Promega Maxprep AS9100 Operating Manual Download Page 1

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14831T

Maxprep™ Liquid Handler

Operating Manual

Instructions for use of AS9100, AS9101, AS9200 and AS9201

TM509

Revised 1/19

Summary of Contents for Maxprep AS9100

Page 1: ...Maxprep Liquid Handler Operating Manual Instructions for use of AS9100 AS9101 AS9200 and AS9201 TM509 Revised 1 19...

Page 2: ......

Page 3: ...Components 9 1 7 Precautions 10 1 8 Safety Symbols and Marking 11 1 9 Environmental Requirements Operating Shipping and Storage Conditions 13 2 Hardware Overview 15 2 1 Hardware Features 16 2 2 UV Li...

Page 4: ...53 5 1 Maintenance 53 5 2 Methods 55 5 3 Starting a Method 56 5 4 Error Recovery 64 5 5 Reports 67 5 6 Accessing Reports 70 6 Cleaning the Maxprep Liquid Handler 73 6 1 Weekly Cleaning 73 6 2 Dealing...

Page 5: ...on 87 11 Appendix Configuring the PC 89 11 1 Maxprep Liquid Handler and PC 89 11 2 Glossary 90 11 3 Adjusting Volume Settings 92 11 4 Configuring Date and Time Settings 92 11 5 Managing Local Windows...

Page 6: ...4 Maxprep Liquid Handler TM509 Revised 1 19 Table of Contents...

Page 7: ...d run specific information can also be tracked in the Promega Portal software to facilitate information transfer between the Maxprep Liquid Handler and the Maxwell RSC and Maxwell RSC 48 Instruments R...

Page 8: ...SC nucleic acid purification kits or with samples other than those defined within the product limitations of the specific Maxwell RSC kit being used Post processing methods provided with the Maxprep L...

Page 9: ...umidity 20 to 70 C 10 to 90 non condensing UV Bulb optional Average lifetime approximately 10 000 hours length 9 2in 233mm base face to face diameter 15mm 11W 0 43A current 25 6V UV C UV output 32W at...

Page 10: ...ion Shaking 3mm Orbit Up to 2500rpm Temperature range ambient to 105 C Pipetting Specifications for Channels Disposable Tips Size Volume Accuracy Precision CV 50 l 1 l 5 0 5 0 5 l 2 5 2 0 50 l 2 0 1 0...

Page 11: ...0mm Sample Tube Carriers 3 12 13mm Sample Tube Carriers 3 15 17mm Sample Tube Carriers 1 Box of Maxprep 50 l Conductive Disposable Tips Filtered 5 96 1 Box of Maxprep 300 l Conductive Disposable Tips...

Page 12: ...on tubes Use only Promega supplied consumables tips troughs preprocessing plates Do not reuse cartridges plungers or elution tubes If the equipment is used in a manner other than that specified by Pro...

Page 13: ...ety Instructions Save these instructions Safety Symbols and Markings 5295MA eps Warning Risk of personal injury to the operator or a safety hazard to the instrument or surrounding area Warning Hot sur...

Page 14: ...85 USA 249469 Serial Number Manufacturer 10928MA 40 VAC A 2 5A 20mm S4000 3456789 Made in USA 050 9800 Rev D Maxwell CSC Promega BioSystems Sunnyvale Inc 645 N Mary Ave Sunnyvale CA 94085 USA 249469 C...

Page 15: ...porting 350lb Avoid dusty areas Choose a location that has good air circulation and is not exposed to direct sunlight Avoid electrically noisy power sources e g power generators Do not install in a lo...

Page 16: ...14 Maxprep Liquid Handler TM509 Revised 1 19 Introduction Notes...

Page 17: ...e instrument has a large door for access to the inside of the system To open the door grip the handle pull toward you and then slide the door upwards To close the door grip the handle pull toward you...

Page 18: ...hin the instrument Included on the front arm of the gantry is a 1D 2D bar code reader that can automate the process of reading tube bar codes that are loaded on the system in tube carriers Figure 2 Ga...

Page 19: ...ddle are facing toward the center of the tool rack 2 2 UV Light optional Maxprep Liquid Handler Cat AS9101 and AS9201 are provided with a UV light system UV lights are present on the top left and top...

