Appendix C
Determining Optimum Dye Concentration
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Notes
C
6.
In the SDS software, select
Instrument Calibrate
.
7.
In the warning dialog box, click
to
move the block.
8.
In the ROI Inspector, create the ROI image for
each filter, beginning with Filter A:
a.
In the Exposure Time field, enter
1024
.
b.
Click .
c.
Select
Filter A
.
d.
Click .
e.
Check the image for saturation.
f.
Record the coordinate of the well that
displays the brightest possible signal
without saturation. This well contains the
best concentration of the custom pure dye
for Filter A.
Select
Click
a
c
b
No Saturation
Normal
Saturation
Over Saturated
Good
Bad
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