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Doc. 065378-01 1/12

i

1 • Introduction

. . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . 1

1.1

Detector Overview . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . 1

1.2

Theory of Photodiode Array Detection  . . . . . . . . . . . . . . . . . . . . . . . . . . 2

1.2.1

Advantages of Photodiode Array Detection . . . . . . . . . . . . . . . . 3

1.3

Operator’s Manual Overview   . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . 6

1.4

Safety and Regulatory Information  . . . . . . . . . . . . . . . . . . . . . . . . . . . . . 7

1.4.1

Safety Messages and Notes   . . . . . . . . . . . . . . . . . . . . . . . . . . . . 7

1.4.2

Safety Labels   . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . 9

1.4.3

Declaration of Conformity  . . . . . . . . . . . . . . . . . . . . . . . . . . . . . 9

2 • Description

. . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . 11

2.1

Front Features  . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . 11

2.2

Inside Front Panel  . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . 13

2.3

Optical System . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . 14

2.3.1

Flow Cells . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . 16

2.4

Electronics  . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . 20

2.5

Rear Panel  . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . 22

2.6

Operating Guidelines . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . 25

2.6.1

Mobile Phases . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . 25

2.6.2

Solvent Delivery System   . . . . . . . . . . . . . . . . . . . . . . . . . . . . . 26

Contents

Содержание 074114

Страница 1: ...Dionex ICS Series Photodiode Array Detector Operator s Manual Document No 065378 Revision 01 January 2012...

Страница 2: ...to use in the product operation This document is copyright protected and any reproduction of the whole or any part of this document is strictly prohibited except with the written authorization of The...

Страница 3: ...verview 6 1 4 Safety and Regulatory Information 7 1 4 1 Safety Messages and Notes 7 1 4 2 Safety Labels 9 1 4 3 Declaration of Conformity 9 2 Description 11 2 1 Front Features 11 2 2 Inside Front Pane...

Страница 4: ...Connect to the Chromeleon 7 Client 34 3 1 3 Connect to the Chromeleon 6 8 Client 35 3 1 4 Turn On the Lamps 37 3 2 System Equilibration 37 3 3 Routine Operation 38 3 3 1 Direct Control 38 3 3 2 Automa...

Страница 5: ...rge or Small 84 4 13 Poor Peak Shape 84 4 14 Faulty USB Communication 86 4 14 1 Moduleware Run Time Diagnostics 87 4 14 2 Software Diagnostics and Calibration 87 5 Service 91 5 1 Liquid Leaks 91 5 2 C...

Страница 6: ...rs 108 B Installation 111 B 1 Facilities Requirements 111 B 2 Unpacking 112 B 3 Installing the Flow Cell 113 B 4 Connecting the Waste Line 116 B 5 Connecting the Analog Outputs Optional 116 B 6 Connec...

Страница 7: ...the USB Device Drivers 124 B 10 Configuring the Dionex PDA in Chromeleon 125 B 10 1 Using the USB Auto Configuration Wizard 125 B 10 2 Selecting Configuration Properties 127 B 11 Starting the Chromel...

Страница 8: ...Dionex PDA Operator s Manual vi Doc 065378 01 1 12...

Страница 9: ...Dionex PDA can be used in numerous laboratory environments for routine analysis as well as for sophisticated research tasks A deuterium lamp optimizes the UV range 190 to 380 nm and a tungsten lamp o...

Страница 10: ...eon 6 8 Chromatography Data System Chromeleon 7 and Chromeleon 6 8 also provide data acquisition and data processing functions NOTE Thermo Scientific Dionex Chromeleon Xpress software can provide real...

Страница 11: ...be programmed and all compounds that absorb within this range can be detected in a single analysis Determination of the correct wavelengths in one analysis After all peaks have been detected the maxi...

Страница 12: ...ity obtained from two consecutive chromatographic analyses Based on 2D data or if the analyses had been performed with a conventional variable wavelength detector the peaks might have been misidentifi...

