PerkinElmer 200 UV/VIS Series, User Manual

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Routine Maintenance Maintenance
7 - 7
Reassembling the Flowcell
1. Place a window in the seat in the rear of the flowcell body. Place the entrance aperture
(the one with threaded screw holes and the smaller center hole) onto the flowcell body
and window so the screw holes lineup with those in the flowcell body.
2. While holding these together, turn the flowcell body over and place another window in
the seat in the front of the flowcell body. Now place the exit aperture (the one with the
unthreaded screw holes and the larger center hole) onto the flowcell body and window so
that the screw holes line up with those in the flowcell body.
3. Insert the three screws and start each one until they engage the threads in the entrance
apertures.
4. Tighten each in turn a little at a time to ensure uniform tightening around the flowcell
windows. Tighten until resistance cannot be overcome without forcing.
Installing the Flowcell
First reconnect the flowcell to its connecting tubing and the bulkhead bracket. The flowcell
should now be reinstalled as a complete assembly. Before final positioning of the flowcell
into the detector, mobile phase should be pumped through the flowcell to ensure that no
leakage occurs.
Simply reverse the steps outlined above to remount the flowcell.
Please do not touch the quartz windows with fin-
gers. Skin oils will absorb UV and cause noise.
Be careful not to over or under tighten the
aperture screws. The windows may crack
and/or leak as a result.
Always use two 1/4-inch wrenches when tightening
or loosening the tubing to the flowcell. Use one to
keep the flowcell adapter from moving and the
other to tighten or loosen the compression nut on
the tubing. It is extremely critical not to over-
tighten the fittings since flowcells may be easily
damaged. A good rule of thumb is to make the con-
nections snug--not loose or tight, but just snug.
Always start mobile phase flow through the
newly installed flowcell and check all connec-
tions for leaks before closing the detector
head.