Qiagen QIAsymphony SP, Operating Manual

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QIAsymphony SP/AS Consolidated Operating Guide 12/2017 48
7 QIAsymphony SP Features
The section describes how to operate the QIAsymphony SP instrument, including how to load and
unload the worktable.
The QIAsymphony SP performs fully automated purification of nucleic acids using magnetic-
particle technology. Samples can be processed in batches of up to 24 samples. The instrument
controls integrated components including a lysis station, 4-channel pipetting system, robotic
gripper, and an array of magnetic rods that are protected by rod covers. These rods can pick up
or release magnetic particles in the wells of a sample prep cartridge, depending on whether the
magnetic rods are inserted in the rod covers or not.
The QIAsymphony SP is preinstalled with various protocols and corresponding Assay Control Sets
for purification of RNA, genomic DNA, and viral and bacterial nucleic acids. The user loads
reagents (in prefilled, sealed reagent cartridges) and consumables into the appropriate drawer,
loads the samples, and selects a protocol using the touchscreen. The user then starts the protocol,
which provides all necessary commands for sample lysis and purification. A fully automated
inventory scan (either after closing the individual drawers or before the run starts) helps to ensure
that the QIAsymphony SP is correctly set up for the protocol.
7.1 Workflow principle
Sample input Waste compartment Magnetic head
Reagent cartridges “Elution slot 1” (cooled) Robotic arm
Filter-tips “Elution slots 2–4” Tip waste chute
Consumables Lysis station (heated) Tip park station
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