Страница 13: ...1 Introduction Doc 065378 01 1 12 5 Figure 1 3 Spectra of Pyrene and an Impurity Impurity Pyrene...

Страница 14: ...duction to the chromatography software Chromeleon required for detector control Chapter 3 Operation and Maintenance Instructions for routine Dionex PDA operation with Chromeleon and guidelines for opt...

Страница 15: ...Canada call the nearest Thermo Fisher Scientific office 1 4 1 Safety Messages and Notes This manual contains warnings and precautionary statements that can prevent personal injury and or damage to the...

Страница 16: ...ation de danger potentiel qui si elle n est pas vit e pourrait entra ner des blessures mineures mod r es galement utilis pour signaler une situation ou une pratique qui pourrait gravement endommager l...

Страница 17: ...PDA is in compliance with the following standards EN 61010 1 2001 safety CAN CSA C22 2 No 1010 1 92 A2 97 safety UL 61010C 1 2002 R8 02 safety and EN 61326 1997 A1 1998 EMC susceptibility and immunity...

Страница 18: ...Dionex PDA Operator s Manual 10 Doc 065378 01 1 12...

Страница 19: ...tions see Figure 2 2 Use the power button for routine on off control of the Dionex PDA NOTE The main power switch is on the Dionex PDA rear panel see Figure 2 8 Always leave the main power switch on u...

Страница 20: ...amp is firing The LED flashes every 0 5 second if a command is issued to turn on the lamp but the lamp fails to turn on see Section 4 3 If a subsequent attempt to turn on the lamp is successful the LE...

Страница 21: ...flow cell is located behind a cover leaks from the cell tubing connections will not be apparent immediately To prevent damage to detector components the bottom of the optical bench contains a built i...

Страница 22: ...the source lens After exiting the cell the light passes through the spectrograph lens to the filter paddle and is focused into the slit The light then passes through the slit to the grating where it i...

Страница 23: ...tainless steel semi micro cells in both PEEK and 316 stainless steel and semi preparative cells in PEEK only Spectrograph Lens Receives the light from the flow cell and focuses it onto the slit Filter...

Страница 24: ...PL Up to 1 0 mL min SEMI PREPARATIVE FLOW CELL Cell Material Cell Volume Cell Path Length Volume of Heat Exchanger Inlet Tubing Intended Flow Rate Range PEEK P N 064167 0 7 PL 0 4 mm N A 5 0 to 100 0...

Страница 25: ...with normal phase or chlorinated solvents these solvents will damage the cell Do not use a stainless steel flow cell with low pH solutions these solutions may cause corrosion contamination and metal l...

Страница 26: ...l is designed for applications with 2 to 3 mm ID separator columns and flow rates up to 1 0 mL min Figure 2 6 Semi Micro Flow Cell Assembly Semi Micro Cell Component PEEK Cell SST Cell Inlet tubing 0...

Страница 27: ...h semi preparative flow applications and flow rates of 5 0 to 100 0 mL min The 0 4 mm path length minimizes detector absorbance for concentrated peaks Figure 2 7 Semi Preparative Flow Cell Assembly Ce...

Страница 28: ...l utilisateur Toute r paration doit tre effectu e par un personnel qualifi utilisant des proc dures correctes de d charge lectrostatique Halten Sie sich von der Elektronik des Dionex PDA fern Die Ele...

Страница 29: ...mp board Preamp output is directed to the Data Processing board Data Processing Board The Dionex PDA Moduleware resides on the Data Processing board This board provides the digital signal processing a...

Страница 30: ...analog output settings will be either the factory defaults see Section 3 3 3 or the settings selected the last time the detector was controlled by the computer Drain Tube Connector Waste exits the de...

Страница 31: ...N 060977 includes one USB cable P N 063246 For installation instructions see Section B 7 4 Power Entry The Dionex PDA power entry is socketed for a modular power cord IEC 320 C13 The detector operates...

Страница 32: ...illez ce que la prise de base soit situ e install e pr s du module et facilement accessible STROMSCHLAGGEFAHR Zur Vermeidung von elektrischen Schl gen ist eine geerdete Steckdose zu verwenden Das Ger...

