Chapter 6
Performing the Pure Dye Calibration
Evaluating Pure Dye Calibration Spectra
80
Applied Biosystems 7300/7500/7500 Fast Real-Time PCR System Installation and Maintenance Guide
Notes
Evaluating Pure Dye Calibration Spectra
Use the tables in this section to verify that the peak for the spectra of the pure dye occurs
at the correct filter for your system:
•
7300
system
– see
•
7500
system
– see
•
7500
Fast
system
– see
If the peak for the spectra of the dye occurs in the wrong filter, you may have run the
wrong dye plate during the calibration. Repeat the procedures for only that dye.
Table 1
Spectra for pure dyes supported by the 7300 system
Instrument Pure
Dye
Filter
Peak
(nm)
7300 System
FAM
A
~520 nm
JOE B
~550 nm
NED C
~550 nm
Summary of Contents for 7300
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