PerkinElmer 200 UV/VIS Series, User Manual

Page: 152 / 177

Maintenance Troubleshooting Methods
7 - 22
Symptom Probable Cause Possible Remedy
Detector response is
NOT steady at any set-
ting
Loose lamp wires Check lamp wires
Faulty lamp Replace the lamp and align the
new lamp
Excessive line voltage
fluctuation Check your line voltage output
Inappropriate solvent Flush the entire system with
new solvent
Air bubbles in the solvent
inlet line Degas the solvent
Check that solvent level is not
too low
Apply back pressure at outlet or
connect a backpressure device
(P/N 0990-7126)
Air bubbles in the flowcell Degas the mobile phase.
Connect a backpressure valve
(P/N 0990-7126)
Bypass the column and pump
mobile phase at 5 mL/min (not
through the column) for 30 sec-
onds to dislodge air
Unstable sample Make a fresh sample, clean the
flowcell, then re-analyze
Dirty column Flush/regenerate column
Flowcell leaking Check the flowcell and repair or
replace if necessary
Leak in the flowcell area Tighten the column outlet fit-
ting if necessary
Tighten the detector waste line
for crimping and replace if nec-
essary