Страница 33: ...advantageous to locate the Dionex PDA ahead of the suppressor This location also limits suppressor exposure to backpressure When changing from a buffer to a different operating mobile phase be sure th...

Страница 34: ...olvents methanol moderate concentrations of acetonitrile etc are acceptable for a PEEK system Other solvents and high concentrations of acetonitrile require a stainless steel system All tubing connect...

Страница 35: ...matography Data System Both software products provide extensive instrument control data management reporting and compliance features In Chromeleon 7 an ePanel Set provides centralized system control Y...

Страница 36: ...378 01 1 12 functions The UV_Vis tab provides access to Dionex PDA functions as well as detailed status and diagnostics information Figure 2 9 shows the Dionex PDA ePanel in Chromeleon 7 Figure 2 9 Ex...

Страница 37: ...rol commands are executed as soon as they are entered See Section 3 3 for details about direct control With automated control you create a Chromeleon 7 instrument method or a Chromeleon 6 8 program An...

Страница 38: ...on license can be reviewed and processed on any Chromeleon client station 2 7 3 System Wellness and Predictive Performance System Wellness monitors the overall health of the chromatographic system It...

Страница 39: ...c 065378 01 1 12 31 Leak detection Wavelength verification System Wellness and Predictive Performance commands and parameters are available in the Chromeleon 7 Command window or the Chromeleon 6 8 Com...

Страница 40: ...Dionex PDA Operator s Manual 32 Doc 065378 01 1 12...

Страница 41: ...h a Dionex ICS 5000 or Dionex ICS 3000 system use the power button on the front of the Dionex Dual Pump DP or Dionex Single Pump SP for routine on off control To turn off the pump press and hold the p...

Страница 42: ...he taskbar click Start All Programs Chromeleon 7 Services Manager to open the Services Manager and click Start Instrument Controller 2 Start the Chromeleon 7 client a Click Start on the Windows taskba...

Страница 43: ...rver right click the icon and select Start Server When the server is running and data acquisition is not occurring the icon is gray If the Server Monitor icon is not on the taskbar click Start on the...

Страница 44: ...Control panel select the Dionex PDA tab on the panel tabset see Figure 3 1 d Verify that the Connected check box is selected If it is not click the box to connect the Dionex PDA to Chromeleon 6 8 NOTE...

Страница 45: ...o the temperature required for the application Set the detector wavelength s and turn on the lamp s Calibrate the wavelength s Monitor the pump pressure and verify that the reading is correct for the...

Страница 46: ...ions refer to the Chromeleon Help 3 3 1 Direct Control With direct control you select operating commands and parameters from the following locations Chromeleon 7 ePanel Sets or the Command window or C...

Страница 47: ...n of Chromeleon installed The display filter level Normal Advanced or Expert The options selected for the detector in the Properties dialog box in the Chromeleon 7 Instrument Configuration program or...

Страница 48: ...lect the desired filter on the context menu To open the Chromeleon 6 8 Commands dialog box 1 Open the Dionex PDA Control panel 2 Select Control Commands or press the F8 key The Commands dialog box app...

Страница 49: ...the main steps required to set up and run the detector automatically Refer to the Chromeleon Help for details about these steps NOTE Before creating a program review Section 3 4 for a description of...

Страница 50: ...settings are at the factory defaults listed in the table below These default settings are also in effect when the power is first turned on Rise time 2 0 seconds UV lamp On Visible lamp On Stand Alone...

Страница 51: ...ivity vs baseline noise Reference wavelength Baseline drift Reference bandwidth Baseline noise baseline drift Bunch width Spectral resolution peak match disk space Step Baseline noise rise time data c...

Страница 52: ...e signal and results in less short term noise see Figure 3 5 However a rise time that is too long may result in reduced peak heights and asymmetric peak shapes When set correctly the rise time signifi...