Page 20: ...with the system and their track positions and functions Carrier Type Number Provided Track Positions Function Utility Carrier 1 1 6 Static carrier cannot be removed from system with heater shaker and...

Page 21: ...ple Tubes 3 Maxprep Carrier 10mm Sample Tubes 3 22 23 24 Removable tube racks can hold primary sample tubes and elution storage tubes Provided with the system are three types of tube carriers that can...

Page 22: ...waste position with a black removable tip eject bar at the back and a calibration block at the front Figure 5 Remove the tip eject bar from the system for cleaning and as instructed during maintenance...

Page 23: ...emoving the waste bin lift it out toward the right while angling up the right side of the bin to avoid hitting the tip eject bar Return the waste bin to the system by angling it under the tip eject ba...

Page 24: ...22 Maxprep Liquid Handler TM509 Revised 1 19 Hardware Overview Notes...

Page 25: ...ncerns or limitations with connecting the Maxprep Liquid Handler PC and any Maxwell Tablet PCs to your network Recommended Domain policy settings when the Maxprep Liquid Handler PC is connected to you...

Page 26: ...ation Software by double clicking the icon on the PC desktop Figure 6 On Off Switch 3 3 Shutting Down the Maxprep Liquid Handler 1 Shut down the Maxprep Software by pressing the X in the upper right c...

Page 27: ...see Section 5 3 Starting a Method Results The Results button opens the Results Screen where it is possible to review print and export any of the run reports from previous chemistry and maintenance pro...

Page 28: ...et administrator specific instrument settings with Administrator only available to users with Administrator level access to the Maxprep software see Section 4 2 Software Settings and Section 4 3 Admin...

Page 29: ...icon with a red circle containing an exclamation point indicates that the connection to Portal software has been lost Results from the last run will be exported to Portal software when the connection...

Page 30: ...e appearance of the Settings screen Figure 8 is tailored to the Maxprep software access level assigned to the Windows user account see Appendix A Windows user account with user level access to the Max...

Page 31: ...xprep software version installed on the PC Maxprep Software Revision the Maxprep software revision number of the software version installed on the PC Instrument Software Version the instrument control...

Page 32: ...logs to the specified path or press Cancel to return to the Settings screen without exporting logs 4 After pressing OK the Export screen is displayed indicating that the log files were successfully e...

Page 33: ...en allow the administrator to manage Methods installed in the software import or delete Labware Manage Kits installed in the software configure Preferences for the Maxprep software define an Instrumen...

Page 34: ...reen The Administrator button will only be visible if the currently logged in operator has administrator level rights within the Maxprep software Figure 13 Maxprep Administrator screen The Administrat...

Page 35: ...For entries with a folder icon select the entry to view variant methods Use the filter check boxes on the left side of the screen to display only certain types of methods You can filter by Method Type...

Page 36: ...th the method Version Number The version number of the method Icon for creation of a new variant method based on the settings for an existing method Icon for editing an existing variant method Icon fo...

Page 37: ...when selected on the right side of the screen 6 Select the blue rectangle s corresponding to the desired package file s to highlight and then press the OK button to import the selected methods Note W...

Page 38: ...A The new variant method will initially be populated with the values from the original method or variant method Changes made to the method will be saved as a new variant method To edit an existing va...

Page 39: ...s are within the absolute range set by Promega iii The default value is within the specified range If there is an issue with any of these conditions the software will automatically reset the offending...

Page 40: ...formation present Mark this method as a favorite so it is always sorted to the top of the variant methods list Name The name of the method Description A description of the method Icon for creation of...

Page 41: ...lled in the Maxprep software on the Labware screen Figure 16 by touching the Labware button from the Administrator screen Figure 13 Figure 18 Labware screen This screen displays a list of the labware...

Page 42: ...ep software 1 Save the labware labware file on the PC attached to the Maxprep Liquid Handler 2 If not already running start the Maxprep software by double clicking the software icon on the desktop 3 N...

Page 43: ...ng labware will be deleted The warning will ask whether you wish to proceed with import press Yes to delete the existing labware or No to cancel the import 7 A Labware Import selection screen is displ...

Page 44: ...re To delete a labware from the Maxprep software press the X on the right side of the labware list entry A Warning screen will be shown asking whether the labware should be permanently deleted Select...

