PerkinElmer 200 UV/VIS Series, User Manual

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Maintenance Routine Maintenance
7 - 4
Routine Maintenance
Flowcell Maintenance
Flowcell Flushing
The detector flowcell should be flushed thoroughly before and after each chromatographic
run with a volume of mobile phase equal to 10 times the volume of the installed flowcell. If
buffers are being used, the entire system should he flushed with HPLC grade water. Other-
wise, the flowcell may retain residues which could cause excessive noise or drift. The con-
tents of the detector flowcell may leave a residue upon drying out during long or short term
storage. Confirmed cases of flowcell contamination may necessitate the removal and disas-
sembly of the flowcell in order to clean or replace the flowcell windows.
Mild cases of flowcell contamination require a thorough flushing of the problem flowcell.
Flushing is a simple procedure. Pump approximately 25 mL of tetrahydrofuran (THF)
through the flowcell via the bulkhead connections. THF should be pumped directly into the
bulkhead unions from the pump and out to waste. Disconnect all other system components
(except the THF pump) before flushing the flowcell.
Removing the Flowcell
1. Turn off the Series 200 UV/VIS LC Detector and unplug the AC line cord from the AC
outlet.
2. Loosen the nut on the upper-right corner of the back panel.
3. Open the access door.
4. Unscrew the knurled screw that is holding the front panel.
5. Swing open the front panel to the right.
6. Slide the drawer out to the first stop. Be careful that the drawer is supported and will not
tip.
7. Remove the inlet and outlet fittings from the bulkhead.
8. Remove the screws that are holding the heat exchanger.
9. Open the flow cell door.
10. Loosen the screw that is holding the flowcell.
11. Remove the flow cell assembly.
If buffers are being used, flush the flowcell
with water to remove all buffers prior to THF
wash. Otherwise, buffer may precipitate and
clog the flowcell.