Chapter 4
Performing the Regions of Interest (ROI) Calibration
Performing the ROI Calibration
Applied Biosystems 7300/7500/7500 Fast Real-Time PCR System Installation and Maintenance Guide
45
Notes
4
5.
Click .
The software takes a snapshot, then displays a
message dialog box or an ROI image:
6.
Click
.
The software saves the newly generated ROI
calibration for Filter A. “OK” appears next to
Filter A in the Masks Loaded section of the ROI
Inspector window.
If the software displays:
Do this:
a. Click .
b. Decrease the value in the Exposure Time field (see
) by half.
c. Repeat
and
.
A very faint ROI image or you cannot generate a
successful calibration.
See
.
An acceptable Calibration Image
Note:
A green circle indicates that the region of interest
for the well position is bright enough.
Verify that the:
• Wells Found value is 96.
• ROI Image displays a green circle around each well
area.
or
Successful calibration – All
wells have green circles
Successful calibration –
Wells Found must be 96
Click
Displays OK when
save is complete
Summary of Contents for 7300
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