Chapter 7
Verifying the Instrument Performance
Preparing the TaqMan
®
RNase P Verification Plate
90
Applied Biosystems 7300/7500/7500 Fast Real-Time PCR System Installation and Maintenance Guide
Notes
Preparing the TaqMan
®
RNase P Verification Plate
IMPORTANT!
You must perform an ROI calibration
), a background calibration (see
and a pure dye calibration (see
) before
running an RNase P plate.
IMPORTANT!
Wear powder-free
gloves when you handle the RNase P
Verification plate.
1.
Retrieve the TaqMan RNase P Verification Plate
Kit from the freezer, remove the RNase P plate,
then allow the plate to warm to room temperature
(approximately 5 minutes).
2.
Remove the RNase P plate from its packaging.
Note:
If the RNase P plate contains a
compression pad, remove it from the plate.
Applied Biosystems does not recommend the use
of compression pads with the 7300/7500/7500
Fast system.
3.
Standard plates only
: Vortex the plate for
5 seconds. Do not vortex Fast plates.
(Remaining steps apply to both standard and Fast
plates.)
4.
Centrifuge the plate for 2 minutes in a centrifuge
with a plate adapter (<1500x
g
).
IMPORTANT!
The plate must be well mixed and
centrifuged.
>00:05:00
Ambient
RNase P
Verification Plate
00:00:05
<1500
×
g
Summary of Contents for 7300
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