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PerkinElmer 200 UV/VIS Series, User Manual

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PerkinElmer 200 UV/VIS Series User Manual Download Page 81

This chapter describes how to create Methods on your Series 200 UV/VIS LC Detector. It 
includes the following information:

•

Understanding Methods

•

Viewing the Method Screen

•

Editing the Current Method

•

Creating a New Method

•

Storing a Method

•

Recalling a Method from the Directory

•

Editing a Stored Method

•

Running a Method

Using Method Mode

4

Summary of Contents for 200 UV/VIS Series

Page 1: ...Series 200 UV VIS Detector User s Manual...

Page 2: ...tial damages in connection with furnishing performance or use of this material NOTE TotalChrom is the updated version of the software previously marketed as Turbochrom This product is compatible with...

Page 3: ...vely as industrial commercial or medical test equipment It is exempt from the technical standards specified in Part 15 of the FCC Rules and Regulations based on Section 15 103 c Europe All information...

Page 4: ...and should be read before proceeding to the installation section Symbols and Conventions Used in this Manual The following graphic symbols and graphic text formats are used in this manual to set apart...

Page 5: ...that could cause per sonal injury if precautions are not followed This can be any type of hazard When you see this symbol refer to the safety pages in the manual for further information Consulter les...

Page 6: ...ing conductor disconnected D brancher le cordon d alimentation avant d ouvrir da continuit des masses est essentielle Pour un fonctionnement sans danger Ne jamais uti liser l quipment si le fil de ter...

Page 7: ...e explains this procedure The following illustration contains safety information for the procedure Figure S 3 Front Panel with door open for a lamp replacement Turn the Detector power switch to the OF...

Page 8: ...outside the instrument or disconnection of the protective ground terminal may impair the protection provided by the instrument Do not operate the instrument with any covers or parts removed Do not at...

Page 9: ...ircuit the fuse holder The instrument is to be positioned in a clean area free of dust smoke vibration and cor rosive fumes out of direct sunlight and away from heating or cooling units or ducts The i...

Page 10: ...s The Series 200 UV VIS Detector was designed for indoor use and will function correctly under the following ambient conditions Temperature 10 35 C Relative Humidity 20 80 noncondensing Altitude 0 200...

Page 11: ...an cause eye injury wear suitable protective goggles when changing or adjusting the lamp If the detector has been operated recently the lamp assembly will be very hot Check carefully before touching t...

Page 12: ...low 10 to 12 minutes for the monochromator to become completely filled Continue this nitrogen flow while the instrument is in use Purging is particularly important when the instrument is removed from...

Page 13: ...ent is able to withstand transient overvoltage according to Installation Cate gory II as defined in IEC 1010 1 Pollution Degree This equipment will operate safely in environments that contain noncondu...

Page 14: ...ory Cell phones beepers and other similar devices operate in a much higher frequency range and do not cause interference Fuses The following fuses are in the power entry module to protect the instrume...

Page 15: ...he Series 200 UV VIS Detector and its other system compo nents by a PerkinElmer Service Engineer to ensure its operation within published specifications These tests consist of measuring the most impor...

Page 16: ...the applicable Material Safety Data Sheets MSDS or OSHA ACGIH or COSHH documents Venting for fumes and disposal of waste must be in accordance with all national state and local health and safety regul...

Page 17: ...an optional solvent cabinet for example the SEC 4 Do not keep buffer solutions in the system when the instrument is not in use since salt may crystallize out Do not use acidic chlorides with pH less...

Page 18: ...f column s being used Know the relative polarity and miscibility of the solvents being used Solvents with Low Boiling Points Do not use liquids that have a boiling point less than 30 C Buffers Exercis...

Page 19: ...ile phase from the pump and flush solvent are made of titanium fluoropolymers or glass This makes the Series 200 UV VIS Detector inert to the most common mobile phases that are used for biological ass...

Page 20: ...re in Table S 3 Table S 3 Auto Ignition Temperatures of Common LC Solvents Do not use carbon disulfide or other solvents which have an auto ignition temperature below 110 C Solvent Auto Ignition Tempe...

Page 21: ...d attention If you are injecting clean samples and there is no other apparent cause for the pressure build up remove disassemble and clean the flowcell If you are injecting dirty samples the flowcell...

