Biotek MultiFlo FX, Руководство оператора

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212 | Chapter 4: Maintenance
Calibrate the Backlash for Syringe Dispenser
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Plate-Type-Specific Syringe Manifolds : this procedure requires an
experienced user and both standard 8- and 16-channel manifolds to determine
the backlash for these special manifolds. Contact BioTek TAC for assistance.
If you have replaced a check valve and subsequently noticed a decline in
performance, recalibrating the backlash is recommended to restore accuracy in
dispense volumes.
Equipment Required
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Microplates: 384-well plates for testing the 16-tube dispensers (which can be
replaced with 96-well plates if more applicable for your lab); 1536-well plates
for testing the 32-tube dispensers; and 96-well plates for testing the 8-tube
dispensers.
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Precision balance with minimum capacity of 100 g and readability of 0.001 g
resolution
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Supply bottle with deionized water
Setup
While calibrating and testing, try to maintain a steady liquid level in the supply
bottle, keeping it half full. Start with more fluid to allow for priming. Connect the
supply bottle to the Syringe under test. Make sure the supply bottle is at the same
level as the dispenser.
Create a Dispense Protocol
Create a protocol that dispenses the correct volume for the manifold under test:
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16-tube manifold: 20 µL per well to a 384-well plate at Flow Rate 2 .
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32-tube manifold: 6 µL per well to a 1536-well plate at Flow Rate 2 .
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8-tube manifold: 20 µL per well to a 96-well plate at Flow Rate 2 .
Prime and Dispense
1. Run a Prime protocol, for example,
S-DAY_RINSE
or
S-DAY_RINSE_A&B
when
both Syringe pumps need calibration, to remove air bubbles from the tubing.
2. Place the plate on the balance, and tare the balance.
3. Place the plate onto the carrier and run the dispense protocol created above.
MultiFlo™ FX Multi-Mode Dispenser