Thermo Scientific 074114, Руководство оператора

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4 • Troubleshooting
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procedures. If the detector fails the wavelength calibration again, contact
Thermo Fisher Scientific for assistance.
•
Deuterium lamp intensity out of calibration
1. Remove the cell from the optical bench (see Section 5.4 ).
2. Check the deuterium lamp intensity:
a. Open the Chromeleon 7 Command window (see page 31 ) or the
Chromeleon 6.8 Commands dialog box (see page 32 ).
b. Select UV , and then select LampIntensity .
If the lamp intensity reading is above 10 million counts for a standard or
semi-preparative cell
or above 3 million counts for a semi-micro cell,
follow these steps:
a. Open the Chromeleon 7 Command window (see page 31 ) or the
Chromeleon 6.8 Commands dialog box (see page 32 ).
b. Select UV , and then select UV_Calibration .
c. Execute the Intensity command to calibrate the lamp intensity.
d. Execute the Autozero command to run the autozero routine.
e. If no error message is displayed, flush the cell with the mobile
phase for the application and/or clean the cell as instructed in
Section 5.2 .
f. Reinstall the cell in the optical bench.
g. Autozero the detector again to verify that the problem has been
resolved. If the error message appears again, contact Thermo
Fisher Scientific for assistance.
If the lamp intensity reading is below 10 million counts for a standard or
semi-preparative cell
or below 3 million counts for a semi-micro cell,
follow these steps:
a. Check that the flange of the deuterium lamp is aligned with the
two locating pins in the optical bench (see Figure 5-3 ). If the
flange is aligned, the lamp is defective and should be replaced
(see
Section 5.7 ).
b. If the error message appears again, contact Thermo Fisher
Scientific for assistance.