Page 22: ...VIS Detector weighs only 11 4 kg 25 lbs improper lifting can cause injury to the back Exercise caution when lifting High Voltage The Series 200 UV VIS Detector contains high voltage up to 375 volts B...

Page 23: ...Safety and Regulatory Information Warnings from the User s Manual S 21...

Page 24: ...Safety and Regulatory Information Warnings from the User s Manual S 22...

Page 25: ...Nitrogen Purge S 10 Detector Flowcell S 10 Storage Conditions S 11 Dimensions and Weight S 11 Additional Electrical Considerations and Warnings S 11 Quality Control Good Laboratory Practices S 13 Def...

Page 26: ...2 Unpacking Your Detector 2 4 Items Included 2 4 Making Electrical Connections 2 5 Checking Electrical Power Requirements 2 5 Set ting the Operating Voltage and Installing the Fuse 2 5 Installing the...

Page 27: ...ining a Sequence 5 4 Rules for Defining a Sequence 5 4 Identifying the Methods to Include in a Sequence 5 5 Procedure for Defining a Sequence 5 6 Procedure for Defining a Sequence continued 5 7 Settin...

Page 28: ...8 1 8 Theory of Operation 8 2 Beer s Law 8 2 Optical Design 8 4 Design Considerations 8 6 Application Notes 8 7 Wavelength Selection 8 7 Detector Response Time 8 8 Enabling External Control 9 1 9 Con...

Page 29: ...information A Description of the Series 200 UV VIS LC Detector The Series 200 UV VIS LC Detector s Features and Benefits The Major Components of the Series 200 UV VIS LC Detector Powering Up the Dete...

Page 30: ...function keys on a keyboard located on the front panel of the instrument A backlit Liquid Crystal Display LCD located directly above the keyboard shows both status information about the instrument an...

Page 31: ...NOTES TO REMEMBER WHEN USING THE DETECTOR When using the S200 Detector with Turbochrom the detector must display the top level method or manual screen before attempting to take control from Turbochrom...

Page 32: ...llowing components Keyboard and Display All instrument functions are accessed using the keyboard Status information is shown on the screens appearing in the display An overview describing how to perfo...

Page 33: ...in Components of the Series 200 UV VIS LC Detector Getting Started with the Detector 1 5 Figure 1 2 Front Right Side View of UV VIS Detector Input Output Connector Panel Keyboard and Display Access Do...

Page 34: ...el The rear panel contains the Power Input Module and the serial ports for the UV VIS Detector The power module accepts the AC line cord and contains the system fuses and volt age selector card You ca...

Page 35: ...s the power switch located on the rear panel of the Detector to the ON position The Copyright and Self Test Screen first appears on the Liquid Crystal Display The display is located directly above the...

Page 36: ...nnot save the parameters that you set in Manual Mode The set of parameters that appear the first time you start the detector are the default settings The next time you start the detector in Manual Mod...

Page 37: ...the information on the screens and also to start or stop certain operations on the UV VIS Detector As you move from one screen to another the screen defined function keys change The functions of the v...

Page 38: ...y is equivalent to the right cur sor arrow key and operates whenever the right cursor key is operative CE Press CE to clear a typed value and redisplay the original value Detector Control Keys reset P...

Page 39: ...rement the steps following it by one for example when adding methods in a sequence delete Press to delete the current step and decrement the steps following it by one Use the cursor arrow keys to acce...

Page 40: ...Started with the Detector Using the Keyboard and Display 1 12 Screen Hierarchy Diagram The following figure provides an overview of the hierarchy of the detector s screens Figure 1 5 Screen Hierarchy...

Page 41: ...s an overview of how to perform the following basic operations using the LC Detector keyboard Selecting screen options Selecting data fields and entering numeric information Entering non numeric infor...

Page 42: ...creen corresponding to the selected option appears Typical examples include selecting options from the top level Main Screen and the Configure Screen as shown here F1 F8 To F1 CONFIGURE SCREEN F2 F3 F...

Page 43: ...press the Enter key For example on the Method Edit Screen shown below the data field for the TIME is highlighted To accept the current entry and move to and highlight the Wavelength field press the ri...

