PerkinElmer 200 UV/VIS Series, User Manual

Page: 79 / 177

Scanning the Chromatogram Using Manual Mode
3 - 9
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Parameter Possible Values
λ
BEG
Enter the wavelength where the
scan should begin.
Depends on the Configuration
options that have been set. For
example:
Deuterium Lamp: 190 to 360 nm
Tungsten Lamp: 360 to 700 nm
λ
END
Enter the wavelength where the
scan should end.
Depends on the Configuration
options that have been set. For
example:
Deuterium Lamp: 190 to 360 nm
Tungsten Lamp: 360 to 700 nm
Rate
Select a value for the scan speed.
The rate indicates the nm/second
that the wavelength changes. Range: 0.2 to 1.0 in 0.1 increments.
For high resolution, use a slow
speed.
Range
For the recorder output, enter the
desired range of the scan. Value range: 0.0005 to 3.00
Absorbance Units Full Scale (AUFS)
For increased sensitivity, use a low
range value. To keep a large peak on
the chart recorder, increase the
range value.
RSP or PW (depending on the
configuration)
Select a value for the response time
of the chromatogram. Value range: .02 to 1.0. Set this
value depending on how fast you are
scanning.
Tip: Start at fastest (or smallest)
value and increase if the detector is
too noisy.
Mark
Enter the mark interval. This is the
number of nanometers at which the
recorder leaves marks so that the
wavelength values can be matched
with output signals. If you type 0, the recorder leaves a
mark only at the beginning and end
of the scan.