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222 | Chapter 5: Qualification
Tests – Solutions
Cassette Types
1 µL 1 µL 1536 5 µL 10 µL
1 µL √ √
5 µL √
10 µL √ √
50 µL √
100 µL √
1536 √
8-to-1 √
l
1 µL Test : Confirms the performance of the 1
µ
L cassettes when dispensing a single
aliquot (1/4 turn of pump) into each well of the plate. It dispenses 1
µ
L into each well
using the 1 µL Solution , and requires an additional 150 µL of deionized or distilled
water to raise the fluid level for optimal reading.
n
A single aliquot for a cassette type is the smallest volume unit recommended
for it. 1 µL for the 1 µL cassette, 5 µL for the 5 µL cassette, and 10 µL for the
10 µL cassette (except that later model instruments can dispense 0.5 µL/well
using a 1 µL cassette).
l
5 µL Test : Confirms the performance of the 5
µ
L cassettes when dispensing a single
aliquot (1/4 turn of pump) into each well of the plate. It dispenses 5
µ
L into each well
using the 5 µL Solution , and requires an additional 150 µL of deionized or distilled
water to raise the fluid level for optimal reading.
l
10 µL Test : Confirms the performance of the 1
µ
L cassettes when dispensing 10 aliquots
(2 1/2 turns of pump) and the 10
µ
L cassettes when dispensing a single aliquot (1/4 turn
of the pump) into each well of the plate. It dispenses 10
µ
L into each well using the 10
µL Solution , and requires an additional 100 µL of deionized or distilled water to raise
the fluid level for optimal reading.
l
50 µL Test : Confirms the performance of the 5
µ
L cassettes when dispensing 10 aliquots
(2 1/2 turns of pump) into each well of the plate. It dispenses 50
µ
L into each well using
the 50 µL solution , and requires an additional 100 µL of deionized or distilled water to
raise the fluid level for optimal reading.
l
100 µL Test : Confirms the performance of the 10
µ
L cassettes when dispensing 10
aliquots (2 1/2 turns of pump) into each well of the plate. It dispenses 100
µ
L into each
well using the solution called 100 µL solution , and requires an additional 50 µL of
deionized or distilled water to raise the fluid level for optimal reading.
l
1536 Test : Confirms the alignment of the tips; that the cassette is firing straight into
the wells. Dispenses 6 µL into columns 2, 4, 19-30, 45, 47 of a 1536-well plate using the
"1536 solution." Also requires performing the 1 µL Test described above.
MultiFlo™ FX Multi-Mode Dispenser