Page 44: ...es a non numeric entry the options for that field appear as screen defined soft keys To change the current entry in the highlighted field press the corresponding function key F1 through F8 For example...

Page 45: ...down The caret indicates the selected line The following example shows the three most common types of screen lists the Directory Screen Sequence Screen and the Timed Events Screen caret DIRECTORY SCR...

Page 46: ...yboard and Display 1 18 Responding to Screen Prompts To respond to a Y N prompt appearing in the Message line of a screen such as the one shown in the following screen press the numeric key to select...

Page 47: ...ns When you finish making changes to a screen press the key to save those changes Changes you make to a method screen will remain in effect only until you turn off the power to the Detector To permane...

Page 48: ...glass envelope located in flowcell drip pan Display Graphic LCD module 240 X 64 pixel Series 200 standard Noise filter Digitally controlled active filter with response RSP or peak width PW selectable...

Page 49: ...ls that the unit is ready to run TE1 and TE2 programmable outputs Control inputs EXT RUN same as pressing the run front panel key EXT STOP same as pressing the reset front panel key AZ same as pressin...

Page 50: ...Getting Started with the Detector Instrument Specifications 1 22...

Page 51: ...bes how to install your Series 200 UV VIS LC Detector It includes the following information Preparing your laboratory Unpacking your detector Making the electrical connections Making plumbing connecti...

Page 52: ...onstant relative humidity between 20 and 80 without condensation Use care when working with hazardous solvents or solvents that produce hazardous by products Adequate Bench Space Provide bench or tabl...

Page 53: ...tor between the detector and the AC outlet Solvents Mobile Phase Use only HPLC grade solvents in all analyses HPLC grade water and methanol 1 liter each are required for performance verification Waste...

Page 54: ...arts from PerkinElmer s catalog service as described on the Customer Service page of this manual Table 2 1 Start Up Kit P N N292 0140 Part Number Description Quantity 0992 0513 ONE PIECE FINGERTIGHT T...

Page 55: ...Fuse The power input module located on the rear panel contains an outlet for the AC line cord the voltage selector card and the system fuse s Inside the power input module is a small voltage selector...

Page 56: ...tage selector card so that it is opposite the voltage selector arrow THIS NUMBER MUST CORRESPOND TO THE VOLTAGE FOR YOUR LOCATION Figure 2 1 shows the voltage selector card settings for both the 120 V...

Page 57: ...r Figure 2 2 Fuse Location Installing the AC Line Cord 3 Warning Ground circuit continuity is vital for safe operation of equipment Never operate equipment with grounding con ductor disconnected Do no...

Page 58: ...ord plug into the AC supply Figure 2 3 AC Line Cord Selections North America NEMA 5 15 0998 8986 Europe CEE 7 Schuko 0999 1415 Europe Switzerland 0999 1413 Europe Italy 0999 1422 Old British Standard...

Page 59: ...Function Location Power Connector Universal male AC line connector for power cord input contains the line fuse and voltage selector Rear Panel Fuses Time delay fuses 5 x 20 mm for protection of the S...

Page 60: ...ems have a 1 V FS input If the COMP output is set to 1 V AU the data system will have a calibration of 1 AUFS If small 1 AU peaks are to be observed this gives maximum sensitivity Large peaks however...

Page 61: ...ng this input from an external contact closure starts the method This performs the same action as the Run key You can run a method when READY appears on the top line of the method display EXT STOP Act...

Page 62: ...Plug the modular connector into the I O panel connector on the Series 200 LC Autosam pler Figure 2 4 shows a typical connection between the Detector and the Series 200 Pump 4 Refer to Figures 2 5 for...

Page 63: ...Making Input Output Connections Installation 2 13 Figure 2 5 Connecting a Wire to the Modular Connector...

Page 64: ...Installation Making Input Output Connections 2 14 Figure 2 6 System Connection Diagram Series 200 LC Autosampler Series 200 LC Pump Series 200 UV VIS Detector and a Data System...

Page 65: ...used since there will normally be minimal backpressure on the outlet side of the cell Waste tubing PN 0250 6495 is included in the Start up Kit The Start up Kit contains a supply of Fingertight PEEK...

