Chapter 7
Verifying the Instrument Performance
Overview
88
Applied Biosystems 7300/7500/7500 Fast Real-Time PCR System Installation and Maintenance Guide
Notes
Overview
Note:
You can also use the Real-Time System Setup wizard
to perform this procedure.
To access the wizard, select
Program Files 7300/7500/7500 Fast
System
Real-Time System Setup Wizard
.
Time Required
•
7300/7500 systems
– ~ 2 hours
•
7500 Fast systems
– ~ 40 minutes
Materials
Required
When to Verify
Instrument
Performance
Applied Biosystems recommends running a TaqMan
®
RNase P Instrument Verification
plate:
• When installing the system (you must perform all calibrations in this order: ROI
background optical (7500/7500 Fast systems only) pure dye instrument
verification).
• After moving the instrument to another location.
• As needed to verify the function of the 7300/7500/7500 Fast instrument.
Purpose of
RNase P Runs
The TaqMan RNase P Instrument Verification Plate run verifies the performance of an
Applied Biosystems 7300/7500/7500 Fast Real-Time PCR System. The RNase P plate is
pre-loaded with the reagents necessary for the detection and quantification of genomic
copies of the human RNase P gene (a single-copy gene encoding the RNase moiety of
the RNase P enzyme). Each well contains:
• Reaction mix:
–
7300/7500 systems:
1
✕
TaqMan
®
Universal PCR Master Mix
–
7500 Fast systems:
1
✕
TaqMan
®
Fast Universal PCR Master Mix
• RNase P primers
• FAM
™
dye-labeled probe)
• Known concentration of human genomic DNA template
Powder-free
Gloves
Safety
Goggles
TaqMan
®
RNase P
Instrument Verification
Plate
TaqMan
®
RNase P Instrument
Verification Plate Kit
GR2170
Centrifuge with plate
adapter
Summary of Contents for 7300
Page 173: ......