Page 45: ...un report Export to PDF Checking this box will create a PDF version of exported results in addition to the tab delimited text file version of the results Export to Excel Checking this box will create...

Page 46: ...es screen From the Common Settings tab the administrator can allow use of expired kits require entry of Maxwell kit lot information allow deletion of results specify whether reports from aborted runs...

Page 47: ...eck this box to have the PC generate a sound when a method run is completed Play sound on error Check this box to have the PC generate a sound if an error occurs during a method run on the instrument...

Page 48: ...SL Encrypted connection On the right side of the screen the administrator can specify under what conditions and to whom e mail notifications will be sent Options include Send mail when method is compl...

Page 49: ...references screen From the E mail Settings tab the administrator can specify e mail server settings under what conditions e mail notifications should be sent and the distribution list to which e mails...

Page 50: ...her method specific data between multiple modules check the Enable Portal Sample Tracking check box on this tab By default the Portal software is installed on the PC connected to the Maxprep Liquid Ha...

Page 51: ...problems with Portal that are caused by issues with the data Data that has been cleared from export will not be sent to the Portal software and will only exist in local reports A prompt to save or di...

Page 52: ...s week this month the past 6 months this year or all functions performed over the life of the instrument Press the column headers to sort the records based on column contents Press the desired row to...

Page 53: ...or Cancel you will be returned to the Administrator screen Figure 26 Audit Records screen A user readable record of events that have occurred in the Maxprep software can be viewed by the administrator...

Page 54: ...52 Maxprep Liquid Handler TM509 Revised 1 19 Maxprep Software Notes...

Page 55: ...to access the Maintenance screen Figure 29 Listed on the Maintenance screen are methods designed to ensure your instrument is in working order The UV Sanitization method is also included depending on...

Page 56: ...conductive liquid level sensing Because the Weekly Maintenance method includes the functions tested in the Daily Maintenance method it is not necessary to run both Daily Maintenance and Weekly Mainten...

Page 57: ...ty of Maxwell RSC purification kits and to perform a variety of post processing processes e g quantitation PCR setup qPCR setup normalization etc Please refer to your specific Maxwell RSC reagent kit...

Page 58: ...id Handler 5 3 Starting a Method From the Home screen Figure 30 select the Start button to begin the process of running a method Figure 30 Maxprep software Home screen Selecting the Start button begin...

Page 59: ...iversal total nucleic acid samples Catalog ID The catalog number for the kit if any associated with this method Version The version number of this method Use the filter check boxes on the left side of...

Page 60: ...t example AS13303221872020 05 where the product catalog number is AS1330 the kit lot number is 322187 and the expiration date is 2020 05 Only if the bar code entered has this format will a method be s...

Page 61: ...shown in the title bar of the Method Run screen Summary information for the method is displayed on the left side of the screen To start the run close the door of the Maxprep Liquid Handler and press...

Page 62: ...the deck of the Maxprep Liquid Handler More detailed information regarding the specifics of the variables that need to be entered and the deck setup instructions for preprocessing quantitation amplifi...

Page 63: ...door before pressing the Next button While the method runs the method run screen will update with information regarding the current step in the method and display a progress bar indicating progress th...

Page 64: ...f you need to abort the method run currently in progress press the Abort button If there is a need to perform an immediate stop of the Maxprep Liquid Handler during a run press the power button to pow...

Page 65: ...ce of unexpected labware on the deck of the instrument Always use care when setting up the instrument and confirm that the deck configuration specified by the method setup instructions matches the phy...

Page 66: ...mega com to schedule a service visit prior to running the system again 5 If there are any tips or tools loaded on the pipetting channels you will need to remove these manually a With the Maxprep Liqui...

Page 67: ...Liquid Handler TM509 Revised 1 19 Operating the Maxprep Liquid Handler Figure 37 Squeeze Drive Turn the squeeze drive in the direction indicated to release any tools or pipette tips from the pipettin...

Page 68: ...rument 7 Press the power button to turn on the Maxprep Liquid Handler Restart the computer Start the Maxprep software by double clicking the Maxprep software icon on the desktop 8 From the Home screen...

Page 69: ...p Liquid Handler details software version firmware version instrument name serial number etc A second tab on the report displays the method specific variable settings that were used for this method Fi...

Page 70: ...to navigate to the folder in which the report files should be saved Press the Export button to navigate to the file location where the report should be exported and then press the OK button The Maxpre...