Page 66: ...Installation Making Tubing Connections 2 16 Figure 2 7 Cutting the 010 inch Tubing Blade Edge or Triangular File Scribe Line Smooth Jaw Pliers...

Page 67: ...peat the steps above Connecting the Column Outlet Tubing to the Detector 1 Stop the pump 2 Slide the Fingertight connector over the end of the tubing 3 Insert the end of the tubing into the inlet conn...

Page 68: ...and Their Functions Connection Function Sample In Connector Bulkhead union 1 l6 inch OD 0 010 inch ID for connection of column outlet tubing to heat exchanger inlet Sample Out Connector Bulkhead unio...

Page 69: ...1 16 inch tubing for a zero dead volume union This type of union makes it easy for you to disconnect the Autosampler from the pump Scavanger Column PerkinElmer Scavanger Column Kit Part No 0258 0204...

Page 70: ...tion Making Tubing Connections 2 20 Figure 2 8 provides an overview of typical tubing connections between the Detector and other instruments making up an LC system Figure 2 9 Typical System Tubing Con...

Page 71: ...w to use the detector s Manual mode of operation It includes the following topics Understanding Manual mode Viewing the Detector Manual Settings Screen Setting control parameters Scanning the chromato...

Page 72: ...de the wavelength range response time or peak width value and timed events You cannot save the parameters that you set in Manual mode The set of parameters that appear the first time you start the det...

Page 73: ...o manual or method operation press the CNFG softkey The Detector displays the Configure menu shown below To switch to manual operation from the Configure menu press the MAN softkey The Detec tor displ...

Page 74: ...nse Time RSP You select either PW or RSP when you configure the detector For more information see Chapter 6 Configuring the Detector Once you select PW or RSP the detector displays the available value...

Page 75: ...f the Screen Description 6 CNFG Softkey Use to display the Configure menu options For more information on configuring the detector see Chapter 6 Configuring the Detector 7 SCAN Softkey Use to perform...

Page 76: ...tings Screen For more information see Viewing the Detector Manual Settings Screen topic earlier in this chapter The wavelength field is highlighted 1 Use the numeric keypad to enter the wavelength in...

Page 77: ...in Chapter 8 Principles Press the right arrow key or the key to set the RSP and move to the TE1 field enter enter 4 The relay event may be either O C or M These letters indicate the following O Open...

Page 78: ...he Scan command tells the detector to scan across all wavelengths in the sample cell To best correlate the scan wavelength you can have the recorder mark the output at a specified interval F8 DETECTOR...

Page 79: ...ments For high resolution use a slow speed Range For the recorder output enter the desired range of the scan Value range 0 0005 to 3 00 Absorbance Units Full Scale AUFS For increased sensitivity use a...

Page 80: ...can press F8 The Detector displays the SCAN IN PROGRESS Screen STRT 3 When the scan is finished the detector turns off the recorder if controlled from TE2 and returns you to the Scan screen 4 Press th...

Page 81: ...VIS LC Detector It includes the following information Understanding Methods Viewing the Method Screen Editing the Current Method Creating a New Method Storing a Method Recalling a Method from the Dire...

Page 82: ...ring a chromatogram Once you store a method you can recall it and run it You can run an active method The active method is the method that is currently running on the Series 200 UV VIS LC Detec tor or...

Page 83: ...Method Operation Screen To set the Detector to Method operation press the CNFG softkey The Detector displays the Configure menu shown below CONFIGURE MENU F6 To switch from manual to method operation...

Page 84: ...nged This remains displayed until you store the method This field is blank when you are creating a new method 4 Detector Status One of the following appears NTRDY Indicates Not Ready This status appea...

Page 85: ...ent Method topic later in this chapter 7 Elapsed Time Indicates the time in minutes that has elapsed since the start of a run 8 Softkey Labels Displays the options available on this screen Softkey Des...

Page 86: ...rameters and timed events The control parameters allow you set values for the method such as a wavelength value and peak width value The timed events allow you to control the relay contact closures wh...

Page 87: ...onse Time topic in Chap ter 8 Principles The parameter can be entered in either Peak Width PW or Response Time RSP You select either PW or RSP when you configure the detector For more information see...

