Chapter 5
Performing the Background Calibration and Optical Calibration
Creating a Plate Document for the Background Calibration
54
Applied Biosystems 7300/7500/7500 Fast Real-Time PCR System Installation and Maintenance Guide
Notes
Creating a Plate Document for the Background Calibration
1.
In the SDS software, click
(or
select
File New
).
2.
Configure the New Document dialog box:
a.
Select
Assay Background
.
b.
Select
Container 96-Well Clear
.
c.
Select
Template Blank Document
.
d.
In the Operator field, enter your name.
e.
In the Comments field, enter any additional
information that you want to save to the file
(such as the plate bar code).
f.
In the Plate Name field, enter:
Background_
<
date in DDMMYY format
>
For example, the name for a plate run on
May 31, 2005 would be:
Background_310505.
g.
Click
.
3.
In the SDS software, select
File Save As
.
4.
In the Save As dialog box:
a.
If the Save in field
does not display SDS
Documents, navigate to
D: drive Applied
Biosystems SDS Documents
.
b.
Click
.
.
a
b
c
d
e
g
f
Should be
SDS Documents
Summary of Contents for 7300
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