Chapter 4
Performing the Regions of Interest (ROI) Calibration
Creating a Plate Document for the ROI Calibration
Applied Biosystems 7300/7500/7500 Fast Real-Time PCR System Installation and Maintenance Guide
43
Notes
4
5.
In the ROI Inspector dialog box, right-click the
black area of the window, then select
Display
Properties
.
6.
In the Image Viewer Control Properties dialog box:
a.
Select
Show Saturation
.
b.
In the Sat Threshold field, enter
4000
.
c.
Click
.
7.
In the ROI Inspector dialog box, set the lamp
control:
a.
Click
.
b.
Select
Idle
from the Lamp Control
drop-down list (sets the lamp to lower
voltage for ROI calibration).
“Performing the ROI Calibration” on
.
Select
b
c
a
b
a
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