Страница 53: ...few seconds select a faster data collection rate for example 5 0 Hz If the data collection rate is too slow the start and end points of peaks are not accurately determined If the collection rate is to...

Страница 54: ...ace it provides complete information about the analytes and the spectra can be used to determine peak identity purity and wavelength maxima Bandwidth The bandwidth defines a wavelength range around th...

Страница 55: ...nex PDA The selected wavelength will be used as the reference wavelength The reference intensity will vary over time as the signal intensity changes For maximum compensation select a reference wavelen...

Страница 56: ...is ideal for gradient analyses where the baseline may shift This mode also helps compensate for fluctuations in ambient temperature caused by air conditioning To cancel the active reference mode ente...

Страница 57: ...dwidth 10 nm Reference bandwidth 50 nm active reference mode only Flowing water at 1 0 mL min 10 35 MPa 1500 psi Figure 3 7 Effect of Reference Wavelength on Baseline Drift Reference Wavelength Mode O...

Страница 58: ...ut may increase the chance that the sample absorbs in the reference bandwidth this will reduce sensitivity The reference wavelength plus one half the reference bandwidth must be within the sample wave...

Страница 59: ...he required data storage by approximately the reciprocal Do not select a bunch width when the spectrum of the compound has a fine structure the bunch width will reduce the spectral resolution Figure 3...

Страница 60: ...selected for each data collection rate Step Override In almost all cases the step setting selected by Chromeleon 6 8 is the preferred setting However it is possible to override this setting by manual...

Страница 61: ...en step intervals reports that value and noise is reduced This setting is recommended for most applications When Average is Off Chromeleon 6 8 reports the data points at the step interval The data poi...

Страница 62: ...execute the command to calibrate the intensity of the deuterium lamp Afterward check the lamp intensity again If the reading is still too low clean the flow cell see Section 5 2 or replace the lamp s...

Страница 63: ...e controlled devices While the system is in standby mode the detector lamps remain on Both processes maintain the equilibrated status of an instrument until the system is restarted 3 6 Routine Mainten...

Страница 64: ...Steel Systems and Components Document No 031152 Monitor baseline noise If the noise increases above an acceptable level especially after a lamp s has been in service for an extended length of time re...

Страница 65: ...wavelength verification test for both the deuterium and tungsten lamps approximately every 6 months see Section 4 14 2 If a lamp fails the test calibrate the wavelength see Section 4 14 2 and then run...

Страница 66: ...Dionex PDA Operator s Manual 58 Doc 065378 01 1 12...

Страница 67: ...anada call the nearest Thermo Fisher Scientific office 4 1 Alarms and Error Conditions The Moduleware the instrument control firmware installed in the Dionex PDA periodically checks the status of cert...

Страница 68: ...te calibration command during data acquisition Error page 65 Can t execute diagnostic command during data acquisition Error page 65 Can t execute x command during data acquisition Error page 65 Comman...

Страница 69: ...ror while attempting to turn off Abort page 70 UV lamp error while attempting to turn on Abort page 70 UV lamp failed to turn on for the required operation Abort page 70 Visible lamp control circuitry...

Страница 70: ...Wavelength 190 to 800 nm Reference wavelength 750 nm Reference bandwidth 50 nm These parameters are valid because 750 nm 25 nm 50 of 50 nm 775 nm which is less than 800 nm The following parameters are...

Страница 71: ...s than 500 nm The following parameters are NOT VALID Wavelength 190 to 600 nm Reference wavelength 750 nm Reference bandwidth 50 nm These parameters are invalid because 190 to 600 nm is a range of mor...

Страница 72: ...o troubleshoot This message appears if you attempt to select a parameter for the 3D field while data acquisition is in progress Wait until data acquisition is complete or stop the run and then change...

Страница 73: ...when the computer attempts to communicate with the detector Shut down the Chromeleon instrument controller or server and turn off the Dionex PDA power wait 30 seconds and then power up both the instr...

Страница 74: ...t Thermo Fisher Scientific for assistance A backup paddle alignment routine ensures that the Dionex PDA remains functional even if the sensor cannot detect the filter wheel position Thus if this is th...

