Biotek MultiFlo FX, Руководство оператора

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Peri-pump Dispense Precision and Accuracy Tests | 221
Peri-pump Dispense Precision and Accuracy Tests
Dispense Precision and Accuracy Specifications
Cassette
Precision
Accuracy
1 µL 10%CV @ 1 µL per well ± 10% @ 1 µL per well
5%CV @ 2 µL per well* ± 5% @ 2 µL per well*
10%CV @ 0.5 µL per well n/a
5 µL 5%CV @ 5 µL per well ± 4% @ 5 µL per well
2.5%CV @ 10 µL per well* ± 2% @ 10 µL per well*
10 µL 4%CV @ 10 µL per well ± 4% @ 10 µL per well
2%CV @ 20 µL per well* ± 2% @ 20 µL per well*
* These specifications are for these dispense volumes and higher.
Exception: the accuracy specs above apply to the RAD technology bulk-dispensing cassette, 8
tubes-to-1 (chute) with 5 µL tubing for volumes that are 8 times the full unit increments for
the cassette, e.g. valid at 40, 80, 160, ... 30,000 µL:
l
± 4% when volume is 40 µL/well
l
± 2% when volume is ≥80 µL/well.
n
Note : For IQ/OQ/PQ purposes you can add 1.0% additional tolerance to the
Precision %CV to accommodate various test solutions, off-peak wavelengths,
reader errors, and pipette errors.
Peri-pump Precision and Accuracy Testing Methodology
Tare an empty plate on a balance. Use the Peri-pump to dispense a quantity of
fluid with a known dye concentration to the wells. Weigh the plate to obtain the
weight of the fluid dispensed. Pipette deionized water on top of the dye to bring
the wells up to a more optically measurable volume (if necessary). Read the wells
in a microplate reader and determine the percentage Coefficient of Variance (%CV)
among all wells, and gravimetrically determine the accuracy of the volume
dispensed in each well (% Accuracy Error).
BioTek recommends performing two tests, one at the volume that matches the
cassette type and another that best represents the cassette type and dispense
volume most common to your applications:
BioTek Instruments, Inc.