Chapter 7
Verifying the Instrument Performance
Analyzing the TaqMan
®
RNase P Plate Data
96
Applied Biosystems 7300/7500/7500 Fast Real-Time PCR System Installation and Maintenance Guide
Notes
c.
In the Well Inspector, select
Omit Well
.
d.
Click
(or select
Analysis Analyze
) to
reanalyze the run without the outlying data.
8.
Repeat step 7 on the previous page for other
wells with outlying data.
IMPORTANT!
Up to six wells from each
replicate group in a RNase P plate can be omitted
to meet specifications. If the analysis contains
more than six outliers, troubleshoot the RNase P
verification run. See
.
9.
In the Results tab, select the
Standard Curve
tab.
10.
In the Standard Curve tab, verify that the R2
value is
≥
0.990.
11.
In the Results tab, select the
Report
tab.
Select
Omitted well
is crossed-out
Select
Must be
≥
0.99
Select
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