PerkinElmer 200 UV/VIS Series, User Manual

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Making Tubing Connections Installation
2 - 15
Making Tubing Connections
The Series 200 UV/VIS LC Detector plumbing is easy to initially set up and provides trouble-
free operation. A piece of pre-cut (30 cm) .007” tubing (PN N293-1089) has been provided in
the Start up Kit for connection from the column outlet (or post column devices, if reaction
detectors are used) to the detector. The following items should be kept in mind when plumb-
ing the detector.
The outlet from the column should be connected to the IN connector with either a finger-
tightened type of compression fitting or a stainless steel compression nut and ferrule. Good
chromatographic practice dictates that the tubing used to connect the column outlet to the
SAMPLE IN connector be as short as possible to minimize band broadening. A length of
tubing should be connected in a similar manner to the detector at the OUT connector to
direct eluent to a waste collection container. Either stainless steel or plastic tubing may be
used since there will normally be minimal backpressure on the outlet side of the cell. Waste
tubing (PN 0250-6495) is included in the Start up Kit.
The Start up Kit contains a supply of Fingertight PEEK fittings (PN 0092-0513) for connect-
ing to the Detector inlet and outlet.
All PerkinElmer LC systems utilize connections made with stainless steel nuts, ferrules, and
tubing are rated to 6000 psi.
Preparing the .010-inch Tubing
You can use either .007-inch or .010-inch tubing for your tubing connection. It is strongly
recommended that you do not manually cut .007-inch tubing. Tubing that is smaller than
.010-inches in diameter is too small to cut manually without causing blockage.
To cut .010-inch tubing:
1. Measure a piece of tubing to your required length.
2. Scribe the tubing with a triangular or knife-edge file.
3. Firmly hold the tubing on each side of the scribe mark with smooth jaw pliers, then bend
the tubing back and forth to break (see Figure 2-6).
4. File any burrs off the end of the tubing.
All tubing used in the Series 200 UV/VIS LC
Detector is 316SS, 1/16-inch OD x 0.010-inch ID.
.007-inch tubing can be purchased in pre-cut
lengths from a PerkinElmer or other HPLC supply
catalogs.