Page 71: ...ep Liquid Handler You can use the tab delimited format file txt with Laboratory Information Management Systems i e LIMS An example of the tab delimited format file is shown in Figure 41 Figure 41 Exam...

Page 72: ...see a detailed view of the report data from that method run Figure 43 Administrators can delete records from the software by pressing the X icon to the right of the desired report row if the option t...

Page 73: ...09 Revised 1 19 Operating the Maxprep Liquid Handler Figure 43 Method report An example of the sample tracking information present in a method run report The second tab on the report displays variable...

Page 74: ...72 Maxprep Liquid Handler TM509 Revised 1 19 Operating the Maxprep Liquid Handler Notes...

Page 75: ...e the method has started open the door of the instrument to clean the instrument While cleaning the instrument during the Weekly Maintenance method it is possible to manually move the gantry of the sy...

Page 76: ...backstop of the instrument Figure 44 Tip eject bar and plastic waste bin To the right of the carriers is a waste position that has a removable black tip eject bar as well as a calibration block On the...

Page 77: ...es Wipe well once visible material is removed In case of spills in the instrument where there is a potential biohazard wipe up the spill with paper towels and wash the spill area with a detergent solu...

Page 78: ...76 Maxprep Liquid Handler TM509 Revised 1 19 Cleaning the Maxprep Liquid Handler Notes...

Page 79: ...ss to the Maxprep software have the ability to change certain functions If you do not have administrator level access contact your site administrator Power failure during a run In the event of a power...

Page 80: ...ns are observed when starting a method a full restart of the system is required Shut down the Maxprep software and turn off the Maxprep Instrument Restart the Maxprep Instrument and restart the Maxpre...

Page 81: ...e When using a USB flash drive insert it before running any method and do not remove it until the method is finished Do not insert or remove a USB flash drive while instrument is running Since USB fla...

Page 82: ...80 Maxprep Liquid Handler TM509 Revised 1 19 Troubleshooting Notes...

Page 83: ...e contact Promega or your local Promega representative for support Visit the Promega Web site at www promega com for the contact information for the Promega branch or distributor nearest you If furthe...

Page 84: ...82 Maxprep Liquid Handler TM509 Revised 1 19 Instrument Service Notes...

Page 85: ...Premier Warranty Upgrade includes all parts labor and on site service visits by a factory trained service technician within 2 business days where available Additionally it includes one annual on site...

Page 86: ...e Support Yes within 5 business days Yes within 2 business days Preventive Maintenance No available separately Yes 1 Application Support Yes limit 2 visits Yes limit 3 visits Maxprep Liquid Handler In...

Page 87: ...d repair of your instrument This Warranty may be renewed in one year terms provided the renewal is requested prior to the expiration of the current Warranty or Service Agreement Promega agrees as its...

Page 88: ...w UV light 1 each AS9101 Maxprep Liquid Handler RSC 48 Carriers 1 each AS9200 Maxprep Liquid Handler RSC 48 Carriers w UV light 1 each AS9201 1 1ml Square Well V Bottom Deep Well Plate 25 pack V6821 N...

Page 89: ...ed and can be handled without exposing personnel to health hazards Circle the type of material used in the instrument Chemical Biological Radioactive Briefly describe the decontamination procedure per...

Page 90: ...88 Maxprep Liquid Handler TM509 Revised 1 19 Certificate of Decontamination Notes...

Page 91: ...n antivirus program please ensure it is set to manual not automatic update Antivirus scanning will slow the performance of the PC and may affect the performance of the Maxprep Liquid Handler while it...

Page 92: ...ows 10 Operating system this section describes terms and actions common to this operating system Lock Screen Windows user accounts with password protection or network domain user accounts need to sign...

Page 93: ...h buttons on the left side of the Taskbar Swipe upward from the bottom of the screen to expose the Task bar Start Menu The Start menu Figure 46 is the major access point for settings and functions on...

Page 94: ...nts in the Windows operating system to one of two Promega access level groups The sections below detail the process of adding and removing local Windows user accounts that can be assigned to the acces...

Page 95: ...internet domain your IT department should be consulted on how to add users to the domain that can be used on the PC Do not use these instructions to create a new local account on the PC if accounts o...

