Biotek MultiFlo FX, Руководство оператора, Страница: 44 / 330

22 | Chapter 1: Introduction
Cassette Expected Lifetime
Cassette Types
Cassette Life
Total Volume
1 µL 1000 384-well plates @ 5 µL/well 2,000 mL
5 µL 1000 96-well plates @ 50 µL/well 5,000 mL
10 µL 1000 96-well plates @ 100 µL/well 10,000 mL
With strict adherence to best practices and maintenance recommendations, this is
the typical longevity of the dispense cassettes.
Syringe Dispensers
Precision is measured for a whole 96-well or 384-well plate using room-temperature
deionized or distilled water with 0.1% Tween 20 with FD&C #1 blue dye. Precision is
measured for 1536-well plates by dispensing to 384 wells, 12 columns with a 15% isopropyl
alcohol solution. The absorbance of the solution shall be read at 630 nm and 450 nm
reference.
Accuracy is measured gravimetrically when dispensing room-temperature deionized water.
Dispense Precision
8-Tube
≤
2% CV when dispensing 100 µL/well
≤
5% CV precision at 20 µL/well
≤
5% CV precision at 40 µL/well **
** Tested in-house to <4.0% CV.
16-Tube
≤
2% CV when dispensing 100 µL/well
≤
2.5% CV precision at 80 µL/well***
≤
5% CV precision at 20 µL/well
≤
10% CV precision at 5 µL/well*
*unspecified for non-autoclavable syringe pumps.
*** Tested in-house to <1.6% CV.
32-Tube < 12% CV when dispensing 6 µL per tube
Dispense Accuracy
8-Tube For all volumes 2 µL or 1%, whichever is greater, at flow rate 2.
16-Tube For all volumes 2 µL or 1%, whichever is greater, at flow rate 2.
32-Tube
±
5% when dispensing 6 µL/well at flow rate 3.
MultiFlo™ FX Multi-Mode Dispenser