Thermo Scientific 074114, Руководство оператора

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Dionex PDA Operator’s Manual
88
Doc. 065378-01 1/12
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Wavelength calibration
Purpose: Improves wavelength accuracy in the UV range by
reassigning new wavelengths to each pixel, based on the emission
spectrum from the deuterium lamp. The detector will perform the
wavelength calibration routine and upload the result (pass or fail) to
Chromeleon. The results of both the initial calibration and the last
calibration are stored in the calibration log. You can view the results
at any time.
In Chromeleon 7:
a. Verify the following conditions: no solvent is flowing through the
cell, the background absorbance is low, and there are no bubbles
in the light path.
b. Open the Command window (see page 39 ), select UV , and
execute the WavelengthCalibration command.
In Chromeleon 6.8:
a. Verify the following conditions: no solvent is flowing through the
cell, the background absorbance is low, and there are no bubbles
in the light path.
b. To open the Wellness panel, click the Wellness button on the
Dionex PDA Control panel.
c. Click the wavelength script button under Calibration .
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Wavelength verification
Purpose: Tests the accuracy of the selected wavelength. The
accuracy is verified for the following wavelengths: 360.9, 418.8, and
536.5. The results of the test (pass or fail) are logged in the Audit
Trail. The result of the last wavelength verification test is stored in the
calibration log and can be retrieved at any time.
NOTE Wavelength verification can take up to
2 minutes. During this time, data acquisition is
not possible.
In Chromeleon 7:
a. Open the Command window (see page 39 ), click UV , and execute
the WavelengthVerTest command.