Page 96: ...PC 11 5 Managing Local Windows User Accounts continued 3 Click the Accounts button on the Windows Settings screen to open the Accounts screen Figure 48 Figure 48 Accounts screen Settings related to W...

Page 97: ...new user account click the sign next to the Add someone else to this PC heading This will open the Create an account for this PC screen Figure 50 Figure 50 Creating a new account Enter name and passw...

Page 98: ...e button to open the Change account type screen Figure 52 Using the drop down menu under the Account type heading choose whether this user should have Administrator or Standard User access rights with...

Page 99: ...ed into the PC will not have access to run the Maxprep Liquid Handler software unless they have been added to one of the Promega access groups Section 11 6 Do not use these instructions to remove an a...

Page 100: ...t Once you have changed to the account to be removed from the Desktop screen click the Start button in the lower left corner of the screen Choose the Accounts icon from the left side of the Start menu...

Page 101: ...o configure options and settings for the Tablet PC 6 Click the Accounts button on the Windows Settings screen to open the Accounts screen Figure 55 Figure 55 Accounts screen Settings related to Window...

Page 102: ...l remove all of the user account data from the PC Click Delete account and data to remove the selected account or Cancel to retain the account and return to the Accounts screen Note Removing a Windows...

Page 103: ...own to see the Promega folder Click the Promega folder to expand the items within the folder 4 Right click the Maxprep item to open the options popup Click the Pin to Start item Figure 57 Panel A This...

Page 104: ...nistrator access rights and administrator level access rights to the Maxprep software Note The PromegaServiceTechnicians group is only for use by Promega Service Representatives Do not assign users to...

Page 105: ...essing Users and Groups Panel A Touch the Search button in the taskbar and enter lusrmgr msc into the search window to bring up the lusrmgr msc item Panel B Selecting the lusrmgr msc item from the sea...

Page 106: ...e Members section of the window click the Add button 8 Now you will see the Select Users screen Figure 60 Panel B In the section of the screen titled Enter the object names to select type the username...

Page 107: ...er button from the Start menu opens the Power options popup This popup presents options for managing the PC power state 13 To check the appropriate access level for the account sign in to the PC as th...

Page 108: ...Promega Access Level Group Access levels within the Maxprep Liquid Handler software are controlled by assigning a Windows user account to a Promega access level group The instructions below detail th...

Page 109: ...r msc should appear click this item to open the Local Users and Groups Local screen Figure 63 Panel B Figure 63 Accessing Users and Groups Panel A Click the Search button in the task bar and enter lus...

Page 110: ...on of the screen click the name of the user you wish to remove from this Promega access group Figure 65 Figure 65 Removing a Windows user account from a Promega access level group Click the Windows us...

Page 111: ...er TM509 Revised 1 19 Appendix Configuring the PC Figure 66 Power options popup Clicking the Power button from the Start menu opens the Power options popup This popup presents options for managing the...

Page 112: ...ck the Accounts icon to open the Account information popup Figure 67 Figure 67 Account information popup Accessed from the Start menu the Account information popup displays the Windows user accounts o...

Page 113: ...top screen swipe upward from the bottom of the screen to expose the Task bar and then touch the Start button on the left side of the Task bar 3 Click the Power icon to open the Power menu Figure 68 Fi...

Page 114: ...program please ensure it is set to manual not automatic update Antivirus scanning will slow the performance of the PC and may affect the performance of the Maxprep Liquid Handler while it is processi...

Page 115: ...t your site IT department when connecting to a network printer 1 Close the Maxprep software if it is open Click the X in the upper left corner of the screen and then confirm that you want to exit 2 Fr...

Page 116: ...must be connected to a network to access network printers 5 Click the button next to the Add a printer or scanner option The PC will automatically search the network for available printers that can b...

Page 117: ...xwell is a registered trademark of Promega Corporation Maxprep is a trademark of Promega Corporation Excel and Windows are registered trademarks of Microsoft Corporation LpH is a registered trademark...

Page 118: ...N 2800 WOODS HOLLOW ROAD MADISON WI 53711 5399 USA TELEPHONE 608 274 4330 www promega com 2017 PROMEGA CORPORATION ALL RIGHTS RESERVED PRICES AND SPECIFICATIONS SUBJECT TO CHANGE WITHOUT PRIOR NOTICE...

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