Страница 75: ...mo Fisher Scientific personnel To troubleshoot This message appears if you enter a rise time setting that is invalid for a data collection rate of 20 Hz Select a valid rise time To troubleshoot This m...

Страница 76: ...id and dry the leak sensor before resuming operation If the cell leaks after you resume operation replace it see Section 5 6 To troubleshoot Follow the troubleshooting steps in Section 4 7 To troubles...

Страница 77: ...r an invalid setting for the bunch width Select a bunch width setting from 1 to 25 nm To troubleshoot The Dionex PDA can measure the absorbance spectrum from 190 to 800 nm Make sure both the minimum a...

Страница 78: ...ot pinched under the lamp cover 3 Check the number of hours the lamp has been in service Open the Chromeleon 7 Command window see page 31 or the Chromeleon 6 8 Commands dialog box see page 32 and then...

Страница 79: ...f you notice a shift in the absorbance signal during this time replace the lamp If the error message relates to the tungsten lamp execute the VisLampAge command If the tungsten lamp has been in operat...

Страница 80: ...st be performed by Thermo Fisher Scientific personnel To troubleshoot Reduce the data collection rate to less than 20 Hz or select a wavelength range that is d 310 nm UV lamp control circuitry error w...

Страница 81: ...Does Not Light Lamp LED Is Flashing Rapidly Lamp default setting is not in effect When the factory default settings are in effect the deuterium and tungsten lamps turn on automatically each time the d...

Страница 82: ...Setting or VisLampPowerOnSetting is On If the LED continues flashing replace the lamp See Section 5 7 or Section 5 8 for instructions on replacing the deuterium lamp or tungsten lamp respectively Dete...

Страница 83: ...e applications work or for detector validation allow at least 2 hours for the lamps to stabilize After installing a new lamp allow from 8 to 24 hours for the lamp to stabilize After changing mobile ph...

Страница 84: ...an 2000 hours If this occurs replace the lamp See Section 5 7 or Section 5 8 for instructions on replacing the deuterium lamp or tungsten lamp respectively Lamp incorrectly installed Make sure that bo...

Страница 85: ...erest See page 44 for more information Detector exposed to high vibration Optical detectors are sensitive to vibrations Make sure the Dionex PDA installation site is vibration free Filter paddle has m...

Страница 86: ...to temperature and light Do not operate the Dionex PDA unless the front cover lamp cover and flow cell cover are in place Flow cell heat exchanger not in place Check that the flow cell inlet tubing is...

Страница 87: ...LED DEUTERIUM UV or TUNGSTEN VIS is lighted If necessary turn on one of the lamps from Chromeleon or the TTL input Low light throughput 1 Remove the cell from the optical bench see Section 5 4 2 Check...

Страница 88: ...heck the Chromeleon Audit Trail If the Filter wheel sensor could not detect blocked position or Filter wheel sensor could not detect open position message is displayed see page 66 for instructions Fai...

Страница 89: ...UV_Calibration c Execute the Intensity command to calibrate the lamp intensity d Execute the Autozero command to run the autozero routine e If no error message is displayed flush the cell with the mob...

Страница 90: ...displayed flush the cell with deionized water or methanol until the autozero routine can be performed without error Lamp s needs replacement Check the number of hours the lamps have been in service 1...

Страница 91: ...program or the Dionex PDA Control panel 4 11 Low Spectral Resolution Bunch width too wide Select a bunch width setting of 1 nm and then check the resolution again Bandwidth too wide Select a bandwidt...

Страница 92: ...that the reagent flow rate is correct Reference bandwidth too wide Select a narrower reference bandwidth The reference bandwidth should not overlap the sample bandwidth 4 13 Poor Peak Shape Concentrat...

Страница 93: ...elect a bandwidth that provides the resolution required to achieve the desired sensitivity A wider bandwidth reduces baseline noise but may increase the chance that the sample absorbs in the reference...

