Dionex PDA Operator’s Manual
2
Doc. 065378-01 1/12
Key features of the Dionex PDA include:
•
The deuterium and tungsten lamps ensure low baseline noise, high signal
intensity, and applications flexibility in the wavelength range from 190 to
800 nm.
•
The 1024-element photodiode array optimizes spectral resolution.
•
The built-in holmium oxide filter verifies wavelength calibration accuracy.
•
Five flow cells are available for standard, semi-micro, and semi-preparative
applications.
•
LEDs on the front cover provide detector status at a glance.
•
Four analog outputs support data collection on alternate data collection
systems.
•
The detector is controlled with a PC (personal computer) running Microsoft
®
Windows Vista
®
or Windows
®
XP and either the Chromeleon
®
7
Chromatography Data System or the Chromeleon
®
6.8 Chromatography Data
System. Chromeleon 7 and Chromeleon 6.8 also provide data acquisition and
data processing functions.
NOTE Thermo Scientific Dionex Chromeleon
®
Xpress software
can provide real-time control and monitoring of the
detector, but does not include data management
capabilities.
1.2
Theory of Photodiode Array Detection
Monochromatic variable wavelength detectors monitor eluting components of the
sample at a single wavelength (ideally, the wavelength of maximum absorbance).
Photodiode array detectors scan a range of wavelengths every few milliseconds
and continually generate spectral information. Wavelength, time, and absorbance
can all be plotted.
Photodiode array detectors provide three-dimensional information that allows an
accurate assessment of peak identity, purity, and quantitation in a single analysis.
Software support for photodiode array detectors includes peak purity and spectral
library search functions to help determine peak homogeneity and identity.
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