QIAsymphony SP/AS Consolidated Operating Guide 12/2017
169
Cycler file errors
Error
Comments and suggestions
Cycler file is not created
or is not correct for the
cycler.
The QIAsymphony SP/AS instruments automatically create a cycler file when an assay run is
finished. The format of the cycler file depends on the assay rack type.
Ensure that the correct cycler file format for the assay rack(s) is defined in the Assay Parameter
Set. If necessary, modify the cycler file format in the Assay Parameter Set using the
Process
Definition
editor tool of the QIAsymphony Management Console.
If the required assay rack format for a particular cycler file format is not available to be selected
in the QIAsymphony Management Console, ensure that the available assay racks are updated
in the QIAsymphony Management Console. See the
QIAsymphony Management Console User
Manual
for more details about how to do this.
If the problem persists, contact QIAGEN Technical Services.
Result file AS errors
Error
Comments and suggestions
The final result file is not
created. / Only a
preliminary result file is
visible.
The QIAsymphony SP/AS instruments create a preliminary result file when an assay run is
started. The final result file is created when
Remove
is pressed at the end of an assay run.
If using automatic transfer, check in the related folder to see if the correct printer is listed.
Ensure that the assay run has been removed before attempting to download the result file.
Ensure that you are looking in the correct folder for the QIAsymphony SP result files or the
QIAsymphony AS result files. The correct folder is
log/Results/SP
or
log/Results/AS
.
Sample status.
If errors/problems occur during an assay run, sample status can be affected.
If samples were successfully processed, the sample status is “valid”. If the batch was paused, the
samples will be “unclear” and if, for example, cooling problems occur during a run, the sample
status may be “unclear”. If problems occur during master mix or sample transfer, the sample
status is “invalid”.
If a QIAsymphony SP rack file is used on the QIAsymphony AS, the sample status will only be
changed if errors/problems occur during the assay run. If sample status is changed, the reason
for this change will be recorded in the QIAsymphony AS result file. The message, the message
ID, and the sample status is listed in the
Detailed Run Information
section of the QIAsymphony
AS result file.
Loading information file errors
Error
Comments and suggestions
The loading information
file is not created.
A loading information file should be generated after pressing
Queue
.
Ensure that you are looking for the loading information file in the correct folder. The correct
folder is
\log\LoadingInformation
.
If using the automatic file transfer tool of the QIAsymphony Management Console, check in the
related configuration to see if the correct printer is listed.
Log file errors
Error
Comments and suggestions
General transfer
problems.
Ensure that the QIAsymphony SP/AS instruments are connected to the network when using the
QIAsymphony Management Console for file transfer.
Ensure that the USB stick is correctly plugged in and that the small LED on the USB stick is
glowing.
Summary of Contents for QIAsymphony SP
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