Qiagen QIAsymphony SP, Operating Manual

Page: 84 / 216

QIAsymphony SP/AS Consolidated Operating Guide 12/2017 84
2D bar codes.

If the reagent cartridge is sealed and not pierced, the liquid level of all reagents in the reagent
cartridge is set to the original value. An additional liquid-level check will not be performed.

Both reagent cartridge slots are scanned.
Important
Do not mix enzyme racks, buffer, or magnetic-particle troughs from different
reagent cartridges.
Laser scan — tip rack slots

All 18 tip rack slots are scanned to determine the type of tip rack loaded.

All tip rack slots in which a tip rack was detected are scanned to determine the number of
tips. If a tip is detected in the first and last position of the tip rack, the tip rack will be
categorized as full. If the first or last tip is missing, a full scan will be performed to determine
the number of tips in the tip rack.
Laser scan — unit boxes

The unit box slots are scanned to detect the presence of unit boxes in the 4 slots.

Afterwards, the type (8-Rod Cover or sample prep cartridge) and number of consumables are
determined.
Liquid-level scan of detected reagents
This scan is only performed if the liquid level is not known (e.g., for a partially used reagent
cartridge).

Liquid-level scan of detected reagents.

Liquid-level check of the buffer bottle (if detected).

Liquid-level check of the Accessory Trough (if detected).