Biotek MultiFlo FX, Руководство оператора

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Handling Special Plates and Mini-tubes | 135
The Peri-pump’s dispense volume is per tube or channel , not per well. This is the
most important fact to consider when using these special plates. The volume
defined in a Quick Dispense or protocol is the amount each tube will dispense to
the well.
When you use multiple tubes to address a well, specify the desired volume with
this multiple in mind. For example, two tubes can address each well in a 24-well
plate, so the "defined" volume must be half the desired volume. As always, be sure
to also consider the
Peri-pump’s optimal performance settings when designing the
dispense protocol, i.e. full aliquots are more accurate than fractions of an aliquot.
Another tool to consider using with special plates is the X- or Y-axis Dispense
Position setting. You may be able to use it to aim the dispense tubes to a certain
region of the well.
6-, 12-, 24-, and 48-Well Plates
When addressing these plates with the Peri-pump, BioTek recommends
experimenting with different dispense-tube-to-well configurations. For some plates,
multiple tubes can dispense to a single well. Conversely, dispense tubes can (and
sometimes, must) be removed from the supply vessel or from the cassette to
prevent them from missing the wells.
Testing at BioTek found the following capabilities:
Plate Type Columns/Rows Tubes per Well Tubes removed
6 Well 3 x 2 3 or 4 (4 and 5) or 0
12 Well 4 x 3 2 3 and 6
24 Well 6 x 4 2 0
48 Well 8 x 6 1 3 and 6
l
6-well plates with 2 rows: three or four tubes can fit in the wells. Remove tubes 4 and
5 to use just three tubes per well. Fewer tubes may be preferred to preserve cells in
certain assays.
l
12-well plates with 3 rows: two tubes can fit in the wells, so 6 tubes will be used.
Remove tubes 3 and 6.
l
24-well plates with 4 rows and large enough wells to support two tubes are the easiest
of these special plates. All 8 tubes can be used, 2 per well, for standard microplates.
Exception: the special
24-vial rack, PN 7212058 , requires removing the even numbered
tubes from the cassette or fluid supply.
l
48-well plates with 6 rows: requires two tubes, 3 and 6, to be removed.
n
Remember, when defining a run: dispense volume is per tube , not per
well. Divide the desired volume by the number of tubes in a well.
BioTek Instruments, Inc.