Chapter 7
Verifying the Instrument Performance
Performing the Verification Run
Applied Biosystems 7300/7500/7500 Fast Real-Time PCR System Installation and Maintenance Guide
93
Notes
7
Performing the Verification Run
1.
Load the plate in the instrument as described in
“Loading the Plate” on page 40
.
Note:
When closing
the instrument tray,
apply pressure to the
right side of the tray
and at an angle.
2.
In the plate document:
a.
Select the
Instrument
tab.
b.
Click
.
The instrument begins the run, which lasts 1.5 to
2 hours using a 7300/7500 Real-Time PCR
system or <40 minutes using a 7500 Fast
Real-Time PCR system.
Note:
Before starting the run, the instrument
may pause (up to 10 minutes) to allow the heated
cover to reach the correct temperature.
3.
When the run is complete, click
.
.
Notched
corner
Well A1
12
GR2475
GR2475
7500
keyed corner
A1
A
B
C
D
E
F
G
H
1
2
3
4
5
6
7
8
9
10
11
12
A
1
7300/7500 System
Standard plate
7500 Fast System
Fast plate
Press
here
a
b
Click
Summary of Contents for 7300
Page 173: ......