Page 88: ...splays the Steps in the method The caret highlights Step 1 EDIT 1 You can use the cursor arrow keys or the key to move from field to field In the TIME field enter a time value such as 40 00 to indicat...

Page 89: ...parameters for Step 1 you can press the key to go back to the Method Screen or you can add another Step to the method return 6 To add another Step to the method press the key or the right arrow key T...

Page 90: ...To edit the timed events follow the procedure below From the Method Screen press F2 to display the Method Edit Screen EDIT 1 Press F5 to move to the Timed Events Screen The data field for the Time is...

Page 91: ...on Momentary closures should be preceded with an Open event enter 5 To add another event press either the key or the key A second event will be added and the Time field for the next event will increme...

Page 92: ...reate a new method press the softkey The detector displays a one step method with the default operating parameters NEW 2 METHOD EDIT SCREEN 3 Use the cursor arrow keys or the key to move from field to...

Page 93: ...Once you store the method the method status switches from CHNGD changed to STORD stored The STORD status indicates the method is stored in memory When you store a method the method gets added to a li...

Page 94: ...hat appears corresponds to the next available method number 1 20 Press the key to accept the method number shown or use the numeric keys to type in a new value and then press enter enter 1 2 3 SAVE ME...

Page 95: ...ry lists the stored methods and shows you the method number status name and date and time the method was last modified Once you recall a method it becomes the current active method To recall a method...

Page 96: ...c key to make the selected method the active method The detector displays the selected method on the Method Screen RCL yes 1 F6 MAIN SCREEN Press F6 from the Main Screen to go to the Directory Screen...

Page 97: ...Directory to recall a stored method or to edit a copy of the current method and save it as a new method When you edit a stored method the changes are stored with the method The detector does not physi...

Page 98: ...making changes to the control parameters For more information see the Editing the Current Method section earlier in this chapter EDIT 3 4 Message line DIRECTORY SCREEN caret METHOD EDIT SCREEN To sto...

Page 99: ...follow the procedure below run reset METHOD SCREEN To run the current method press the key on the keypad run The detector runs the method and shows time passing in the TIME field The detector runs the...

Page 100: ...y state At run the X axis displays the chromatogram for the total analysis time of the running method The up down arrows decrease increase the Y scale by a factor of two over the range 1 0 to 0 001 AU...

Page 101: ...r describes how to use Sequences on your Series 200 UV VIS LC Detector It includes the following information About Sequences Defining a Sequence Running a Method in Sequence Stopping a Sequence Using...

Page 102: ...atically run a series of methods while the detector is unattended The following are the possible states for a sequence Display Edit The sequence appears on the display and can be edited The sequence s...

Page 103: ...ps 3 Sequence Status LINKD indicates that the sequence has been set up and is ready to run In the figure above the sequence status is S03 001 The S indicates Sequence The 03 indicates the current set...

Page 104: ...es for Defining a Sequence If you have not already done so you need to create and store in the Detector s Directory all of the methods that you intend to include in the sequence Procedures for creatin...

Page 105: ...hods you want to include in the sequence Follow the procedure below to view the methods in the detector s Directory F6 Press F6 from the Main Method Screen to go to the Directory Screen DIR 1 Using th...

Page 106: ...s for the SET press the right arrow key to move to the INJ field and enter the number The number of injections indicates the number of times to run the method To create another SET press either the ke...

Page 107: ...rt To delete the entire sequence so that you can create a new sequence press F8 Pressing the key deletes all sets in the sequence To delete a specific step select the step and press the key on the key...

Page 108: ...injections A linked sequence requires that you press the Run key to run each injection in the sequence A linked method cannot be edited After you press the system asks if you want to turn the lamp off...

Page 109: ...n key the detector runs the first method in the sequence When the first method has been run for the first injection the detector becomes ready for the next injection or the next method in the sequence...

Page 110: ...Screen To break or stop a sequence press the softkey Stopping a method also automatically stops or breaks a sequence Softkey Description SET Allows you to select a SET number and review the parameters...

Page 111: ...ries 200 UV VIS LC Detector It includes the following information Setting Configuration Options Setting up the Instrument Diagnostic Tests for Service Use Validating the Wavelength Calibration Checkin...

