Chapter 6
Performing the Pure Dye Calibration
Preparing for the Calibration
Applied Biosystems 7300/7500/7500 Fast Real-Time PCR System Installation and Maintenance Guide
71
Notes
6
Preparing for the Calibration
IMPORTANT!
Before performing a pure dye
calibration, you must perform an ROI calibration (see
), a background calibration (see
an optical calibration (7500 systems only, see
).
IMPORTANT!
Wear powder-free
gloves when you handle the pure dye
plates.
1.
Retrieve the spectral calibration kit from the
freezer, then remove all of the pure dye plates.
2.
Return the spectral calibration kit to the freezer.
3.
Allow the pure dye plates to warm to room
temperature (approximately 5 minutes).
IMPORTANT!
Do not remove a pure dye plate
from its packaging until you are ready to run it.
The fluorescent dye in the wells of each pure dye
plate is photosensitive. Prolonged exposure to
light can diminish the fluorescence signal
strength of the plate.
“Creating a Plate Document for the
Pure Dye Calibration” on page 72
.
Spectral calibration kit
FAM pure dye plate
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