Biotek MultiFlo FX, Руководство оператора

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188 | Chapter 4: Maintenance
1. Fill a supply bottle with buffer solution. Connect the bottle to the washer or
dispenser.
2. Run the applicable DAY_RINSE protocol.
3. Enable AutoPrime for 60-minute intervals.
n
Flush the buffer from the system using DI water when the device will be idle
for an extended period, i.e. repeat steps 1 and 2 using water.
At the end of the day:
1. Fill a supply bottle with buffer. Connect the bottle to the washer or dispenser.
2. Run the applicable DAY_RINSE protocol three times.
3. Fill a supply bottle with deionized water. And repeat steps 1 and 2.
4. Perform your regular Overnight/Multi-Day Maintenance routine.
Weekly or As Needed use NaOH and HCl to remove proteins:
1. Flush the system with 0.1-0.5 N* NaOH (sodium hydroxide), followed by
neutralization with an equivalent normality (0.1-0.5 N) of HCl (hydrochloride).
2. Rinse well with deionized water to remove the HCl.
3. Run the applicable DAY_RINSE protocol three times with deionized water if
you plan to use the device immediately.
n
* N = Normal solution, which contains 1 ‘gram equivalent weight’ (gEW) of
solute per liter of solution. The gram equivalent weight is equal to the
molecular weight expressed as grams divided by the ‘valency’ of the solute.
Alternatively use an Enzyme-Active Detergent:
1. Mix an enzyme-active detergent according to the manufacturer’s directions to
fill a four-liter supply bottle. Connect the bottle to the washer’s Buffer valve A
or one of the Syringes. Connect a bottle of deionized or distilled water to
Buffer valve B to rinse the tubing.
2. Run the
W-DECONTAMINATE
or
S-DECONTAMINATE
protocol, as appropriate.
3. Respond to the
Delay
message, “Connect a bottle of water…”, leave the
detergent bottle connected and when ready, press
Continue
.
4. For the Syringes: connect a bottle with deionized or distilled water to the
pump and REPEAT the protocol.
5. When the protocol is completed, connect a bottle containing four liters of
deionized water and run W-DAY_RINSE or S-DAY_RINSE three times to flush
MultiFlo™ FX Multi-Mode Dispenser