Biotek MultiFlo FX, Руководство оператора

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102 | Chapter 3: Operation
immediately after each aspiration in a wash cycle and/or after the final aspiration
step.
n
Secondary aspiration is also called Crosswise aspiration because it is typically
performed in a different location within the well. Reposition the tubes by
defining different X-, Y-, and/or Z-axis positions when enabling the secondary
aspiration for the most effective evacuation of the wells.
Edit the Wash step or stand-alone Aspirate step to add a secondary aspiration:
LHC Touch screen
1. Double click the step in the
protocol to open it for editing.
2. Click
Advanced Options
(for
Aspirate) and enable
Perform
Secondary Aspirate .
3. Apply a second aspiration to the
Final Aspirate , also, for best
evacuation results.
4. Optionally, adjust the position of
the manifold as it addresses the
wells.
1. Edit the Wash step or stand-alone
Aspirate step:
Protocols>Edit
.
2. Select
Advanced Options
(for
Aspirate) and enable
Perform
Secondary Aspirate
.
3. Use the
Align
tool to define a
crosswise aspirate position.
4. Apply a second aspiration to the
Final Aspirate
, also, for best
evacuation results.
For example, you may want to adjust the X- or Y- axes to get to hard-to-reach areas
of certain types of wells. Or, you may want to lower the height (reduce the Z-axis),
to better evacuate fluid.
Create or Modify a Protocol
Home> Protocols
To create or edit a protocol:
1. Select
Protocols
at the
Home
/
Maintenance
screen.
2. Press
Create
or highlight the protocol and press
Edit
.
3.
Name
:
o
New protocols
: Touch the name field to open a keyboard. Enter
a unique name for the protocol.
o
Editing protocols
: Press the Info button and touch the name
field to change the protocol name. This will create a new copy of
the protocol you are editing. Later you can delete the original
MultiFlo™ FX Multi-Mode Dispenser