Biotek MultiFlo FX, Руководство оператора, Страница: 131 / 330

Wash Parameters Table | 109
Option Description/Values range
Default
values
Number of wash
cycles
Each wash cycle first aspirates and then dispenses
fluid to and from the plate. 3
Aspirate
Travel Rate The rate at which the washer manifold travels down
into the wells. The selection range is 1 to 5 for non-
cell-based assays, from slowest to fastest. With
these rates, the tubes slow their descent as they
approach the defined aspirate height (Z Position) to
aid complete evacuation of the well. 3
For delicate, cell-based assays, the range is 1CW
(cell wash) to 4CW and 6CW. These rates minimize
turbulence in the wells. The tubes descend at a
constant rate to the specified height. Rate 6CW
creates the least disturbance and performs fastest.
Delay Amount of time the tubes stay at the aspirate height
before lifting out of the wells. Define a delay
between 0 - 5000 ms. Increasing the delay may
improve evacuation of the wells. 0
Positioning X- and Y- horizontal axes, Z- height (vertical) axis
can be adjusted to improve performance. The
default Z-axis setting is plate dependent. See
Z-
Axis: Default Dispense and Aspirate Heights .
Z = 91 for
96-well
plates;
X & Y = 0
Secondary aspirate Also called Crosswise Aspiration. First, the wells are
aspirated using the position defined above. The
aspirate tubes rise and then descend to the
secondary position to aspirate again. Employ this
option to improve evacuation. No
Final Aspirate A final aspiration is the last action performed to
completely evacuate the wells. Same parameters as
regular aspirate step. Yes
Dispense
Flow Rate The rate at which the fluid is dispensed from the
tubes. For cell-based assays, use rate 1 or 2 for
gentle washing. For normal dispensing, the range is
3-11, 3 is slowest and 11 is fastest. 5
Volume µL/well dispensed: See Washer Flow Rate on page
111 .
Plate type
specific
BioTek Instruments, Inc.