Страница 94: ...solve the problem Click Cancel to exit the wizard Turn off the detector and unplug the USB cable from the computer Install Chromeleon Reconnect the USB cable to the computer and turn on the power to t...

Страница 95: ...s and Calibration Chromeleon includes comprehensive diagnostics for the Dionex PDA This section describes some of the most frequently used diagnostic procedures For more information refer to the Chrom...

Страница 96: ...cute the WavelengthCalibration command In Chromeleon 6 8 a Verify the following conditions no solvent is flowing through the cell the background absorbance is low and there are no bubbles in the light...

Страница 97: ...bleshooting Doc 065378 01 1 12 89 In Chromeleon 6 8 a To open the Wellness panel click the Wellness button on the Dionex PDA Control panel b Click the wavelength verification script button under Diagn...

Страница 98: ...Dionex PDA Operator s Manual 90 Doc 065378 01 1 12...

Страница 99: ...roblem 5 1 Liquid Leaks The Dionex PDA is plumbed with the parts listed below For tightening requirements refer to Installation of Dionex Liquid Line Fittings Document No 031432 The manual is on the T...

Страница 100: ...97 N A Cell outlet tubing 0 38 mm 0 015 in ID P N 057304 N A Cell inlet tubing 0 12 mm 0 005 in ID N A P N 063903 Cell outlet tubing 0 25 mm 0 010 in ID N A P N 051650 Reducing union fittings P N 0559...

Страница 101: ...l with a succession of at least 10 PL each of deionized water acetone and 3 M HNO3 4 Reconnect the cell liquid lines 5 Flush the cell with deionized water for 20 to 30 minutes at 1 0 mL min 5 3 Removi...

Страница 102: ...ides and pull straight off to remove 2 Disconnect the tubing connected to the flow cell inlet and outlet see Figure 5 1 3 Remove the flow cell cover from the inside front panel see Figure 2 3 4 Standa...

Страница 103: ...low cell from the optical bench see Section 5 4 and place the cell on a clean work surface 2 Disassembling the cell with bare hands may introduce contaminants into the system Before proceeding put on...

Страница 104: ...n turn an additional one quarter turn 9 Repeat Step 3 through Step 8 to install the second window 10 Connect the flow cell to the pump and pressurize to 1 38 MPa 200 psi Check for leaks around the win...

Страница 105: ...ce stains the cell interior and cleaning the cell see Section 5 2 does not remove the stain or if excessive backpressure cracks the fused silica windows and you do not want to replace them see Section...

Страница 106: ...amp emits UV radiation that is harmful to the eyes Always turn off the detector power before removing the lamp cover La lampe deuterium emettet de rayons ultraviolets qui sont dangeroux pour les yeux...

Страница 107: ...dust If necessary clean the lamp with IPA isopropyl alcohol 12 Line up the flange of the new lamp with the two locating pins and three threaded holes in the optical bench see Figure 5 3 When the flan...

Страница 108: ...troller or server 21 Reset the lamp lifetime a Open the Chromeleon 7 Command window see page 31 or the Chromeleon 6 8 Commands dialog box see page 32 b Select UV and then select the UVLampAge command...

Страница 109: ...sible lamp connector and disconnect it from the 4 pin bulkhead connector on the side wall see Figure 5 3 10 Use a screwdriver to loosen the tungsten lamp screws and then pull the mounting assembly awa...

Страница 110: ...reinstall the tubing bracket 16 Replace the flow cell cover and reconnect the inlet and outlet tubing 17 Replace the detector front cover 18 Turn on the detector power 19 Start the Chromeleon instrum...

Страница 111: ...center to release it The fuse holder will pop out slightly when the lock is released Repeat for the other lock Pull the fuse holder straight out of the compartment HIGH VOLTAGE Disconnect the main po...

Страница 112: ...r Scientific recommends always replacing both fuses 6 Reinsert the fuse holder into the compartment The fuse holder is keyed to fit only in the correct orientation Apply pressure evenly against the ho...

