Biotek MultiFlo FX, Руководство оператора

Страница: 43 / 330

Performance Specifications | 21
Performance Specifications
Peri-Pump Dispenser
Precision is measured for a whole 96-well or 384-well plate (12- or 24-well plate for RAD
technology) using room-temperature deionized or distilled water with 0.1% Tween 20 with
FD&C #1 blue dye. Precision is measured for 1536-well plates by dispensing to 384 wells, 12
columns with a 15% isopropyl alcohol solution. The absorbance of the solution is read at 630
nm and 450 nm reference. Specifications apply to volumes that are full unit increments for the
cassette to which they apply, except the 1 µL cassette also supports 0.5 µL increments when
dispensing this volume. For example: the precision specification for a 10 μL cassette is valid
at 10, 20, 30, ..., 3000 μL; the 1 µL cassette precision specification is valid at 0.5, 1, 2, 3, ...,
30,000 µL.
Accuracy is measured gravimetrically when dispensing room-temperature deionized water.
Specifications apply to volumes that are full unit increments for the cassette to which they
apply. For example: the accuracy specification for a 10 µL cassette is valid at 10, 20, 30, ...
30,000 µL.
Cassette Precision Accuracy
1 µL 10%CV @ 1 µL per well ± 10% @ 1 µL per well
5%CV @ 2 µL per well* ± 5% @ 2 µL per well*
10%CV @ 0.5 µL per well n/a
5 µL 5%CV @ 5 µL per well ± 4% @ 5 µL per well
2.5%CV @ 10 µL per well* ± 2% @ 10 µL per well*
10 µL 4%CV @ 10 µL per well ± 4% @ 10 µL per well
2%CV @ 20 µL per well* ± 2% @ 20 µL per well*
* These specifications are for these dispense volumes and higher.
Exception: the accuracy specs above apply to the RAD technology bulk-dispensing cassette, 8
tubes-to-1 (chute) with 5 µL tubing for volumes that are 8 times the full unit increments for
the cassette, e.g. valid at 40, 80, 160, ... 30,000 µL:
l
± 4% when volume is 40 µL/well
l
± 2% when volume is ≥80 µL/well.
BioTek Instruments, Inc.