Applied Biosystems 7300, Installation And Maintenance Manual

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Chapter 5 Performing the Background Calibration and Optical Calibration
Preparing the Background Calibration Plate
52
Applied Biosystems 7300/7500/7500 Fast Real-Time PCR System Installation and Maintenance Guide
Notes
Preparing the Background Calibration Plate
IMPORTANT!
Wear powder-free
gloves when you handle the
background plate.
1.
Retrieve the spectral calibration kit from the
freezer, then remove the prepared background
plate.
Alternatively, create a background calibration
plate as described in
Appendix B, “Creating a
Background Plate.”
2.
Return the spectral calibration kit to the freezer.
3.
Allow the background plate to warm to room
temperature (at least 5 minutes).
4.
Remove the background plate from its
packaging.
IMPORTANT!
Do not discard the packaging for
the background plate. The background plate can
be used up to three times if it is stored in its
original packaging sleeve.
5.
Standard plates only : Vortex the plate for
5 seconds. Do not vortex Fast plates.
(Remaining steps apply to both standard and Fast
plates.)
6.
Briefly centrifuge the background plate in a
centrifuge with a plate adapter (<1500x
g ).
IMPORTANT!
The plate must be well mixed and
centrifuged.
Spectral calibration kit
Background plate
>00:05:00 Ambient
00:00:05 <1500
×
g