Page 112: ...tions 6 2 Setting Configuration Options Setting the Detector configuration options involves the following tasks which appear on the Configure menu Setting up the Instrument Running Diagnostic Tests Ch...

Page 113: ...Screen Contrast and Screen Saver Setting the Analog Output Setting the System Clock Adjusting the Smoothing Mode Setting up the Leak Detector You can perform all of these functions from the Instrument...

Page 114: ...tor Setting Configuration Options 6 4 F7 MAIN SCREEN CONFIGURE SCREEN From the Main Screen press F7 to move to the Configure Screen CNFG Press F1 to move to the Instrument Setup Screen INST 1 2 F1 INS...

Page 115: ...o move to the desired field From the Instrument Setup Screen press F1 to move to the Lamp Setup Screen The the LIMITS field is highlighted 1 2 3 To accept the entry and move to the next field you can...

Page 116: ...22 being the most bright SCREEN CONTRAST SCREEN From the Instrument Setup Screen press F2 to move to the Screen Contrast Screen SCRN 1 2 3 To increase the screen contrast press F4 To decrease the scr...

Page 117: ...to offset the value of the detector output to a positive value to allow for any potential negative drift Computer Output Allows to you to set the number of volts per absorbance unit Recorder Adjust A...

Page 118: ...ne If the signal drifts below the range it cannot be seen on the recorder To get the signal back into the range you can adjust the offset to a positive value 1 2 Use the cursor arrow key to navigate t...

Page 119: ...your data handling system to identify the required voltage 1 2 Use the cursor arrow keys to navigate to the COMPUTER OUTPUT field Select one of the following softkeys to change the value in the field...

Page 120: ...To test the recorder select one of these settings CALB Puts out 10 mv ZERO Sets the recorder output to zero volts 1 2 Use the cursor arrow key to navigate to the RECORDER ADJUST field Select one of th...

Page 121: ...in DATE 12 becomes highlighted Use the numeric keys to enter the new day To move to the month character field 15 in DATE press F2 and use the numeric keys to enter the new month Set the year and the...

Page 122: ...Width at Half Height in Seconds Allows you to set the smoothing parameter in terms of how far the peak rises For information on selecting RSP or PW as a control parameter see Chapter 3 Using Man ual M...

Page 123: ...entry is made in the error log of the appropriate error message CONT The system continues to run No change occurs in the detector s state An error message appears on the display The error message is c...

Page 124: ...creen LEAK From the Instrument Setup Screen press F8 to move to page two of the Instrument Setup Screen MORE 1 2 3 Use the cursor arrow keys or the key to move from field to field Press the corre spon...

Page 125: ...lectronic and mechanical performance of the Detector This section describes only how to access these options For more information on detector diagnostics contact your PerkinElmer service representativ...

Page 126: ...results from the detector When you select CHK from the Configure menu the Detector displays the Wave length Calibration Check Screen This screen allows you to run a check on the wavelength calibration...

Page 127: ...r the Holmium Oxide Filter and then runs the calibration test STRT 3 When the test is complete the Detector displays the results of the test and instructs you to remove the Holmium Oxide Filter Press...

Page 128: ...ctronic failure If replacing the lamp does not clear the problem please contact your local PerkinElmer service representative FAN FAILURE The system has detected that the fan is not turning the lamp w...

Page 129: ...er service representative A Short or Open error on the External Leak Detector may be caused by incorrect installation of the leak detector Check the installation If all appears well then the error is...

Page 130: ...the lamp hours if necessary for example you can reset the lamp hours when you install a new lamp in the Detector LOG 2 To view the Detector s error log press F8 A screen similar to the following appea...

Page 131: ...odic maintenance procedures that must be performed to keep the detector in optimal working condition The chapter also provides systematic pro cedures for isolating and repairing problems with the inst...

Page 132: ...e acceptable The procedure outlined here takes the user through a step by step maintenance check It must be remembered that the Series 200 UV VIS LC Detector is just a single component in a complete H...

Page 133: ...to keep it in proper working condition Routine maintenance consists of replacing the normal wear items such as the deuterium lamp and columns whenever you notice a degradation in performance The exit...

Page 134: ...roximately 25 mL of tetrahydrofuran THF through the flowcell via the bulkhead connections THF should be pumped directly into the bulkhead unions from the pump and out to waste Disconnect all other sys...

