony SP, Operating Manual

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QIAsymphony SP/AS Consolidated Operating Guide 12/2017 104
16. While the integrated run is being processed on the QIAsymphony SP, load the QIAsymphony
AS. Open the “Eluate and Reagents” and “Assays” drawers.
17. Load assay rack(s) into the appropriate precooled adapter(s) and place them onto the “Assay”
slot(s).
18. Press the orange Assay Rack button, and press Load in the next screen to load the assay
racks(s) virtually.
19. Fill each reagent tube with the required volume of appropriate reagent and place the reagent
tubes, without lids, into the appropriate positions of precooled adapters for reagents.
20. Press the orange Reagent Rack button, and press Load in the next screen to load the reagent
rack(s) virtually.
21. Place the prepared assay adapter(s) onto the appropriate slot(s).
Important
Ensure that reagents are completely thawed. If desired, enter a kit bar code for
each assay.
22. Load disposable filter-tips into the “Eluate and Reagents” and “Assays” drawers. Load at least
the required number of each tip type.
23. Close the “Eluate and Reagents” and “Assays” drawers and start the inventory scan.
24. Assay setup will start automatically after the inventory scan was performed successfully and
the sample preparation for the integrated batch was finished.
25. When running more than one integrated batch, remove the previously finished integrated
batch in the
Integrated Setup overview. Reload the QIAsymphony AS “Eluate and Reagents”
and “Assays” drawers to continue the next AS batch.
12.3.1 Defining an integrated run
When defining an integrated run, screens guiding you through the steps appear on the
touchscreen.
It is only possible to define an integrated run if an eluate rack and a transfer frame have been
loaded on “Eluate slot 1” of the QIAsymphony SP. To save time, the system checks for the transfer
frame during the rack carrier inventory scan.