Biotek MultiFlo FX, Руководство оператора, Страница: 260 / 330

Perform the Syringe Dispense Precision & Accuracy Test | 238
manifolds:
Skip these protocol development steps for the 32-tube dispensers. And,
because you will save the protocols, you only need to create them one
time for the other manifolds.
1. Define a Dispense step for each test for each manifold, A and B:
Standard Syringe Manifolds
Manifold
Type Plate
Type
Volume
(µL/wel
l)
Flow
rate
Pre-dispense (2 cyc)
Test 1: 16-Tube 384 20 2 20
8-Tube 96 40 2 40
Test 2: 16-Tube 384 80 2 80
8-Tube 96 160 2 160
Plate-Type Specific Manifolds
Parameters 6-Well 12-Well 24-Well 48-Well
Volume (µL/well) 5560 2240 1120 560
Flow Rate 5 5 5 5
Z-axis/Dispense Height 465 460 452 460
Pre-dispense (2 cycles) 320 220 160 320
2. Save the protocol.
4. Place a clean, empty microplate on the balance and tare the balance.
5. Place the microplate on the carrier and run the protocol (created in step 3 or
predefined).
6. Place the plate on the balance and record the
Total Dispense Weight
. This value
will be used to calculate the % Accuracy Error.
7.
For Test 1 Only
: Use a calibrated hand pipette or the Peri-pump to dispense
deionized water on top of the dye solution in the wells.
l
384-well: Pipette 60
µ
L/well (resulting in 80
µ
L/well)
l
96-well: Pipette 120
µ
L/well (resulting in 160
µ
L/well)
BioTek Instruments, Inc.