Chapter 5
Performing the Background Calibration and Optical Calibration
Performing the Optical Calibration for the 7500/7500 Fast System
Applied Biosystems 7300/7500/7500 Fast Real-Time PCR System Installation and Maintenance Guide
61
Notes
5
8.
Load the plate in the instrument as described in
“Loading the Plate” on page 40
.
IMPORTANT!
Wear powder-free
gloves when you handle the ROI
calibration plate.
Creating a Plate Document for the Optical Calibration Run
1.
Click
(or
select
File New
).
2.
Configure the New Document dialog box:
a.
Select
Assay Calibration
.
b.
Select
Container 96-Well Clear
.
c.
Select
Template Blank Document
.
d.
In the Operator field, enter your name.
e.
In the Comments field, enter any additional
information that you want to save to the file
(such as the plate bar code).
f.
In the Plate Name field, enter:
Calibration_
<
date in DDMMYY format
>
For example, the name for a calibration
performed on May 31, 2005 would be:
Calibration_310505.
g.
Click
.
3.
In the SDS software, select
File Save As
.
4.
In the Save As dialog box:
a.
If the Save in field does not display SDS
Documents, navigate to
D: drive Applied
Biosystems SDS Documents
.
b.
Click
.
“Performing the Optical Calibration”
Notched
corner
Well A1
12
GR2475
GR2475
7500
keyed corner
A1
A
B
C
D
E
F
G
H
1
2
3
4
5
6
7
8
9
10
11
12
A
1
7300/7500 System
Standard plate
7500 Fast System
Fast plate
a
b
c
d
e
g
f
Select
Should be
SDS Documents
Summary of Contents for 7300
Page 173: ......