Page 135: ...5 Figure 7 1 Flowcell location Top front view of Detector Figure 7 2 Flowcell compartment Front view of Detector Inlet fitting Heat exchanger Flowcell door Outlet fitting Flowcell screw Heat exchange...

Page 136: ...ws on the exit aperture gradually and uniformly until the aperture pressure on the flowcell windows is removed Remove the screws com pletely Also remove the apertures and the windows from both sides F...

Page 137: ...lling the Flowcell First reconnect the flowcell to its connecting tubing and the bulkhead bracket The flowcell should now be reinstalled as a complete assembly Before final positioning of the flowcell...

Page 138: ...periodically cleaned with a soft cotton swab and a solvent such as methanol Cleaning the Photodiode Windows 1 Open the Access door 2 Loosen the knurled nut and swing open the front panel 3 Swing the...

Page 139: ...flowcell plate 4 Remove the window housings by inserting a large blade screwdriver into the slots in the window housings and turning carefully counter clockwise 5 Clean the windows and allow them to...

Page 140: ...retaining rings into the housing assembly 2 Place the completed window housing assembly into the flowcell clamp and retighten into the flowcell plate 3 Replace the sample and reference cells and close...

Page 141: ...ne cord from the AC outlet 2 Allow the detector to cool for 15 30 minutes before proceeding 3 Loosen the knurled nut on the back panel 4 Open the access door Avertissement Ultraviolet radiation from t...

Page 142: ...mp retainer spring 11 Grip the entire lamp with one hand while holding the detector steady with the other Slide the entire lamp assembly off the lamp mount 12 Unplug the lamp Figure 7 5 Preparing to s...

Page 143: ...Unfasten the lamp retainer spring 6 Grasp the entire lamp with one hand while holding the detector steady with the other Slide the lamp assembly from the lamp mount Figure 7 6 Locating the lamp Instal...

Page 144: ...excessive noise or drift Excessive noise and drift after a lamp change may indicate that the lamp is improperly seated Remove the lamp reinstall and recheck If any problems persist call your PerkinElm...

Page 145: ...the mobile phase between the HPLC pump and column out let because of the relatively high pressures in that area of the chromatographic system However these gases may bubble out of solution after leavi...

Page 146: ...matter Sonication may also be used to accomplish degassing Place the vessel containing the mixed mobile phase into a sonicator containing about 1 2 inch of water or more depending upon the size of th...

Page 147: ...tion Cer tain strategies can be used to minimize their negative effects UV Cutoff The UV cutoff of an HPLC grade solvent is usually defined as the wavelength at which the solvent exhibits 1 0 AU absor...

Page 148: ...Detector is set for the correct voltage 2 Turn on the power You should see the Power Up screen and the lamp should light If nothing happens disconnect the instrument from the power line and check the...

Page 149: ...ental Whenever a material is warmed or cooled its dimensions change The monochromator of any absorbance detector will follow the thermal changes of its environment with slight changes in its physical...

Page 150: ...ularly important when an instru ment is removed from a cold room to a high humidity room at ambient temperature Compressed gas cylinders must be safely attached to the wall or bench by means of approv...

Page 151: ...ak Check waste line for flow then trace tubing for a leak Contaminated mobile phase Replace mobile phase Wavelength setting below the cutoff wavelength of the mobile phase Select the correct wavelengt...

Page 152: ...vel is not too low Apply back pressure at outlet or connect a backpressure device P N 0990 7126 Air bubbles in the flowcell Degas the mobile phase Connect a backpressure valve P N 0990 7126 Bypass the...

Page 153: ...e with new column Peaks are broad loss of resolution Column losing efficiency Invert and flush the column If regeneration is unsuccessful replace and equilibrate with column Change in mobile phase Pre...

Page 154: ...ty flowcell Clean and or rebuild the flowcell High flowcell back pressure Plugged flowcell Clean or rebuild the flowcell Up scale drift higher absorbance levels Chromatographic problems Replace the fl...

Page 155: ...ents are covered by PerkinElmer for labor travel and service The following basic information should be available when calling the PerkinElmer service group In many cases successful troubleshooting can...