Страница 113: ...r 100 W Maximum line draw 3 5 A at 110 VAC at power up Fuses Two fast blow IEC 127 fuses P N 954763 rated at 4 0 A Dimensions Height 17 4 cm 6 8 in Width 44 4 cm 17 5 in Depth 50 3 cm 19 8 in Clearanc...

Страница 114: ...rative Cell Installed 10 PAU at 254 nm 2 second rise time 4 nm bandwidth and flowing water at 1 0 mL min 15 PAU at 520 nm 2 second rise time 10 nm bandwidth and flowing water at 1 0 mL min Noise with...

Страница 115: ...use bases stronger than 0 1 M these solutions will etch the fused silica windows of the flow cell Do not use normal phase or chlorinated solvents with the PEEK flow cell these solutions will damage th...

Страница 116: ...ns may cause corrosion contamination and metal leaching Cell Body PEEK Volume and Optical Path Length 0 7 PL volume 0 4 mm 0 02 in path length Maximum Operating Pressure 10 34 MPa 1500 psi Chemical Co...

Страница 117: ...A Specifications Doc 065378 01 1 12 109 Volume of Semi Micro Cell Heat Exchangers including cell inlet tubing PEEK cell 5 PL Stainless steel cell 5 PL...

Страница 118: ...Dionex PDA Operator s Manual 110 Doc 065378 01 1 12...

Страница 119: ...bration free workbench of a height that allows convenient access to the interior of the Dionex PDA Allow at least 6 cm 2 4 in behind the Dionex PDA for power connections and ventilation Do not block a...

Страница 120: ...place B 2 Unpacking Carefully remove the Dionex PDA from the shipping container Do not turn on the Dionex PDA unless it is at room temperature 4 to 40 C 40 to 104 F If you move the detector from a col...

Страница 121: ...inlet tubing through the tubing guide on the lamp cover and reinstall the tubing bracket see Figure 5 1 6 Thread the flow cell outlet tubing through the slot on the side of the flow cell cover Replace...

Страница 122: ...Standard or Semi Micro Flow Cell Connections Figure B 2 Semi Preparative Flow Cell Connections Cell Inlet Tubing Reducing Union Fitting PEEK cell P N 055903 Stainless steel cell P N 055902 Reducing U...

Страница 123: ...ow rate of 1 0 mL min 2 meters for a flow rate of 0 5 mL min and so on c Install a 10 32 ferrule fitting and bolt on each end of the tubing d Using a union fitting P N 042627 connect the backpressure...

Страница 124: ...ionex PDA rear panel see Figure B 3 The four Dionex PDA analog outputs provide a 1 volt output maximum The outputs are controlled by absorbance wavelengths 1 through 4 in Chromeleon The chromatography...

Страница 125: ...put stage If the relay load can source more than 200 mA at 5V or higher it may damage the Data Processing board SHOCK AND FIRE HAZARD Inputs and outputs are not rated for connection to an AC power mai...

Страница 126: ...ter The Chromeleon 7 or Chromeleon 6 8 license is installed NOTE Thermo Fisher Scientific strongly recommends installing Chromeleon before connecting the Dionex PDA to the computer When Chromeleon is...

Страница 127: ...nt Controller The icon changes to gold to indicate that the Instrument Controller Service is starting When the Instrument Controller Service is running idle the icon changes to gray If the Chromeleon...

Страница 128: ...directly to the PC Connect the detector to an external USB hub P N 060392 To connect the Dionex PDA directly to the PC 1 Plug the A connector of the USB cable P N 063246 into the USB port on the compu...

Страница 129: ...1 Plug the A connector of the USB cable P N 063246 into the USB port on the module with the internal hub see Figure B 6 2 Plug the B connector of the USB cable into the USB receptacle on the Dionex PD...

Страница 130: ...lug the B connector of the USB cable into a port on the external USB hub see Figure B 7 The USB standard limits the USB cable length to 5 meters 5 5 yds Each USB device can be separated from the PC by...

Страница 131: ...t outlet is located near the Dionex PDA and is easily accessible Operation at AC input levels outside of the specified operating voltage range may damage the Dionex PDA DANGER D LECTROCUTION Pour vite...

