PerkinElmer 200 UV/VIS Series, User Manual

Page: 153 / 177

Troubleshooting Methods Maintenance
7 - 23
Symptom Probable Cause Possible Remedy
Successive results
with the same sample
are not reproducible
Deteriorated chemical sample Visually check for change. make
fresh sample, clean flowcell,
and re-analyze.
Faulty injector valve Replace rotor seal.
Change in mobile phase Prepare new mobile phase.
Clean flowcell. Pump new
mobile phase until stable base-
line is achieved. Re-inject
sample.
Column losing efficiency
(peak broadening or
retention time changing) Invert and flush the column.
Replace and equilibrate with
new column.
Peaks are broad
(loss of resolution)
Column losing efficiency Invert and flush the column.
If regeneration is unsuccessful,
replace and equilibrate with
column.
Change in mobile phase Prepare a new mobile phase.
Clean flowcell. Pump the new
mobile phase until stable base-
line is achieved. Re-inject
sample.
Abnormal noise Chromatographic or electric
problems Install the “Dummy” cell
Install a power line filter.
If abnormal noise stops, check
for chromatographic problems.
If abnormal noise continues,
check the lamp or other
electrical diagnostics.
Column aging Install a new column
Bubbles in the flowcell Flush out the bubbles
Plumbing leaks Check all tubing connections for
tightness
Check the flowcell and column
outlet connection