Page 156: ...Maintenance Troubleshooting Methods 7 26...

Page 157: ...principles of operation of the Series 200 UV VIS LC Detector and also provides some guidelines on determining appropriate values for key parameters Topics include Theory of Operation Application Notes...

Page 158: ...that certain solutions absorb light in an amount directly proportional to the concentration of solute presented to the light Bouger and Lambert discovered that when light was passed through a thin lay...

Page 159: ...n in the cell The linear relationships pre dicted by Beer s Law remain strictly true only for monochromatic single wavelength light beam Also the linear relationship between absorbance and concentrati...

Page 160: ...The Series 200 UV VIS LC Detector s dual beam optical design consists of either a deute rium for 190 360 nm or tungsten for 360 700 nm light source a patented monochromator a flowcell and reference ce...

Page 161: ...while maintaining a narrow bandwidth the Series 200 UV VIS LC Detector uses a bandwidth of 5 nm In the PerkinElmer dual beam design the intensities of the light passing through the sam ple flowcell an...

Page 162: ...and grat ing for wavelength selection Flowcell Flowcell supplied for sample path operation 12 L 8 mm path std Reference Dry Cell Dry cell supplied for reference path operation Stepper Motor Stepper mo...

Page 163: ...oosing a wavelength off of the absorbance maximum peak can also be used to decrease the molar absorptivity sensitivity for an analysis with concentrated solutes This will help increase the linearity o...

Page 164: ...imited In order to obtain the best signal to noise ratio S N an elec tronic filter is added to eliminate noise frequencies while allowing all signal frequencies to pass unattenuated However some frequ...

Page 165: ...ate and pressure variations teaks temperature variations aging lamps and gas in the detector cell Response Time Versus Time Constant The time constant value describes the degree of filtering of freque...

Page 166: ...baseline will contain much less short term noise However larger rise times may have the following effects on peaks Peak shape will become asymmetric The peak maximum will be shifted The peak height wi...

Page 167: ...wing information Configuring Turbochrom with the Series 200 UV VIS Detector The Series 200 UV VIS Detector Configured into Turbochrom as a 785 Detector The Series 200 UV VIS Detector Configured into T...

Page 168: ...nfigured into Turbochrom as a Series 200 UV VIS Detector In this mode the method capabilities of the Series 200 UV VIS Detector are available from Turbochrom enabling the use of wavelength changes dur...

Page 169: ...pplied to the digital chromatographic signal This offset is automatically removed by Turbo chrom in its chromatographic displays The offset is also present in both recorder and com puter output analog...

Page 170: ...Enabling External Control Configuring Turbochrom with the Series 200 UV VIS Detector 9 4...

Page 171: ...Scanning 3 8 Viewing 4 20 Cleaning Monochromator Exit Windows 7 9 Photodiode windows 7 8 Column scavanger 2 19 COMP and REC outputs 2 10 Computer Output 6 7 Adjusting 6 9 Computer Integrator Connecti...

Page 172: ...al Power providing sufficient 2 2 Electrical Safety Guidelines S 7 Electrical Warnings S 6 Environmental Considerations S 8 Error Log Viewing 6 18 Exposure Ultraviolet Radiation S 20 F F1 through F8 o...

Page 173: ...aintenance Guidelines 7 3 Making electrical connections 2 5 Making Plumbing Connections 2 15 Manual Mode Setting Operating Parameters in 3 6 Manual mode Switching to 3 3 Using 3 2 Manual Operation 1 8...

Page 174: ...RSP 4 7 Running a Method 4 19 Running a sequence 5 9 S Safety Electrical S 6 High voltage S 20 Moving the detector S 20 Suitability test S 13 Sample In Connector 2 18 Sample Out Connector 2 18 Satura...

Page 175: ...Guide 7 21 Tubing Connections Display of 2 20 Tubing connections 2 15 Tubing Connections making 2 15 Tubing Connections making external 2 19 Tubing Preparing to cut 2 15 Tungsten Lamp 6 5 8 6 Turbochr...

Page 176: ...Index In 6...

Page 177: ...kinelmer com The most up to date information on part numbers product brochures systems modules spare parts and application notes are in the PerkinElmer website Telephone U S only Call toll free 1 800...

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