Страница 132: ...zard automatically appears Accept the default option for Chromeleon 7 Create a new instrument or Chromeleon 6 8 Create a new timebase and click OK This opens the Auto Configuration dialog box Leave th...

Страница 133: ...d and then manually create an instrument or timebase For instructions on how to manually create an instrument refer to the Chromeleon 7 Help or Chromeleon 7 Installation Guide For instructions on how...

Страница 134: ...ange Timebase Names dialog box appears now Enter a new and unique name and click the Use Custom Names button To cancel click the Use Default Names button NOTE Selecting an instrument or timebase name...

Страница 135: ...roperties dialog box right click the detector in the instrument or timebase and click Properties The Properties dialog box for the detector appears see Figure B 8 2 Review the default settings for the...

Страница 136: ...d displays the ePanel Set 3 To view the Dionex PDA ePanel see Figure B 9 click the UV_Vis tab on the ePanel Set The ePanel provides access to Dionex PDA functions as well as detailed status and diagno...

Страница 137: ...ex PDA Control panel see Figure B 10 select the Dionex PDA tab on the panel tabset The Control panel provides access to Dionex PDA functions as well as detailed status and diagnostics information Figu...

Страница 138: ...Dionex PDA Operator s Manual 130 Doc 065378 01 1 12...

Страница 139: ...43276 Ferrule 043275 Nut 060498 Dionex AD25 Dionex PDA Dionex PDA 100 Flow Cell Windows Replacement Kit Standard Flow Cell 316 Stainless Steel 056126 Stainless steel standard flow cell with heat excha...

Страница 140: ...exchangers 051650 Stainless steel semi micro flow cell outlet tubing assembly 055902 Reducing union fitting stainless steel 010262 Ferrule 010261 Nut 060498 Dionex AD25 Dionex PDA Dionex PDA 100 Flow...

Страница 141: ...ture fluctuations 48 Analog output connectors 2 21 22 116 Default settings 42 Output 116 Analog output range 22 Analytes unknown 46 Audit Trail 13 59 Automated control 29 Average 43 Definition 53 Guid...

Страница 142: ...n 7 2 3D Data Acquisition license 30 Audit Trail 13 59 87 Automated control 41 Command window 39 Diagnostic procedures 87 Direct control 38 Direct control mode 38 ePanel Set 27 Installation 119 Instru...

Страница 143: ...anel Set 27 Equilibration system 75 Using the Smart Startup feature 38 Error messages 13 F Fan 21 68 Speed 21 Ferrule fittings 68 75 Part number 115 Filter paddle 14 15 Malfunction 77 80 Positions 15...

Страница 144: ...nt of detector 12 Clearing the ALARM LED 73 Lenses 15 Liquid leaks 91 Liquid lines Maintenance 55 56 Lithium battery disposal 91 M Maintenance procedures 55 Mise en garde icon 8 Mobile phases 26 Conce...

Страница 145: ...utdown procedure 55 Spare parts 131 Unpacking instructions 112 Warranty 91 Weight 105 Photodiode Array Panel ePanel 28 128 129 Post column reagents 84 Degassing 75 Power cord 23 103 123 POWER LED LED...

Страница 146: ...cement procedure 97 See also Cells Volume 16 107 Windows replacement 95 96 Windows replacement kit 95 Semi preparative flow cells 15 Chemical compatibility 108 Flow rate range 16 Heat exchanger volume...

Страница 147: ...83 Troubleshooting Baseline drift 78 79 Baseline noise 75 77 Elution time is slow 75 Lamp does not light 73 PDA does not respond 74 Peak shape is poor 84 Peak size is too big or small 84 Spectra not...

Страница 148: ...8 W Warning icon 7 Warnung icon 8 Warranty voiding 91 Waste line 22 Installation 116 Wavelength calibration 2 82 88 Causes of failure 82 95 Wavelength verification 15 82 88 Wavelengths single 1 46 Ref...

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